Construct: ORF TRCN0000489739

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020387.1_s317c1
DNA Barcode:
AGAACCGGCTTCTTCCAGTACGTG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
SRPK2 (6733)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489739
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6733 SRPK2 SRSF protein kinase 2 NM_001278273.1 99.9% 99.8% 1117A>G
2 human 6733 SRPK2 SRSF protein kinase 2 NM_001350738.1 99.9% 99.8% 1117A>G
3 human 6733 SRPK2 SRSF protein kinase 2 NM_182691.2 99.9% 99.8% 1117A>G
4 human 6733 SRPK2 SRSF protein kinase 2 NM_001350739.1 97.9% 98.4% (many diffs)
5 human 6733 SRPK2 SRSF protein kinase 2 NM_182692.3 97.4% 96.5% (many diffs)
6 human 6733 SRPK2 SRSF protein kinase 2 NM_001350743.1 95.6% 95.5% 1117A>G;1881_1973del
7 human 6733 SRPK2 SRSF protein kinase 2 NM_001350744.1 95.6% 95.5% 1117A>G;1881_1973del
8 human 6733 SRPK2 SRSF protein kinase 2 XM_006716098.3 95.6% 95.5% 1117A>G;1881_1973del
9 human 6733 SRPK2 SRSF protein kinase 2 XM_011516538.3 95.6% 95.5% 1117A>G;1881_1973del
10 human 6733 SRPK2 SRSF protein kinase 2 NM_001350742.1 94.8% 94.7% 1_111del;1228A>G
11 human 6733 SRPK2 SRSF protein kinase 2 NM_001350741.1 93.3% 92.4% (many diffs)
12 human 6733 SRPK2 SRSF protein kinase 2 NM_001350745.1 93.2% 93% 616_617ins138;979A>G
13 human 6733 SRPK2 SRSF protein kinase 2 XM_011516536.3 93% 92.6% (many diffs)
14 human 6733 SRPK2 SRSF protein kinase 2 XM_017012561.2 92.9% 93.3% (many diffs)
15 human 6733 SRPK2 SRSF protein kinase 2 NM_001350740.1 92.4% 91.7% (many diffs)
16 human 6733 SRPK2 SRSF protein kinase 2 XM_024446893.1 90.9% 90.8% 1_111del;1228A>G;1992_2084del
17 human 6733 SRPK2 SRSF protein kinase 2 XM_024446895.1 90.6% 90.3% (many diffs)
18 human 6733 SRPK2 SRSF protein kinase 2 XM_024446894.1 88.7% 88% (many diffs)
19 human 6733 SRPK2 SRSF protein kinase 2 NM_001350746.1 80% 78.1% (many diffs)
20 human 6733 SRPK2 SRSF protein kinase 2 XR_001744860.2 60.5% (many diffs)
21 human 6733 SRPK2 SRSF protein kinase 2 XR_001744863.2 49.6% 1_173del;1290A>G;2238_4151del
22 human 6733 SRPK2 SRSF protein kinase 2 XR_001744861.2 48.5% (many diffs)
23 human 6733 SRPK2 SRSF protein kinase 2 XR_001744862.2 45.6% (many diffs)
24 human 6733 SRPK2 SRSF protein kinase 2 XR_001744859.2 43.7% (many diffs)
25 mouse 20817 Srpk2 serine/arginine-rich protei... NM_009274.2 89.8% 94.1% (many diffs)
26 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320769.1 89.8% 94.1% (many diffs)
27 mouse 20817 Srpk2 serine/arginine-rich protei... XM_006535661.3 87.3% 91% (many diffs)
28 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249755.2 86% 90.1% (many diffs)
29 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249756.2 86% 90.1% (many diffs)
30 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249757.2 86% 90.1% (many diffs)
31 mouse 20817 Srpk2 serine/arginine-rich protei... XM_006535660.3 85.3% 89.3% (many diffs)
32 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320767.1 84.1% 88.1% (many diffs)
33 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249753.2 83.6% 87.1% (many diffs)
34 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249754.2 83.6% 88% (many diffs)
35 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320768.1 82.4% 86.7% (many diffs)
36 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249751.2 81.8% 85.7% (many diffs)
37 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249752.2 80.7% 84.5% (many diffs)
38 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320771.1 76.5% 80.1% (many diffs)
