Construct: ORF TRCN0000489672

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019144.1_s317c1
DNA Barcode:
GCTATGCTTATTTCGCATCCGCTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LRRK1 (79705)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489672
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79705 LRRK1 leucine rich repeat kinase 1 XM_005254979.3 99.9% 99.9% 1332A>G;3930_3931insG
2 human 79705 LRRK1 leucine rich repeat kinase 1 XM_011522017.2 99.9% 99.9% 1332A>G;3930_3931insG
3 human 79705 LRRK1 leucine rich repeat kinase 1 XM_011522018.2 99.9% 99.9% 1332A>G;3930_3931insG
4 human 79705 LRRK1 leucine rich repeat kinase 1 XM_017022570.1 85.9% 85.9% 1_642del;1974A>G;4572_4573insG
5 human 79705 LRRK1 leucine rich repeat kinase 1 XM_011522015.3 74.1% 74% 1_1371del;2703A>G;5301_5302insG
6 human 79705 LRRK1 leucine rich repeat kinase 1 XM_011522016.2 68.4% 68.4% 1_1812del;3144A>G;5742_5743insG
7 human 79705 LRRK1 leucine rich repeat kinase 1 NM_024652.6 64.9% 64.9% 1_2115del;3447A>G;6045_6046insG
8 human 79705 LRRK1 leucine rich repeat kinase 1 XM_011522012.2 64.9% 64.9% 1_2115del;3447A>G;6045_6046insG
9 human 79705 LRRK1 leucine rich repeat kinase 1 XM_011522013.2 64.9% 64.9% 1_2115del;3447A>G;6045_6046insG
10 human 79705 LRRK1 leucine rich repeat kinase 1 XM_011522014.2 63.2% 63.2% (many diffs)
11 human 79705 LRRK1 leucine rich repeat kinase 1 XR_931905.2 42.4% (many diffs)
12 human 79705 LRRK1 leucine rich repeat kinase 1 XM_011522019.2 18.9% 17.5% 1_2115del;3132_3133ins164;3258_3259ins2624
13 mouse 233328 Lrrk1 leucine-rich repeat kinase 1 XM_006540841.1 86.5% 90.9% (many diffs)
14 mouse 233328 Lrrk1 leucine-rich repeat kinase 1 XM_006540840.3 76.7% 80.7% (many diffs)
15 mouse 233328 Lrrk1 leucine-rich repeat kinase 1 XM_006540839.3 56.7% 59.6% (many diffs)
16 mouse 233328 Lrrk1 leucine-rich repeat kinase 1 NM_146191.3 56.2% 59.1% (many diffs)
17 mouse 233328 Lrrk1 leucine-rich repeat kinase 1 XM_006540838.3 56.2% 59.1% (many diffs)
18 mouse 233328 Lrrk1 leucine-rich repeat kinase 1 XR_391340.3 43.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
4005
ORF length:
3933
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggccact gtcaaccagt gcttcttcac ggacaaggcc ctgtacgtgg
121tggtctggaa cctggcgctg ggggaggagg ccgtggccaa cctccagttc tggctgctca
181acatcgaggc caaggcccca aacgccgtgg tgctggtggt cgggacgcac ctggatttaa
241ttgaagccaa gttccgtgtg gaaaggattg caacgctgcg tgcctatgtg ctggcactct
301gccgctcccc ctccggctcc agggccacag gcttcccaga catcaccttc aaacacttac
361atgagatttc ctgcaagagc ctggaaggtc aggaagggct gcgacagctg attttccacg
421tcacgtgcag catgaaggac gtgggcagca ccatcggctg ccagcgactg gcagggcggc
481tgatccccag gagctacctg agcctgcagg aggccgtgct ggcagagcag cagcgccgca
541gccgggacga cgacgtgcag tacctgacgg acaggcagct ggagcagctg gtggagcaga
601cgcccgacaa cgacatcaag gactacgagg acctgcagtc agccatcagc ttcctcatag
661aaaccggcac cctgctccat ttcccggaca ccagccacgg cctgaggaac ctctacttcc
721tcgaccctat ttggctctcc gaatgtctgc agaggatctt taatattaag ggctctcggt
781cagtggccaa gaatggggtg atcagagcag aagacctcag gatgctgctg gtggggactg
841gcttcacgca gcagacggaa gagcagtact tccagttcct ggccaagttt gagatcgccc
901tgcccgtcgc caatgacagc tacctcctgc cccatctcct tccatctaaa cctggcctgg
961acacccacgg tatgcggcac cccacagcca acaccattca gagggtattt aagatgagct
1021tcgttcccgt tggcttctgg caaaggttta tagcacggat gctgatcagc ctggcggaga
1081tggacctgca gctttttgaa aacaagaaga atactaaaag caggaacagg aaagtcacca
1141tttacagttt tacaggaaac cagagaaatc gctgtagcac attcagagtg aaaagaaatc
