Construct: ORF TRCN0000489666
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020909.1_s317c1
- DNA Barcode:
- CTGTACCGCGCATAGGTGACCTTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GRM8 (2918)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489666
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001127323.1 | 99.9% | 99.7% | 235A>G;1085T>A |
| 2 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001371085.1 | 99.9% | 99.7% | 235A>G;1085T>A |
| 3 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_000845.3 | 98.5% | 98.2% | (many diffs) |
| 4 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001371083.1 | 98.5% | 98.2% | (many diffs) |
| 5 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001371084.1 | 98.5% | 98.2% | (many diffs) |
| 6 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001371086.1 | 96.4% | 95% | (many diffs) |
| 7 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_011516094.1 | 81.8% | 81.2% | (many diffs) |
| 8 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_011516095.2 | 78.5% | 78.4% | 1_1delAins583;503T>A |
| 9 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_011516101.2 | 77.3% | 77.3% | 0_1ins615;470T>A |
| 10 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_011516102.2 | 77.3% | 77.3% | 0_1ins615;470T>A |
| 11 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001371087.1 | 76% | 75.7% | (many diffs) |
| 12 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_017012075.1 | 76% | 75.7% | (many diffs) |
| 13 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_017012076.1 | 76% | 75.7% | (many diffs) |
| 14 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_017012077.1 | 76% | 75.7% | (many diffs) |
| 15 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_017012078.1 | 76% | 75.7% | (many diffs) |
| 16 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_017012079.1 | 76% | 75.7% | (many diffs) |
| 17 | human | 2918 | GRM8 | glutamate metabotropic rece... | NR_028041.1 | 74.2% | (many diffs) | |
| 18 | human | 2918 | GRM8 | glutamate metabotropic rece... | NR_163850.1 | 69.7% | (many diffs) | |
| 19 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_011516103.2 | 69.2% | 69.1% | 0_1ins837;248T>A |
| 20 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_017012080.1 | 69.2% | 69.1% | 0_1ins837;248T>A |
| 21 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_024446736.1 | 69.2% | 69.1% | 0_1ins837;248T>A |
| 22 | human | 2918 | GRM8 | glutamate metabotropic rece... | NR_163849.1 | 68.4% | (many diffs) | |
| 23 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_011516108.2 | 59.8% | 58.2% | (many diffs) |
| 24 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001371088.1 | 50% | 49.5% | (many diffs) |
| 25 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | NM_008174.2 | 91.9% | 98% | (many diffs) |
| 26 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | XM_006504987.3 | 91.9% | 98% | (many diffs) |
| 27 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | XM_006504989.1 | 91.9% | 98% | (many diffs) |
| 28 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | XM_006504990.3 | 91.9% | 98% | (many diffs) |
| 29 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | XM_006504991.3 | 91.9% | 98% | (many diffs) |
| 30 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | NM_001311072.1 | 90.5% | 96.6% | (many diffs) |
| 31 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | XM_017321397.1 | 90.5% | 96.6% | (many diffs) |
| 32 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | XM_017321398.1 | 63.7% | 67.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2796
