Construct: ORF TRCN0000489666

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020909.1_s317c1
DNA Barcode:
CTGTACCGCGCATAGGTGACCTTG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
GRM8 (2918)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489666
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2918 GRM8 glutamate metabotropic rece... NM_001127323.1 99.9% 99.7% 235A>G;1085T>A
2 human 2918 GRM8 glutamate metabotropic rece... NM_001371085.1 99.9% 99.7% 235A>G;1085T>A
3 human 2918 GRM8 glutamate metabotropic rece... NM_000845.3 98.5% 98.2% (many diffs)
4 human 2918 GRM8 glutamate metabotropic rece... NM_001371083.1 98.5% 98.2% (many diffs)
5 human 2918 GRM8 glutamate metabotropic rece... NM_001371084.1 98.5% 98.2% (many diffs)
6 human 2918 GRM8 glutamate metabotropic rece... NM_001371086.1 96.4% 95% (many diffs)
7 human 2918 GRM8 glutamate metabotropic rece... XM_011516094.1 81.8% 81.2% (many diffs)
8 human 2918 GRM8 glutamate metabotropic rece... XM_011516095.2 78.5% 78.4% 1_1delAins583;503T>A
9 human 2918 GRM8 glutamate metabotropic rece... XM_011516101.2 77.3% 77.3% 0_1ins615;470T>A
10 human 2918 GRM8 glutamate metabotropic rece... XM_011516102.2 77.3% 77.3% 0_1ins615;470T>A
11 human 2918 GRM8 glutamate metabotropic rece... NM_001371087.1 76% 75.7% (many diffs)
12 human 2918 GRM8 glutamate metabotropic rece... XM_017012075.1 76% 75.7% (many diffs)
13 human 2918 GRM8 glutamate metabotropic rece... XM_017012076.1 76% 75.7% (many diffs)
14 human 2918 GRM8 glutamate metabotropic rece... XM_017012077.1 76% 75.7% (many diffs)
15 human 2918 GRM8 glutamate metabotropic rece... XM_017012078.1 76% 75.7% (many diffs)
16 human 2918 GRM8 glutamate metabotropic rece... XM_017012079.1 76% 75.7% (many diffs)
17 human 2918 GRM8 glutamate metabotropic rece... NR_028041.1 74.2% (many diffs)
18 human 2918 GRM8 glutamate metabotropic rece... NR_163850.1 69.7% (many diffs)
19 human 2918 GRM8 glutamate metabotropic rece... XM_011516103.2 69.2% 69.1% 0_1ins837;248T>A
20 human 2918 GRM8 glutamate metabotropic rece... XM_017012080.1 69.2% 69.1% 0_1ins837;248T>A
21 human 2918 GRM8 glutamate metabotropic rece... XM_024446736.1 69.2% 69.1% 0_1ins837;248T>A
22 human 2918 GRM8 glutamate metabotropic rece... NR_163849.1 68.4% (many diffs)
23 human 2918 GRM8 glutamate metabotropic rece... XM_011516108.2 59.8% 58.2% (many diffs)
24 human 2918 GRM8 glutamate metabotropic rece... NM_001371088.1 50% 49.5% (many diffs)
25 mouse 14823 Grm8 glutamate receptor, metabot... NM_008174.2 91.9% 98% (many diffs)
26 mouse 14823 Grm8 glutamate receptor, metabot... XM_006504987.3 91.9% 98% (many diffs)
27 mouse 14823 Grm8 glutamate receptor, metabot... XM_006504989.1 91.9% 98% (many diffs)
28 mouse 14823 Grm8 glutamate receptor, metabot... XM_006504990.3 91.9% 98% (many diffs)
29 mouse 14823 Grm8 glutamate receptor, metabot... XM_006504991.3 91.9% 98% (many diffs)
30 mouse 14823 Grm8 glutamate receptor, metabot... NM_001311072.1 90.5% 96.6% (many diffs)
31 mouse 14823 Grm8 glutamate receptor, metabot... XM_017321397.1 90.5% 96.6% (many diffs)
32 mouse 14823 Grm8 glutamate receptor, metabot... XM_017321398.1 63.7% 67.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2796
ORF length:
2724
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggtatgc gagggaaagc gatcagcctc ttgcccttgt ttcttcctct
