Construct: ORF TRCN0000489738
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020683.1_s317c1
- DNA Barcode:
- ACGTTCGTAGTTTCTTTTAGTTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP4K5 (11183)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489738
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11183 | MAP4K5 | mitogen-activated protein k... | NM_006575.5 | 100% | 100% | |
| 2 | human | 11183 | MAP4K5 | mitogen-activated protein k... | NM_198794.3 | 100% | 100% | |
| 3 | human | 11183 | MAP4K5 | mitogen-activated protein k... | XM_006720013.4 | 100% | 100% | |
| 4 | human | 11183 | MAP4K5 | mitogen-activated protein k... | XM_024449461.1 | 100% | 100% | |
| 5 | human | 11183 | MAP4K5 | mitogen-activated protein k... | XM_011536377.3 | 99.8% | 99.8% | 1703_1704insAGA |
| 6 | human | 11183 | MAP4K5 | mitogen-activated protein k... | XM_011536378.3 | 92% | 92% | 0_1ins201 |
| 7 | human | 11183 | MAP4K5 | mitogen-activated protein k... | XM_011536379.3 | 83.9% | 83.9% | 0_1ins408 |
| 8 | human | 11183 | MAP4K5 | mitogen-activated protein k... | XM_011536380.3 | 63.5% | 59.3% | (many diffs) |
| 9 | mouse | 399510 | Map4k5 | mitogen-activated protein k... | NM_201519.2 | 89.3% | 96.1% | (many diffs) |
| 10 | mouse | 399510 | Map4k5 | mitogen-activated protein k... | XM_006516054.3 | 89.2% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2610
- ORF length:
- 2538
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaggcc ccgctgcggc ctgccgcgga catcctgagg cggaacccgc 121 agcaggacta cgaactcgtc cagagggtcg gcagcggcac ctacggggac gtctataagg 181 ccagaaatgt acacacagga gagctggctg cagtaaaaat cattaaattg gagcctggag 241 atgatttttc tttgattcaa caagaaatat ttatggttaa agaatgtaaa cattgtaaca 301 tcgttgccta ctttgggagt tatcttagtc gggaaaaact atggatttgt atggaatact 361 gtggtggcgg atcacttcaa gatatttacc atgttactgg accattatca gaattgcaaa 421 tagcctatgt atgcagagaa accttacagg gtcttgccta tttgcatact aaaggcaaaa 481 tgcatagaga tatcaaaggt gctaatattt tattgacaga ccatggcgat gtaaaattag 541 ctgactttgg tgtggctgca aaaataacag ctaccattgc aaaacgaaaa tctttcattg 601 gcacccctta ctggatggcc ccagaagttg cagcagtaga gaagaatggt ggctacaacc 661 aactctgtga tatctgggca gtaggaataa cagcaattga acttggagaa cttcagccac 721 ctatgtttga tctccaccca atgagggctc tcttcttaat gtcaaaaagt aattttcagc 781 ctccaaaact aaaggacaaa acaaaatggt catcaacatt ccataatttt gtcaaaatag 841 cactaaccaa aaacccaaaa aaaagaccaa ctgctgaaag acttctgact cacacttttg 901 ttgcacagcc aggtctctct agagccctag cagttgaact gttagacaaa gtgaacaatc 961 cagataacca cgcacattac actgaagcag atgacgatga ctttgagccc catgcaatca 1021 ttcgtcatac cattagatct acaaacagga atgccagagc tgaacggaca gcttcagaaa 1081 taaattttga caaattacaa tttgaacctc ctctgagaaa agaaacagaa gcacgagatg 1141 aaatgggatt gtcatcagac ccaaatttca tgttacagtg gaatcctttt gttgatggtg 1201 caaatactgg caaatcaacc tcaaaacgtg caataccacc tcccctacct cctaagccaa 1261 ggataagcag ttaccctgaa gacaactttc cggatgaaga aaaagcatca accataaaac 1321 attgtcctga ttcagaaagc agagctcccc aaattctcag aagacagagt agcccaagtt 1381 gtgggcctgt ggcagagact tcttctattg gaaatggtga tggtatttca aaactgatga 1441 gtgaaaatac agaaggatca gcacaagcac cacagttacc acgaaaaaag gacaaacgag 1501 acttccctaa accagccatc aatggccttc cacccacccc aaaagttctg atgggagcat 1561 gcttttcaaa agtttttgat ggctgtcctt tgaaaattaa ttgtgcaaca tcctggatac 1621 atcctgatac aaaagatcag tacattattt ttggaactga agatggtatt tacacactga 1681 atctcaatga gctacatgag gcaacgatgg aacagttatt tccacggaag tgtacttggc 1741 tgtatgttat caataatact ttaatgtcat tatcagaagg aaaaaccttt cagctctact 1801 ctcacaatct tatagctttg tttgaacatg ccaaaaaacc aggattagct gcccatattc 1861 aaactcacag gtttccagac cgaatactac caagaaaatt cgctttaaca acaaagattc 1921 ctgatacaaa aggctgccac aaatgttgca tagtcagaaa cccttacacg ggacataaat 1981 acctctgtgg agctttacag tctggaattg ttttacttca gtggtatgag ccaatgcaga 2041 aattcatgtt gataaagcac tttgattttc ctttgccaag tcctttgaat gtttttgaaa 2101 tgctggtgat acctgaacag gaatacccta tggtctgtgt agctattagc aaaggcactg 2161 aatcgaatca ggtagttcag tttgagacaa tcaatttgaa ctctgcatct tcatGGTTTA 2221 CAGAAATTGG TGCAGGCAGC CAGCAGTTAG ATTCCATTCA TGTAACACAG TTGGAGAGAG 2281 ATACCGTTTT AGTGTGTTTA GACAAATTTG TGAAAATTGT AAATCTACAA GGAAAATTAA 2341 AATCAAGTAA GAAACTGGCC TCTGAGTTAA GTTTTGATTT TCGCATTGAA TCTGTAGTAT 2401 GCCTTCAAGA CAGTGTGTTG GCTTTCTGGA AACATGGGAT GCAGGGTAAA AGCTTCAAGT 2461 CAGATGAGGT TACCCAGGAG ATTTCAGATG AAACAAGAGT TTTCCGCTTA TTAGGATCAG 2521 ACAGGGTTGT CGTTTTGGAA AGTAGGCCAA CAGAAAATCC TACTGCACAC AGCAATCTCT 2581 ACATCTTGGC TGGACATGAA AATAGTTACT AGAACCCAGC TTTCTTGTAC AAAGTGGTTG 2641 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2701 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC 2761 GTTCGTAGTT TCTTTTAGTT TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 2821 ttgtgaaaga tt