39 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249758.2 73.2% 76.6% (many diffs)
40 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320770.1 73.2% 76.6% (many diffs)
41 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320774.1 69.3% 70.3% (many diffs)
42 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320776.1 68.1% 69% (many diffs)
43 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320775.1 68% 70.9% (many diffs)
44 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320777.1 66.8% 69.6% (many diffs)
45 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320773.1 66.3% 67.3% (many diffs)
46 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320772.1 65.2% 66.1% (many diffs)
47 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249759.2 65.1% 67.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2136
ORF length:
2064
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgtcagtt aactctgaga agtcgtcctc ttcagaaagg ccggagcctc
121aacagaaagc tcctttagtt cctcctcctc caccgccacc accaccacca ccgccacctt
181tgccagaccc cacacccccg gagccagagg aggagatcct gggatcagat gatgaggagc
241aagaggaccc tgcggactac tgcaaaggtg gatatcatcc agtgaaaatt ggagacctct
301tcaatggccg gtatcatgtt attagaaagc ttggatgggg gcacttctct actgtctggc
361tgtgctggga tatgcagggg aaaagatttg ttgcaatgaa agttgtaaaa agtgcccagc
421attatacgga gacagccttg gatgaaataa aattgctcaa atgtgttcga gaaagtgatc
481ccagtgaccc aaacaaagac atggtggtcc agctcattga cgacttcaag atttcaggca
541tgaatgggat acatgtctgc atggtcttcg aagtacttgg ccaccatctc ctcaagtgga
601tcatcaaatc caactatcaa ggcctcccag tacgttgtgt gaagagtatc attcgacagg
661tccttcaagg gttagattac ttacacagta agtgcaagat cattcatact gacataaagc
721cggaaaatat cttgatgtgt gtggatgatg catatgtgag aagaatggca gctgaggcca
781ctgagtggca gaaagcaggt gctcctcctc cttcagggtc tgcagtgagt acggctccac
841agcagaaacc tataggaaaa atatctaaaa acaaaaagaa aaaactgaaa aagaaacaga
901agaggcaggc tgagttattg gagaagcgcc tgcaggagat agaagaattg gagcgagaag
961ctgaaaggaa aataatagaa gaaaacatca cctcagctgc accttccaat gaccaggatg
1021gcgaatactg cccagaggtg aaactaaaaa caacaggatt agaggaggcg gctgaggcag
1081agactgcaaa ggacaatggt gaagctgagg accaggaaga gaaagaagat gctgagaaag
1141aaaacattga aaaagatgaa gatgatgtag atcaggaact tgcgaacgta gaccctacgt
1201ggatagaatc acctaaaacc aatggccata ttgagaatgg cccattctca ctggagcagc
1261aactggacga tgaagatgat gatgaagaag actgcccaaa tcctgaggaa tataatcttg
1321atgagccaaa tgcagaaagt gattacacat atagcagctc ctatgaacaa ttcaatggtg
1381aattgccaaa tggacgacat aaaattcccg agtcacagtt cccagagttt tccacctcgt
1441tgttctctgg atccttagaa cctgtggcct gcggctctgt gctttctgag ggatcaccac
1501ttactgagca agaggagagc agtccatccc atgacagaag cagaacggtt tcagcctcca
1561gtactgggga tttgccaaaa gcaaaaaccc gggcagctga cttgttggtg aatcccctgg
1621atccgcggaa tgcagataaa attagagtaa aaattgctga cctgggaaat gcttgttggg
1681tgcataaaca cttcacggaa GACATCCAGA CGCGTCAGTA CCGCTCCATA GAGGTTTTAA
1741TAGGAGCGGG GTACAGCACC CCTGCGGACA TCTGGAGCAC GGCGTGTATG GCATTTGAGC
1801TGGCAACGGG AGATTATTTG TTTGAACCAC ATTCTGGGGA AGACTATTCC AGAGACGAAG
1861ACCACATAGC CCACATCATA GAGCTGCTAG GCAGTATTCC AAGGCACTTT GCTCTATCTG
1921GAAAATATTC TCGGGAATTC TTCAATCGCA GAGGAGAACT GCGACACATC ACCAAGCTGA
1981AGCCCTGGAG CCTCTTTGAT GTACTTGTGG AAAAGTATGG CTGGCCCCAT GAAGATGCTG
2041CACAGTTTAC AGATTTCCTG ATCCCGATGT TAGAAATGGT TCCAGAAAAA CGAGCCTCAG
2101CTGGCGAATG CCTTCGGCAT CCTTGGTTGA ATTCTTAGGA CCCAGCTTTC TTGTACAAAG
2161TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
2221GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
2281ACGAAGAACC GGCTTCTTCC AGTACGTGAC GCGTTAAGTC gacaatcaac ctctggatta
2341caaaatttgt gaaagatt

Download FASTA (ORF) (Full)