Construct: ORF TRCN0000489689
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020265.1_s317c1
- DNA Barcode:
- AGCACTGAAGATCATTCCGTGCGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TK2 (7084)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489689
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7084 | TK2 | thymidine kinase 2 | NM_001172643.1 | 100% | 100% | |
| 2 | human | 7084 | TK2 | thymidine kinase 2 | NM_001271934.2 | 92% | 91% | (many diffs) |
| 3 | human | 7084 | TK2 | thymidine kinase 2 | NM_004614.5 | 85.4% | 85.2% | (many diffs) |
| 4 | human | 7084 | TK2 | thymidine kinase 2 | NM_001172645.1 | 78.7% | 78.4% | (many diffs) |
| 5 | human | 7084 | TK2 | thymidine kinase 2 | NM_001172644.2 | 76.1% | 75.8% | (many diffs) |
| 6 | human | 7084 | TK2 | thymidine kinase 2 | NM_001272050.2 | 71.7% | 71.7% | 0_1ins198 |
| 7 | human | 7084 | TK2 | thymidine kinase 2 | NM_001271935.1 | 62.3% | 49.5% | 355_356ins169;438_439ins95 |
| 8 | human | 7084 | TK2 | thymidine kinase 2 | NR_073520.2 | 11.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 774
- ORF length:
- 702
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggtgcg ttctgccagc gtcctagcag tgataaagaa caggaaaaag 121 agaaaaaatc agtgatctgt gtcgagggca atattgcaag tgggaagacg acatgcctgg 181 aattcttctc caacgcgaca gacgtcgagg tgttaacgga gcctgtgtcc aagtggagaa 241 atgtccgtgg ccacaatcct ctgggcctga tgtaccacga tgcctctcgc tggggtctta 301 cgctacagac ttatgtgcag ctcaccatgc tggacaggca tactcgtcct caggtgtcat 361 ctgtacggtt gatgGAGAGG TCGATTCACA GCGCAAGATA CATTTTTGTA GAAAACCTGT 421 ATAGAAGTGG GAAGATGCCA GAAGTGGACT ATGTAGTTCT GTCGGAATGG TTTGACTGGA 481 TCTTGAGGAA CATGGACGTG TCTGTTGATT TGATAGTTTA CCTTCGGACC AATCCTGAGA 541 CTTGTTACCA GAGGTTAAAG AAGAGATGCA GGGAAGAGGA GAAGGTCATT CCGCTGGAAT 601 ACCTGGAAGC AATTCACCAT CTCCATGAGG AGTGGCTCAT CAAAGGCAGC CTTTTCCCCA 661 TGGCAGCCCC TGTTCTGGTG ATTGAGGCTG ACCACCACAT GGAGAGGATG TTAGAACTCT 721 TTGAACAAAA TCGGGATCGA ATATTAACTC CAGAGAATCG GAAGCATTGC CCATGAGACC 781 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 841 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 901 ATATATCTTG TGGAAAGGAC GAAGCACTGA AGATCATTCC GTGCGTACGC GTTAAGTCga 961 caatcaacct ctggattaca aaatttgtga aagatt