Construct: ORF TRCN0000489670
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020169.1_s317c1
- DNA Barcode:
- ATAAGCTCGGCGCCGATCATACCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ETNK1 (55500)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489670
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55500 | ETNK1 | ethanolamine kinase 1 | NM_001039481.2 | 100% | 100% | |
| 2 | human | 55500 | ETNK1 | ethanolamine kinase 1 | XM_005253420.4 | 55.4% | 52.7% | (many diffs) |
| 3 | human | 55500 | ETNK1 | ethanolamine kinase 1 | XM_017019581.1 | 47% | 43.9% | (many diffs) |
| 4 | human | 55500 | ETNK1 | ethanolamine kinase 1 | NM_018638.5 | 43.7% | 40.7% | (many diffs) |
| 5 | human | 55500 | ETNK1 | ethanolamine kinase 1 | XM_017019580.1 | 37.4% | 34.8% | (many diffs) |
| 6 | human | 55500 | ETNK1 | ethanolamine kinase 1 | XR_002957349.1 | 6.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 579
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccaat tacatccacg tccctcccgg ctccccggag gtgcccaagc 121 tgaacgtcac cgttcaggat caggaggagc atcgctgccg ggagggggcc ctgagcctcc 181 tgcaacacct GCGGCCTCAC TGGGACCCCC AGGAGGTGAC CCTGCAGCTC TTCACAGATG 241 GAATCACAAA TAAACTTATT GGCTGTTACG TGGGAAACAC CATGGAGGAT GTAGTCCTGG 301 TGAGAATTTA TGGCAATAAG ACTGAGTTAT TAGTCGATCG AGATGAGGAA GTAAAGAGTT 361 TTCGAGTGTT GCAGGCTCAT GGGTGTGCAC CACAACTCTA CTGTACCTTC AATAATGGAC 421 TATGCTATGA ATTTATACAA GGAGAAGCAC TGGATCCAAA GCATGTCTGC AACCCAGCCA 481 TTTTCAGTTT ATCATCGTTG ACTCTTTGCA AAGGAAAAAC TACAAGATGT TTTGGATTAA 541 CCGGCTGCAG AGGGTCAAGG CTTCTGCTTA GTTTTTTCTG AGACCCAGCT TTCTTGTACA 601 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 661 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 721 AGGACGAATA AGCTCGGCGC CGATCATACC CACGCGTTAA GTCgacaatc aacctctgga 781 ttacaaaatt tgtgaaagat t