Construct: ORF TRCN0000489748
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019524.1_s317c1
- DNA Barcode:
- CTCCGACCCGTGTTGTGCATATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTGFR (5737)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489748
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5737 | PTGFR | prostaglandin F receptor | NM_000959.4 | 99.9% | 99.7% | 1077_1078insG |
| 2 | human | 5737 | PTGFR | prostaglandin F receptor | NM_001039585.2 | 81.4% | 75.5% | (many diffs) |
| 3 | human | 5737 | PTGFR | prostaglandin F receptor | XM_017001873.1 | 80% | 75.2% | (many diffs) |
| 4 | human | 5737 | PTGFR | prostaglandin F receptor | XR_001737338.1 | 57% | 1_335del;1134_1274del;1363_1364ins191 | |
| 5 | human | 5737 | PTGFR | prostaglandin F receptor | XR_946715.2 | 53.5% | (many diffs) | |
| 6 | mouse | 19220 | Ptgfr | prostaglandin F receptor | NM_008966.3 | 84.1% | 87.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1155
- ORF length:
- 1080
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgtcc atgaacaatt ccaaacagct agtgtctcct gcagctgcgc 121 ttctttcaaa cacaacctgc cagacggaaa accggctttc cgtatttttt tcagtaatct 181 tcatgacagt gggaatcttg tcaaacagcc ttgccatcgc cattctcatg aaggcatatc 241 agagatttag acagaagtcc aaggcatcgt ttctgctttt ggccagtggc ctggtaatca 301 ctgatttctt tggccatctc atcaatggag ccatagcagt atttgtatat gcttctgata 361 aagaatggat ccgctttgac caatcaaatg tcctttgcag tatttttggt atctgcatgg 421 tgttttctgg tctgtgccca cttcttctag gcagtgtgat ggccattgag cggtgtattg 481 gagtcacaaa accaatattt cattctacga aaattacatc caaacatgtg aaaatgatgt 541 taagtggtgt gtgcttgttt gctgttttca tagctttgct gcccatcctt ggacatcgag 601 actataaaat tcaggcgtcg aggacctggt gtttctacaa cacagaagac atcaaagact 661 gggaagatag attttatctt ctactttttt cttttctggg gctcttagcc cttGGTGTTT 721 CATTGTTGTG CAATGCAATC ACAGGAATTA CACTTTTAAG AGTTAAATTT AAAAGTCAGC 781 AGCACAGACA AGGCAGATCT CATCATTTGG AAATGGTAAT CCAGCTCCTG GCGATAATGT 841 GTGTCTCCTG TATTTGTTGG AGCCCATTTC TGGTTACAAT GGCCAACATT GGAATAAATG 901 GAAATCATTC TCTGGAAACC TGTGAAACAA CACTTTTTGC TCTCCGAATG GCAACATGGA 961 ATCAAATCTT AGATCCTTGG GTATATATTC TTCTACGAAA GGCTGTCCTT AAGAATCTCT 1021 ATAAGCTTGC CAGTCAATGC TGTGGAGTGC ATGTCATCAG CTTACATATT TGGGAGCTTA 1081 GTTCCATTAA AAATTCCTTA AAGGTTGCTG CTATTTCTGA GTCACCAGTT GCAGAGAAAT 1141 CAGCAAGCAC CGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1201 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1261 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACTC CGACCCGTGT TGTGCATATA 1321 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t