Construct: ORF TRCN0000489725
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021825.1_s317c1
- DNA Barcode:
- CCATTTGTTTAATCGGTTTCATTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- APP (351)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489725
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 351 | APP | amyloid beta precursor protein | NM_201414.3 | 100% | 100% | |
| 2 | human | 351 | APP | amyloid beta precursor protein | NM_001204303.2 | 97.4% | 97.4% | 1682_1683ins54 |
| 3 | human | 351 | APP | amyloid beta precursor protein | NM_001136131.2 | 94.9% | 94.9% | 0_1ins105 |
| 4 | human | 351 | APP | amyloid beta precursor protein | NM_201413.3 | 92.5% | 92.4% | 866_1033del |
| 5 | human | 351 | APP | amyloid beta precursor protein | NM_001136129.3 | 91.9% | 91.9% | 57_58ins168 |
| 6 | human | 351 | APP | amyloid beta precursor protein | NM_000484.4 | 90.2% | 90.1% | 866_1090del |
| 7 | human | 351 | APP | amyloid beta precursor protein | NM_001204302.2 | 90.1% | 90% | 866_1033del;1850_1851ins54 |
| 8 | human | 351 | APP | amyloid beta precursor protein | NM_001136016.3 | 89.8% | 90% | (many diffs) |
| 9 | human | 351 | APP | amyloid beta precursor protein | NM_001204301.2 | 87.9% | 87.7% | 866_1090del;1907_1908ins54 |
| 10 | human | 351 | APP | amyloid beta precursor protein | NM_001136130.3 | 82.9% | 82.8% | 57_58ins168;698_922del |
| 11 | human | 351 | APP | amyloid beta precursor protein | XM_024452075.1 | 65% | 64.7% | (many diffs) |
| 12 | mouse | 11820 | App | amyloid beta (A4) precursor... | NM_007471.3 | 89.2% | 97.4% | (many diffs) |
| 13 | mouse | 11820 | App | amyloid beta (A4) precursor... | XM_006522873.2 | 86.8% | 94.8% | (many diffs) |
| 14 | mouse | 11820 | App | amyloid beta (A4) precursor... | NM_001198824.1 | 82.6% | 90% | (many diffs) |
| 15 | mouse | 11820 | App | amyloid beta (A4) precursor... | NM_001198823.1 | 80.5% | 87.7% | (many diffs) |
| 16 | mouse | 11820 | App | amyloid beta (A4) precursor... | NM_001198825.1 | 80.3% | 87.6% | (many diffs) |
| 17 | mouse | 11820 | App | amyloid beta (A4) precursor... | NM_001198826.1 | 78.3% | 85.4% | (many diffs) |
| 18 | mouse | 11820 | App | amyloid beta (A4) precursor... | XM_006522874.1 | 58% | 62% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2154
- ORF length:
- 2085
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gctgcccggt ttggcactgc tcctgctggc cgcctggacg gctcgggcgc 121 tggaggtacc cactgatggt aatgctggcc tgctggctga accccagatt gccatgttct 181 gtggcagact gaacatgcac atgaatgtcc agaatgggaa gtgggattca gatccatcag 241 ggaccaaaac ctgcattgat accaaggaag gcatcctgca gtattgccaa gaagtctacc 301 ctgaactgca gatcaccaat gtggtagaag ccaaccaacc agtgaccatc cagaactggt 361 gcaagcgggg ccgcaagcag tgcaagaccc atccccactt tgtgattccc taccgctgct 421 tagttggtga gtttgtaagt gatgcccttc tcgttcctga caagtgcaaa ttcttacacc 481 aggagaggat ggatgtttgc gaaactcatc ttcactggca caccgtcgcc aaagagacat 541 gcagtgagaa gagtaccaac ttgcatgact acggcatgtt gctgccctgc ggaattgaca 601 agttccgagg ggtagagttt gtgtgttgcc cactggctga agaaagtgac aatgtggatt 661 ctgctgatgc ggaggaggat gactcggatg tctggtgggg cggagcagac acagactatg 721 cagatgggag tgaagacaaa gtagtagaag tagcagagga ggaagaagtg gctgaggtgg 781 aagaagaaga agccgatgat gacgaggacg atgaggatgg tgatgaggta gaggaagagg 841 ctgaggaacc ctacgaagaa gccacagaga gaaccaccag cattgccacc accaccacca 901 ccaccacaga gtctgtggaa gaggtggttc gagttcctac aacagcagcc agtacccctg 961 atgccgttga caagtatctc gagacacctg gggatgagaa tgaacatgcc catttccaga 1021 aagccaaaga gaggcttgag gccaagcacc gagagagaat gtcccaggtc atgagagaat 1081 gggaagaggc agaacgtcaa gcaaagaact tgcctaaagc tgataagaag gcagttatcc 1141 agcatttcca ggagaaagtg gaatctttgg aacaggaagc agccaacgag agacagcagc 1201 tggtggagac acacatggcc agagtggaag ccatgctcaa tgaccgccgc cgcctggccc 1261 tggagaacta catcaccgct ctgcaggctg ttcctcctcg gcctcgtcac gtgttcaata 1321 tgctaaagaa gtatgtccgc gcagaacaga aggacagaca gcacacccta aagcatttcg 1381 agcatgtgcg catggtggat cccaagaaag ccgctcagat ccggtcccag gttatgacac 1441 acctccgtgt gatttatgag cgcatgaatc agtctctctc cctgctctac aacgtgcctg 1501 cagtggccga ggagattcag gatgaagttg atgagctgct tcagaaagag caaaactatt 1561 cagatgacgt cttggccaac atgattagtg aaccaaggat cagttacgga aacgatgctc 1621 tcatgccatc tttgaccgaa acgaaaacca ccgtggagct ccttcccgtg aatggagagt 1681 tcagcctgga cgatctccag ccgtggcatt cttttggggc tgactctgtg ccagccaaca 1741 cagaaaacga agttgagcct gttgatgccc gccctgctgc cgaccgagga ctgaccactc 1801 GACCAGGTTC TGGGTTGACA AATATCAAGA CGGAGGAGAT CTCTGAAGTG AAGATGGATG 1861 CAGAATTCCG ACATGACTCA GGATATGAAG TTCATCATCA AAAATTGGTG TTCTTTGCAG 1921 AAGATGTGGG TTCAAACAAA GGTGCAATCA TTGGACTCAT GGTGGGCGGT GTTGTCATAG 1981 CGACAGTGAT CGTCATCACC TTGGTGATGC TGAAGAAGAA ACAGTACACA TCCATTCATC 2041 ATGGTGTGGT GGAGGTTGAC GCCGCTGTCA CCCCAGAGGA GCGCCACCTG TCCAAGATGC 2101 AGCAGAACGG CTACGAAAAT CCAACCTACA AGTTCTTTGA GCAGATGCAG AACTAGGACC 2161 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2221 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2281 ATATATCTTG TGGAAAGGAC GACCATTTGT TTAATCGGTT TCATTCACGC GTTAAGTCga 2341 caatcaacct ctggattaca aaatttgtga aagatt