Transcript: Human XM_011522188.3

PREDICTED: Homo sapiens protein regulator of cytokinesis 1 (PRC1), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PRC1 (9055)
Length:
2504
CDS:
151..1974

Additional Resources:

NCBI RefSeq record:
XM_011522188.3
NBCI Gene record:
PRC1 (9055)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011522188.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000165472 GCGAGTTACATGTTGAGCCAT pLKO.1 392 CDS 100% 2.640 3.696 N PRC1 n/a
2 TRCN0000280716 GCGAGTTACATGTTGAGCCAT pLKO_005 392 CDS 100% 2.640 3.696 N PRC1 n/a
3 TRCN0000165078 GCGCTGCAATTAGAAGTGGAT pLKO.1 970 CDS 100% 2.640 3.696 N PRC1 n/a
4 TRCN0000158609 CAGGAACATTCAAAGGCATTT pLKO.1 1393 CDS 100% 10.800 7.560 N PRC1 n/a
5 TRCN0000280793 CAGGAACATTCAAAGGCATTT pLKO_005 1393 CDS 100% 10.800 7.560 N PRC1 n/a
6 TRCN0000158904 CAGATCATACTGTGTATGGAA pLKO.1 688 CDS 100% 3.000 2.100 N PRC1 n/a
7 TRCN0000161833 GCACGAATTGAATTGTGGGAA pLKO.1 1372 CDS 100% 2.640 1.848 N PRC1 n/a
8 TRCN0000162697 CCTCCTGAAGAGAATGTCTTT pLKO.1 1909 CDS 100% 4.950 2.970 N PRC1 n/a
9 TRCN0000280717 CCTCCTGAAGAGAATGTCTTT pLKO_005 1909 CDS 100% 4.950 2.970 N PRC1 n/a
10 TRCN0000160679 CCTGAAGGAAAGACTCATCAA pLKO.1 330 CDS 100% 4.950 2.970 N PRC1 n/a
11 TRCN0000280715 CCTGAAGGAAAGACTCATCAA pLKO_005 330 CDS 100% 4.950 2.970 N PRC1 n/a
12 TRCN0000159775 GCAGCTTTGTTAAATTGTGTT pLKO.1 1989 3UTR 100% 4.950 2.970 N PRC1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011522188.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07350 pDONR223 100% 95.1% 91.9% None (many diffs) n/a
2 ccsbBroad304_07350 pLX_304 0% 95.1% 91.9% V5 (many diffs) n/a
3 TRCN0000469291 AATGTGGCGCTTGATACCTCTACG pLX_317 25.4% 95.1% 91.9% V5 (many diffs) n/a
4 TRCN0000489660 GTCCCGTAACCTCCCTTGCCCTTA pLX_317 20.4% 95.1% 91.9% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroadEn_11330 pDONR223 100% 91.1% 89.4% None (many diffs) n/a
6 ccsbBroad304_11330 pLX_304 0% 91.1% 89.4% V5 (many diffs) n/a
7 TRCN0000471579 CCGTCGCCTAATCAAGACTCAACG pLX_317 17.5% 91.1% 89.4% V5 (many diffs) n/a
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