Transcript: Human NM_199413.3

Homo sapiens protein regulator of cytokinesis 1 (PRC1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-29
Taxon:
Homo sapiens (human)
Gene:
PRC1 (9055)
Length:
3030
CDS:
119..1939

Additional Resources:

NCBI RefSeq record:
NM_199413.3
NBCI Gene record:
PRC1 (9055)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_199413.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000165472 GCGAGTTACATGTTGAGCCAT pLKO.1 360 CDS 100% 2.640 3.696 N PRC1 n/a
2 TRCN0000280716 GCGAGTTACATGTTGAGCCAT pLKO_005 360 CDS 100% 2.640 3.696 N PRC1 n/a
3 TRCN0000165078 GCGCTGCAATTAGAAGTGGAT pLKO.1 938 CDS 100% 2.640 3.696 N PRC1 n/a
4 TRCN0000158609 CAGGAACATTCAAAGGCATTT pLKO.1 1361 CDS 100% 10.800 7.560 N PRC1 n/a
5 TRCN0000280793 CAGGAACATTCAAAGGCATTT pLKO_005 1361 CDS 100% 10.800 7.560 N PRC1 n/a
6 TRCN0000159416 GCCTAACTTAAAGATGTGAAT pLKO.1 2066 3UTR 100% 4.950 3.465 N PRC1 n/a
7 TRCN0000166004 GCCTGACTTCTCTACCACATA pLKO.1 2196 3UTR 100% 4.950 3.465 N PRC1 n/a
8 TRCN0000280792 GCCTGACTTCTCTACCACATA pLKO_005 2196 3UTR 100% 4.950 3.465 N PRC1 n/a
9 TRCN0000158904 CAGATCATACTGTGTATGGAA pLKO.1 656 CDS 100% 3.000 2.100 N PRC1 n/a
10 TRCN0000161833 GCACGAATTGAATTGTGGGAA pLKO.1 1340 CDS 100% 2.640 1.848 N PRC1 n/a
11 TRCN0000162697 CCTCCTGAAGAGAATGTCTTT pLKO.1 2426 3UTR 100% 4.950 2.970 N PRC1 n/a
12 TRCN0000280717 CCTCCTGAAGAGAATGTCTTT pLKO_005 2426 3UTR 100% 4.950 2.970 N PRC1 n/a
13 TRCN0000160679 CCTGAAGGAAAGACTCATCAA pLKO.1 298 CDS 100% 4.950 2.970 N PRC1 n/a
14 TRCN0000280715 CCTGAAGGAAAGACTCATCAA pLKO_005 298 CDS 100% 4.950 2.970 N PRC1 n/a
15 TRCN0000159775 GCAGCTTTGTTAAATTGTGTT pLKO.1 2506 3UTR 100% 4.950 2.970 N PRC1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_199413.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000469291 AATGTGGCGCTTGATACCTCTACG pLX_317 25.4% 97.6% 97.5% V5 282G>A;560C>A;1748_1749ins42 n/a
2 ccsbBroadEn_07350 pDONR223 100% 97.5% 97.4% None (many diffs) n/a
3 ccsbBroad304_07350 pLX_304 0% 97.5% 97.4% V5 (many diffs) n/a
4 TRCN0000489660 GTCCCGTAACCTCCCTTGCCCTTA pLX_317 20.4% 97.6% 97.5% V5 (not translated due to prior stop codon) 282G>A;560C>A;1748_1749ins42 n/a
5 ccsbBroadEn_11330 pDONR223 100% 94.8% 94.8% None (many diffs) n/a
6 ccsbBroad304_11330 pLX_304 0% 94.8% 94.8% V5 (many diffs) n/a
7 TRCN0000471579 CCGTCGCCTAATCAAGACTCAACG pLX_317 17.5% 94.8% 94.8% V5 (many diffs) n/a
Download CSV