121tgaccgccaa gttctactgg atcctcacaa tgatgcaaag aactcacagc caggagtatg
181cccattccat acgggtggat ggggacatta ttttgggggg tctcttccct gtccacgcaa
241agggagagag aggggtgcct tgtggggagc tgaagaagga aaaggggatt cacagactgg
301aggccgtgct ttatgcaatt gaccagatta acaaggaccc tgatctcctt tccaacatca
361ctctgggtgt ccgcatcctc gacacgtgct ctagggacac ctatgctttg gagcagtctc
421taacattcgt gcaggcatta atagagaaag atgcttcgga tgtgaagtgt gctaatggag
481atccacccat tttcaccaag cccgacaaga tttctggcgt cataggtgct gcagcaagct
541ccgtgtccat catggttgct aacattttaa gactttttaa gatacctcaa atcagctatg
601catccacagc cccagagcta agtgataaca ccaggtatga ctttttctct cgagtggttc
661cgcctgactc ctaccaagcc caagccatgg tggacatcgt gacagcactg ggatggaatt
721atgtttcgac actggcttct gaggggaact atggtgagag cggtgtggag gccttcaccc
781agatctcgag ggagattggt ggtgtttgca ttgctcagtc acagaaaatc ccacgtgaac
841caagacctgg agaatttgaa aaaattatca aacgcctgct agaaacacct aatgctcgag
901cagtgattat gtttgccaat gaggatgaca tcaggaggat attggaagca gcaaaaaaac
961taaaccaaag tgggcatttt ctctggattg gctcagatag ttggggatcc aaaatagcac
1021ctgtctatca gcaagaggag attgcagaag gggctgtgac aattttgccc aaacgagcat
1081caattgatgg atttgatcga tactttagaa gccgaactct tgccaataat cgaagaaatg
1141tgtggtttgc agaatactgg gaggagaatt ttggctgcaa gttaggatca catgggaaaa
1201ggaacagtca tataaagaaa tgcacagggc tggagcgaat tgctcgggat tcatcttatg
1261aacaggaagg aaaggtccaa tttgtaattg atgctgtata ttccatggct tacgccctgc
1321acaatatgca caaagatctc tgccctggat acattggcct ttgtccacga atgagtacca
1381ttgatgggaa agagctactt ggttatattc gggctgtaaa ttttaatggc agtgctggca
1441ctcctgtcac ttttaatgaa aacggagatg ctcctggacg ttatgatatc ttccagtatc
1501aaataaccaa caaaagcaca gagtacaaag tcatcggcca ctggaccaat cagcttcatc
1561taaaagtgga agacatgcag tgggctcata gagaacatac tcacccggcg tctgtctgca
1621gcctgccgtg taagccaggg gagaggaaga aaacggtgaa aggggtccct tgctgctggc
1681actgtgaacg ctgtgaaggt tacaactacc aggtggatga gctgtcctgt gaactttgcc
1741ctctggatca gagacccaac atgaaccgca caggctgcca gcttatcccc atcatcaaat
1801tggagtggca ttctccctgg gctgtggtgc ctgtgtttgt tgcaatattg ggaatcatcg
1861ccaccacctt tgtgatcgtg acctttgtcc gctataatga cacacctatc gtgagggctt
1921caggacgcga acttagttac gtgctcctaa cggggatttt tctctgttat tcaatcacgt
1981ttttaatgat tgcagcacca gatacaatca tatgctcctt ccgacgggtc ttcctaggac
2041ttggcatgtg tttcagctat gcagcccttc tgaccaaaac aaaccgtatc caccgaatat
2101ttgagcaggg gaagaaatct gtcacagcgc ccaagttcat tagtccagca tctcagctgg
2161tgatcacctt cagcctcatc tccgtccagc tccttggagt gtttgtctgg tttgttgtgg
2221atccccccca catcatcatt gactatggag agcagcggac actagatcca gagaaggcca
2281ggggagtgct caagtgtgac atttctgatc tctcactcat ttgttcactt ggatacagta
2341tcctcttgat ggtcacttgt actgtttatg ccattaaaac gagaggtgtc ccagagactt
2401tcaatgaagc caaacctatt ggatttacca tgtataccac ctgcatcatt tGGTTAGCTT
2461TCATCCCCAT CTTTTTTGGT ACAGCCCAGT CAGCAGAAAA GATGTACATC CAGACAACAA
2521CACTTACTGT CTCCATGAGT TTAAGTGCTT CAGTATCTCT GGGCATGCTC TATATGCCCA
2581AGGTTTATAT TATAATTTTT CATCCAGAAC AGAATGTTCA AAAACGCAAG AGGAGCTTCA
2641AGGCTGTGGT GACAGCTGCC ACCATGCAAA GCAAACTGAT CCAAAAAGGA AATGACAGAC
2701CAAATGGCGA GGTGAAAAGT GAACTCTGTG AGAGTCTTGA AACCAACAGT AAGTCATCTG
2761TAGAGTTTCC GATGGTCAAG AGCGGGAGCA CTTCCTAGGA CCCAGCTTTC TTGTACAAAG
2821TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
2881GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
2941ACGACTGTAC CGCGCATAGG TGACCTTGAC GCGTTAAGTC gacaatcaac ctctggatta
3001caaaatttgt gaaagatt

Download FASTA (ORF) (Full)