1201agaccatcta ttggcaggaa gggctcctgg tcacttttga tgggggctac ctcagtgtgg
1261aatcttccga cgtgaactgg aaaaagaaga aaagcggagg aatgaaaatt gtttgccaat
1321cagaagtgag ggacttctca gccatggctt tcatcacgga ccacgtcaat tccttgattg
1381atcagtggtt tcccgccctg acggccacag agagcgacgg gacgccactc atggagcagt
1441acgtgccctg cccggtctgc gagacagcct gggcccagca cacggacccc agtgagaaat
1501cagaggatgt gcagtacttc gacatggaag actgtgtcct gacggccatc gagcgggact
1561tcatctcctg ccccagacac ccggacctcc ccgtgccgct gcaggagctg gtccctgaac
1621tgttcatgac cgacttcccg gccaggctct tcctggagaa cagcaagctg gagcacagcg
1681aggacgaggg cagcgtcctg ggccagggcg gcagtggcac cgtcatctac cgggcccggt
1741accagggcca gcctgtggcc gtcaagcgct tccacatcaa aaaattcaag aactttgcta
1801acgtaccggc agacaccatg ctgaggcacc tgcgggccac cgatgccatg aagaacttct
1861ccgagttccg gcaggaggcc agcatgctgc acgcgctgca gcacccctgc atcgtggcgc
1921tcatcggcat cagcatccac ccgctctgct tcgccctgga gctcgcgccg ctcagcagcc
1981tcaacaccgt gctgtccgag aacgccagag attcttcctt tatacccctg ggacacatgc
2041tcacccaaaa aatagcctac cagatcgcct cgggcctggc ctacctgcac aagaaaaaca
2101tcatcttctg tgacctgaag tcggacaaca ttctggtgtg gtcccttgac gtcaaggagc
2161acatcaacat caagctatct gactacggga tttcgaggca gtcattccat gagggcgccc
2221taggcgtgga gggcactcct ggctaccagg ccccagagat caggcctcgc attgtatatg
2281atgagaaggt agatatgttc tcctatggaa tggtgctcta cgagttgctg tcaggacagc
2341gccctgcact gggccaccac cagctccaga ttgccaagaa gctgtccaag ggcatccgcc
2401cggttctggg gcagccggag gaagtgcagt tccggcgact gcaggcgctc atgatggagt
2461gctgggacac taagccagag aagcgaccgc tggccctgtc ggtggtgagc cagatgaagg
2521acccgacttt tgccaccttc atgtatgaac tgtgctgtgg gaagcagaca gccttcttct
2581catcccaggg ccaggagtac accgtggtgt tttgggatgg aaaagaggag tccaggaact
2641acacggtggt gaacacagag aagggcctca tggaggtgca gaggatgtgc tgccctggga
2701tgaaggtgag ctgccagctc caggtccaga gatccctgtg gacagccacc gaggaccaga
2761aaatctacat ctacaccctc aagggcatgt gccccttaaa cacaccccaa caggccttgg
2821atactccagc tgtcgtcacc tgcttcttgg ccgtgcctgt tattaaaaag aattcctacc
2881tggtcttagc gggcctcgcc gatgggcttg tggctgtgtt tcccgtggtg cggggcaccc
2941caaaggacag ctgctcctac ctgtgctcac acacagccaa caggtccaag ttcagcatcg
3001cggatgaaga cgcacggcag aacccctacc cagtgaaggc catggaggtg gtcaacagcg
3061gctctgaggt ctggtacagc aatgggccgg gcctccttgt catcgactgt gcctccctgg
3121agatctgcag gcggctggag ccctacatgg ccccctccat ggttacgtca gtcgtgtgca
3181gctctgaggg cagaggggag gaggtcgtct ggtgcctgga tgacaaggcc aactccttgg
3241tgatgtacca ctccaccacc taccagctgt gtgcccggta cttctgcggg gtccccagcc
3301ccctcaggga catgtttccc gtgcggccct tggacacgga acccccggca gccagccaca
3361cggccaaccc aaaggtgcct gagggggact ccatcgcgga cgtgagcatc atgtacagtg
3421aggagctggg cacgcagatc ctgatccacc aggaatcact cactgactac tgctccatgt
3481cctcctactc ctcatcccca ccccgccagg ctgccaggtc cccctcaagc ctccccagct
3541ccccagcaag ttcttccagt gtgcctttct ccaccgactg cgaggactca gacatgctac
3601atacgcccgg tgctgcctcc gacaggtctg agcatgacct gacccccatg gacggggaga
3661ccttcagcca gcacctgcag gccgtgaaga tccTCGCCGT CAGAGACCTC ATTTGGGTCC
3721CCAGGCGCGG TGGAGATGTT ATCGTCATTG GCCTGGAGAA GGATTCTGGC GCCCAGCGGG
3781GCCGAGTCAT TGCCGTCTTA AAAGCCCGAG AGCTGACTCC GCATGGGGTG CTGGTGGATG
3841CTGCCGTGGT GGCAAAGGAC ACTGTTGTGT GCACCTTTGA AAATGAAAAC ACAGAGTGGT
3901GCCTGGCCGT CTGGAGGGGC TGGGGCGCCA GGGAGTTCGA CATTTTCTAC CAGTCCTACG
3961AGGAGCTGGG CCGGCTGGAG GCTTGCACTC GCAAGAGAAG GGACCCAGCT TTCTTGTACA
4021AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
4081AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
4141AGGACGAGCT ATGCTTATTT CGCATCCGCT TACGCGTTAA GTCgacaatc aacctctgga
4201ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)