- ORF length:
- 2724
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtatgc gagggaaagc gatcagcctc ttgcccttgt ttcttcctct 121 tgaccgccaa gttctactgg atcctcacaa tgatgcaaag aactcacagc caggagtatg 181 cccattccat acgggtggat ggggacatta ttttgggggg tctcttccct gtccacgcaa 241 agggagagag aggggtgcct tgtggggagc tgaagaagga aaaggggatt cacagactgg 301 aggccgtgct ttatgcaatt gaccagatta acaaggaccc tgatctcctt tccaacatca 361 ctctgggtgt ccgcatcctc gacacgtgct ctagggacac ctatgctttg gagcagtctc 421 taacattcgt gcaggcatta atagagaaag atgcttcgga tgtgaagtgt gctaatggag 481 atccacccat tttcaccaag cccgacaaga tttctggcgt cataggtgct gcagcaagct 541 ccgtgtccat catggttgct aacattttaa gactttttaa gatacctcaa atcagctatg 601 catccacagc cccagagcta agtgataaca ccaggtatga ctttttctct cgagtggttc 661 cgcctgactc ctaccaagcc caagccatgg tggacatcgt gacagcactg ggatggaatt 721 atgtttcgac actggcttct gaggggaact atggtgagag cggtgtggag gccttcaccc 781 agatctcgag ggagattggt ggtgtttgca ttgctcagtc acagaaaatc ccacgtgaac 841 caagacctgg agaatttgaa aaaattatca aacgcctgct agaaacacct aatgctcgag 901 cagtgattat gtttgccaat gaggatgaca tcaggaggat attggaagca gcaaaaaaac 961 taaaccaaag tgggcatttt ctctggattg gctcagatag ttggggatcc aaaatagcac 1021 ctgtctatca gcaagaggag attgcagaag gggctgtgac aattttgccc aaacgagcat 1081 caattgatgg atttgatcga tactttagaa gccgaactct tgccaataat cgaagaaatg 1141 tgtggtttgc agaatactgg gaggagaatt ttggctgcaa gttaggatca catgggaaaa 1201 ggaacagtca tataaagaaa tgcacagggc tggagcgaat tgctcgggat tcatcttatg 1261 aacaggaagg aaaggtccaa tttgtaattg atgctgtata ttccatggct tacgccctgc 1321 acaatatgca caaagatctc tgccctggat acattggcct ttgtccacga atgagtacca 1381 ttgatgggaa agagctactt ggttatattc gggctgtaaa ttttaatggc agtgctggca 1441 ctcctgtcac ttttaatgaa aacggagatg ctcctggacg ttatgatatc ttccagtatc 1501 aaataaccaa caaaagcaca gagtacaaag tcatcggcca ctggaccaat cagcttcatc 1561 taaaagtgga agacatgcag tgggctcata gagaacatac tcacccggcg tctgtctgca 1621 gcctgccgtg taagccaggg gagaggaaga aaacggtgaa aggggtccct tgctgctggc 1681 actgtgaacg ctgtgaaggt tacaactacc aggtggatga gctgtcctgt gaactttgcc 1741 ctctggatca gagacccaac atgaaccgca caggctgcca gcttatcccc atcatcaaat 1801 tggagtggca ttctccctgg gctgtggtgc ctgtgtttgt tgcaatattg ggaatcatcg 1861 ccaccacctt tgtgatcgtg acctttgtcc gctataatga cacacctatc gtgagggctt 1921 caggacgcga acttagttac gtgctcctaa cggggatttt tctctgttat tcaatcacgt 1981 ttttaatgat tgcagcacca gatacaatca tatgctcctt ccgacgggtc ttcctaggac 2041 ttggcatgtg tttcagctat gcagcccttc tgaccaaaac aaaccgtatc caccgaatat 2101 ttgagcaggg gaagaaatct gtcacagcgc ccaagttcat tagtccagca tctcagctgg 2161 tgatcacctt cagcctcatc tccgtccagc tccttggagt gtttgtctgg tttgttgtgg 2221 atccccccca catcatcatt gactatggag agcagcggac actagatcca gagaaggcca 2281 ggggagtgct caagtgtgac atttctgatc tctcactcat ttgttcactt ggatacagta 2341 tcctcttgat ggtcacttgt actgtttatg ccattaaaac gagaggtgtc ccagagactt 2401 tcaatgaagc caaacctatt ggatttacca tgtataccac ctgcatcatt tGGTTAGCTT 2461 TCATCCCCAT CTTTTTTGGT ACAGCCCAGT CAGCAGAAAA GATGTACATC CAGACAACAA 2521 CACTTACTGT CTCCATGAGT TTAAGTGCTT CAGTATCTCT GGGCATGCTC TATATGCCCA 2581 AGGTTTATAT TATAATTTTT CATCCAGAAC AGAATGTTCA AAAACGCAAG AGGAGCTTCA 2641 AGGCTGTGGT GACAGCTGCC ACCATGCAAA GCAAACTGAT CCAAAAAGGA AATGACAGAC 2701 CAAATGGCGA GGTGAAAAGT GAACTCTGTG AGAGTCTTGA AACCAACAGT AAGTCATCTG 2761 TAGAGTTTCC GATGGTCAAG AGCGGGAGCA CTTCCTAGGA CCCAGCTTTC TTGTACAAAG 2821 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2881 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2941 ACGACTGTAC CGCGCATAGG TGACCTTGAC GCGTTAAGTC gacaatcaac ctctggatta 3001 caaaatttgt gaaagatt