Construct: ORF TRCN0000489660
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021911.1_s317c1
- DNA Barcode:
- GTCCCGTAACCTCCCTTGCCCTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRC1 (9055)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489660
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9055 | PRC1 | protein regulator of cytoki... | NM_003981.4 | 99.8% | 99.8% | 282G>A;560C>A |
| 2 | human | 9055 | PRC1 | protein regulator of cytoki... | NM_199413.3 | 97.6% | 97.5% | 282G>A;560C>A;1748_1749ins42 |
| 3 | human | 9055 | PRC1 | protein regulator of cytoki... | XM_011522187.2 | 96.1% | 94.4% | (many diffs) |
| 4 | human | 9055 | PRC1 | protein regulator of cytoki... | XM_011522188.3 | 95.1% | 91.9% | (many diffs) |
| 5 | human | 9055 | PRC1 | protein regulator of cytoki... | XM_017022712.2 | 93.9% | 93.8% | 282G>A;560C>A;1458_1459ins111 |
| 6 | human | 9055 | PRC1 | protein regulator of cytoki... | XM_017022713.2 | 91.6% | 91.6% | (many diffs) |
| 7 | human | 9055 | PRC1 | protein regulator of cytoki... | XM_017022715.2 | 91.5% | 91.4% | 0_1ins156;126G>A;404C>A |
| 8 | human | 9055 | PRC1 | protein regulator of cytoki... | XM_005254987.3 | 91.1% | 90.1% | (many diffs) |
| 9 | human | 9055 | PRC1 | protein regulator of cytoki... | XM_006720759.2 | 90.6% | 90.3% | (many diffs) |
| 10 | human | 9055 | PRC1 | protein regulator of cytoki... | XM_011522191.3 | 90.6% | 90.3% | (many diffs) |
| 11 | human | 9055 | PRC1 | protein regulator of cytoki... | XM_006720760.2 | 90.3% | 90.1% | (many diffs) |
| 12 | human | 9055 | PRC1 | protein regulator of cytoki... | XM_011522189.2 | 90.2% | 88.6% | (many diffs) |
| 13 | human | 9055 | PRC1 | protein regulator of cytoki... | XM_017022716.2 | 88.4% | 88.5% | 282G>A;499_500ins171;1577_1578ins42 |
| 14 | human | 9055 | PRC1 | protein regulator of cytoki... | XM_017022714.2 | 87.8% | 86.2% | (many diffs) |
| 15 | human | 9055 | PRC1 | protein regulator of cytoki... | XM_011522190.3 | 87.1% | 85.6% | (many diffs) |
| 16 | human | 9055 | PRC1 | protein regulator of cytoki... | NM_001267580.2 | 84.5% | 83.5% | (many diffs) |
| 17 | human | 9055 | PRC1 | protein regulator of cytoki... | XM_017022717.1 | 82.7% | 81.7% | (many diffs) |
| 18 | human | 9055 | PRC1 | protein regulator of cytoki... | XM_011522192.2 | 79.2% | 77.7% | (many diffs) |
| 19 | mouse | 233406 | Prc1 | protein regulator of cytoki... | XM_006540856.3 | 85.5% | 84% | (many diffs) |
| 20 | mouse | 233406 | Prc1 | protein regulator of cytoki... | NM_145150.3 | 83.4% | 81.7% | (many diffs) |
| 21 | mouse | 233406 | Prc1 | protein regulator of cytoki... | NM_001285997.1 | 83% | 81.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1929
- ORF length:
- 1860
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gaggagaagt gaggtgctgg cggaggagtc catagtatgt ctgcagaaag 121 ccctaaatca ccttcgggaa atatgggagc taattgggat tccagaggac cagcggttac 181 aaagaactga ggtggtaaag aagcatatca aggaactcct ggatatgatg attgctgaag 241 aggaaagcct gaaggaaaga ctcatcaaaa gcatatccgt ctgtcagaaa gagctgaaca 301 ctctgtgcag cgagttacat gttgagccat ttcaggaaga aggagagaca accatcttgc 361 aactagaaaa agatttgcgc acccaagtgg aattgatgcg aaaacagaaa aaggagagaa 421 aacaggaact gaagctactt caagagcaag atcaagaact gtgcgaaatt ctttgtatgc 481 cccactatga tattgacagt gcctcagtgc ccagcttaga agagctgaac cagttcaggc 541 aacatgtgac aactttgagg gaaacaaagg cttctaggcg tgaggagttt gtcagtataa 601 agagacagat catactgtgt atggaagaat tagaccacac cccagacaca agctttgaaa 661 gagatgtggt gtgtgaagac gaagatgcct tttgtttgtc tttggagaat attgcaacac 721 tacaaaagtt gctacggcag ctggaaatgc agaaatcaca aaatgaagca gtgtgtgagg 781 ggctgcgtac tcaaatccga gagctctggg acaggttgca aatacctgaa gaagaaagag 841 aagctgtggc caccattatg tctgggtcaa aggccaaggt ccggaaagcg ctgcaattag 901 aagtggatcg gttggaagaa ctgaaaatgc aaaacatgaa gaaagtgatt gaggcaattc 961 gagtggagct ggttcagtac tgggaccagt gcttttatag ccaggagcag agacaagctt 1021 ttgccccttt ctgtgctgag gactacacag aaagtctgct ccagctccac gatgctgaga 1081 ttgtgcggtt aaaaaactac tatgaagttc acaaggaact ctttgaaggt gtccagaagt 1141 gggaagaaac ctggaggctt ttcttagagt ttgagagaaa agcttcagat ccaaatcgat 1201 ttacaaaccg aggaggaaat cttctaaaag aagaaaaaca acgagccaag ctccagaaaa 1261 tgctgcccaa gctggaagaa gagttgaagg cacgaattga attgtgggaa caggaacatt 1321 caaaggcatt tatggtgaat gggcagaaat tcatggagta tgtggcagaa caatgggaga 1381 tgcatcgatt ggagaaagag agagccaagc aggaaagaca actgaagaac aaaaaacaga 1441 cagagacaga gatgctgtat ggcagcgctc ctcgaacacc tagcaagcgg cgaggactgg 1501 ctcccaatac accgggcaaa gcacgtaagc tgaacactac caccatgtcc aatgctaCGG 1561 CCAATAGTAG CATTCGGCCT ATCTTTGGAG GGACAGTCTA CCACTCCCCC GTGTCTCGAC 1621 TTCCTCCTTC TGGCAGCAAG CCAGTCGCTG CTTCCACCTG TTCAGGGAAG AAAACACCCC 1681 GTACTGGCAG GCATGGAGCC AACAAGGAGA ACCTGGAGCT CAACGGCAGC ATCCTGAGTG 1741 GTGGGTACCC TGGCTCGGCC CCCCTCCAGC GCAACTTCAG CATTAATTCT GTTGCCAGCA 1801 CCTATTCTGA GTTTGCGAAG GATCCGTCCC TCTCTGACAG TTCCACTGTT GGGCTTCAGC 1861 GAGAACTTTC AAAGGCTTCC AAATCTGATG CTACTTCTGG AATCCTCAAT TCAACCAACA 1921 TCCAGTCCTA GAACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1981 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2041 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTC CCGTAACCTC CCTTGCCCTT 2101 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t