Construct: ORF TRCN0000489759
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020421.1_s317c1
- DNA Barcode:
- TAGACGATTGGGTTGGAAGCTCTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LIMK1 (3984)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489759
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3984 | LIMK1 | LIM domain kinase 1 | NM_002314.4 | 100% | 100% | |
| 2 | human | 3984 | LIMK1 | LIM domain kinase 1 | NM_001204426.2 | 93.8% | 92.8% | (many diffs) |
| 3 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | NM_010717.3 | 87.9% | 95.2% | (many diffs) |
| 4 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | NM_001305875.1 | 82.6% | 84.8% | (many diffs) |
| 5 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | XM_006504396.3 | 72.7% | 68.5% | (many diffs) |
| 6 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | NR_131247.1 | 43.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 84
- ORF end:
- 2025
- ORF length:
- 1941
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggcccccttc accatgaggt tgacgctact ttgttgcacc tggagggaag 121 aacgtatggg agaggaagga agcgagttgc ccgtgtgtgc aagctgcggc cagaggatct 181 atgatggcca gtacctccag gccctgaacg cggactggca cgcagactgc ttcaggtgtt 241 gtgactgcag tgcctccctg tcgcaccagt actatgagaa ggatgggcag ctcttctgca 301 agaaggacta ctgggcccgc tatggcgagt cctgccatgg gtgctctgag caaatcacca 361 agggactggt tatggtggct ggggagctga agtaccaccc cgagtgtttc atctgcctca 421 cgtgtgggac ctttatcggt gacggggaca cctacacgct ggtggagcac tccaagctgt 481 actgcgggca ctgctactac cagactgtgg tgacccccgt catcgagcag atcctgcctg 541 actcccctgg ctcccacctg ccccacaccg tcaccctggt gtccatccca gcctcatctc 601 atggcaagcg tggactttca gtctccattg accccccgca cggcccaccg ggctgtggca 661 ccgagcactc acacaccgtc cgcgtccagg gagtggatcc gggctgcatg agcccagatg 721 tgaagaattc catccacgtc ggagaccgga tcttggaaat caatggcacg cccatccgaa 781 atgtgcccct ggacgagatt gacctgctga ttcaggaaac cagccgcctg ctccagctga 841 ccctcgagca tgaccctcac gatacactgg gccacgggct ggggcctgag accagccccc 901 tgagctctcc ggcttatact cccagcgggg aggcgggcag ctctgcccgg cagaaacctg 961 tcttgaggag ctgcagcatc gacaggtctc cgggcgctgg ctcactgggc tccccggcct 1021 cccagcgcaa ggacctgggt cgctctgagt ccctccgcgt agtctgccgg ccacaccgca 1081 tcttccggcc gtcggacctc atccacgggg aggtgctggg caagggctgc ttcggccagg 1141 ctatcaaggt gacacaccgt gagacaggtg aggtgatggt gatgaaggag ctgatccggt 1201 tcgacgagga gacccagagg acgttcctca aggaggtgaa ggtcatgcga tgcctggaac 1261 accccaacgt gctcaagttc atcggggtgc tctacaagga caagaggctc aacttcatca 1321 ctgagtacat caagggcggc acgctccggg gcatcatcaa gagcatggac agccagtacc 1381 catggagcca gagagtgagc tttgccaagg acatcgcatc agggatggcc tacctccact 1441 ccatgaacat catccaccga gacctcaact cccacaactg cctggtccgc gagaacaaga 1501 atgtggtggt ggctgacttc gggctggcgc gtctcatggt ggacgagaag actcagcctg 1561 agggcctgcg gagcctcaag aagccagacc gcaagaagcg ctacaccgtg gtgggcaacc 1621 cctactggat ggcacctGAG ATGATCAACG GCCGCAGCTA TGATGAGAAG GTGGATGTGT 1681 TCTCCTTTGG GATCGTCCTG TGCGAGATCA TCGGGCGGGT GAACGCAGAC CCTGACTACC 1741 TGCCCCGCAC CATGGACTTT GGCCTCAACG TGCGAGGATT CCTGGACCGC TACTGCCCCC 1801 CAAACTGCCC CCCGAGCTTC TTCCCCATCA CCGTGCGCTG TTGCGATCTG GACCCCGAGA 1861 AGAGGCCATC CTTTGTGAAG CTGGAACACT GGCTGGAGAC CCTCCGCATG CACCTGGCCG 1921 GCCACCTGCC ACTGGGCCCA CAGCTGGAGC AGCTGGACAG AGGTTTCTGG GAGACCTACC 1981 GGCGCGGCGA GAGCGGACTG CCTGCCCACC CTGAGGTCCC CGACTGAAAG CTTAAGGGTG 2041 GGCGCGCCGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2101 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2161 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATAGACG ATTGGGTTGG AAGCTCTGAC 2221 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt