Construct: ORF TRCN0000489713
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020310.1_s317c1
- DNA Barcode:
- AGGCAGTCTCACCTTTAGTACACT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAPKAPK2 (9261)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489713
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9261 | MAPKAPK2 | MAPK activated protein kina... | NM_004759.5 | 95.5% | 95.6% | 51G>T;66_113del |
| 2 | human | 9261 | MAPKAPK2 | MAPK activated protein kina... | XM_005273353.3 | 95.1% | 95.1% | (many diffs) |
| 3 | human | 9261 | MAPKAPK2 | MAPK activated protein kina... | NM_032960.4 | 86.1% | 84.1% | (many diffs) |
| 4 | human | 9261 | MAPKAPK2 | MAPK activated protein kina... | XM_017002810.1 | 69.9% | 67.8% | (many diffs) |
| 5 | mouse | 17164 | Mapkapk2 | MAP kinase-activated protei... | NM_008551.2 | 79.5% | 83.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1134
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgctgtcc aactcccagg gccagagccc gccggtgccg ttccccgccc 121 ctgccccgcc gccgcagccc ccgcagcagt tcccgcagtt ccacgtcaag tccggcctgc 181 agatcaagaa gaacgccatc atcgatgact acaaggtcac cagccaggtc ctggggctgg 241 gcatcaacgg caaagttttg cagatcttca acaagaggac ccaggagaaa ttcgccctca 301 aaatgcttca ggactgcccc aaggcccgca gggaggtgga gctgcactgg cgggcctccc 361 agtgcccgca catcgtacgg atcgtggatg tgtacgagaa tctgtacgca gggaggaagt 421 gcctgctgat tgtcatggaa tgtttggacg gtggagaact ctttagccga atccaggatc 481 gaggagacca ggcattcaca gaaagagaag catccgaaat catgaagagc atcggtgagg 541 ccatccagta tctgcattca atcaacattg cccatcggga tgtcaagcct gagaatctct 601 tatacacctc caaaaggccc aacgccatcc tgaaactcac tgactttggc tttgccaagg 661 aaaccaccag ccacaactct ttgaccactc cttgttatac accgtactat gtggctccag 721 aagtgctggg tccagagaag tatgacaagt cctgtgacat gtggtccctg ggtgtcatca 781 tgtacatcct gctgtgtggg tatcccccct tcTACTCCAA CCACGGCCTT GCCATCTCTC 841 CGGGCATGAA GACTCGCATC CGAATGGGCC AGTATGAATT TCCCAACCCA GAATGGTCAG 901 AAGTATCAGA GGAAGTGAAG ATGCTCATTC GGAATCTGCT GAAAACAGAG CCCACCCAGA 961 GAATGACCAT CACCGAGTTT ATGAACCACC CTTGGATCAT GCAATCAACA AAGGTCCCTC 1021 AAACCCCACT GCACACCAGC CGGGTCCTGA AGGAGGACAA GGAGCGGTGG GAGGATGTCA 1081 AGGGGTGTCT TCATGACAAG AACAGCGACC AGGCCACTTG GCTGACCAGG TTGTGAGACC 1141 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1201 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1261 ATATATCTTG TGGAAAGGAC GAAGGCAGTC TCACCTTTAG TACACTACGC GTTAAGTCga 1321 caatcaacct ctggattaca aaatttgtga aagatt