Construct: ORF TRCN0000489779
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020537.1_s317c1
- DNA Barcode:
- GCCGATCGCGCTTCGCGCGTGTAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MATK (4145)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489779
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4145 | MATK | megakaryocyte-associated ty... | NM_139355.3 | 100% | 100% | |
| 2 | human | 4145 | MATK | megakaryocyte-associated ty... | XM_011528018.1 | 100% | 100% | |
| 3 | human | 4145 | MATK | megakaryocyte-associated ty... | NM_002378.4 | 95.2% | 94% | (many diffs) |
| 4 | human | 4145 | MATK | megakaryocyte-associated ty... | NM_139354.3 | 91.9% | 91.9% | 0_1ins123 |
| 5 | mouse | 17179 | Matk | megakaryocyte-associated ty... | XM_006513294.3 | 83.8% | 87.3% | (many diffs) |
| 6 | mouse | 17179 | Matk | megakaryocyte-associated ty... | NM_010768.2 | 83.7% | 87.3% | (many diffs) |
| 7 | mouse | 17179 | Matk | megakaryocyte-associated ty... | XM_006513295.3 | 83.7% | 87.3% | (many diffs) |
| 8 | mouse | 17179 | Matk | megakaryocyte-associated ty... | XM_011243376.2 | 81.1% | 85% | (many diffs) |
| 9 | mouse | 17179 | Matk | megakaryocyte-associated ty... | XM_011243377.2 | 81.1% | 85% | (many diffs) |
| 10 | mouse | 17179 | Matk | megakaryocyte-associated ty... | NM_001285853.1 | 77.8% | 82.4% | (many diffs) |
| 11 | mouse | 17179 | Matk | megakaryocyte-associated ty... | XM_006513298.3 | 77.8% | 82.4% | (many diffs) |
| 12 | mouse | 17179 | Matk | megakaryocyte-associated ty... | XM_006513300.2 | 77.8% | 82.4% | (many diffs) |
| 13 | mouse | 17179 | Matk | megakaryocyte-associated ty... | NM_001285854.1 | 77.8% | 82.4% | (many diffs) |
| 14 | mouse | 17179 | Matk | megakaryocyte-associated ty... | NM_001285855.1 | 77.8% | 82.4% | (many diffs) |
| 15 | mouse | 17179 | Matk | megakaryocyte-associated ty... | XM_006513299.2 | 77.8% | 82.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1593
- ORF length:
- 1521
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcgggg cgaggctctc tggtttcctg gcgggcattt cacggctgtg 121 attctgctga ggaacttccc cgggtgagcc cccgcttcct ccgagcctgg cacccccctc 181 ccgtctcagc caggatgcca acgaggcgct gggccccggg cacccagtgt atcaccaaat 241 gcgagcacac ccgccccaag ccaggggagc tggccttccg caagggcgac gtggtcacca 301 tcctggaggc ctgcgagaac aagagctggt accgcgtcaa gcaccacacc agtggacagg 361 aggggctgct ggcagctggg gcgctgcggg agcgggaggc cctctccgca gaccccaagc 421 tcagcctcat gccgtggttc cacgggaaga tctcgggcca ggaggctgtc cagcagctgc 481 agcctcccga ggatgggctg ttcctggtgc gggagtccgc gcgccacccc ggcgactacg 541 tcctgtgcgt gagctttggc cgcgacgtca tccactaccg cgtgctgcac cgcgacggcc 601 acctcacaat cgatgaggcc gtgttcttct gcaacctcat ggacatggtg gagcattaca 661 gcaaggacaa gggcgctatc tgcaccaagc tggtgagacc aaagcggaaa cacgggacca 721 agtcggccga ggaggagctg gccagggcgg gctggttact gaacctgcag catttgacat 781 tgggagcaca gatcggagag ggagagtttg gagctgtcct gcagggtgag tacctggggc 841 aaaaggtggc cgtgaagaat atcaagtgtg atgtgacagc ccaggccttc ctggacgaga 901 cggccgtcat gacgaagatg caacacgaga acctggtgcg tctcctgggc gtgatcctgc 961 accaggggct gtacattgtc atggagcacg tgagcaaggg caacctggtg aactttctgc 1021 ggacccgggg tcgagccctc gtgaacaccg ctcagctcct gcagttttct ctgcacgtgg 1081 ccgagggcat ggagtacctg gagagcaaga agcttgtgca ccgcgacctg gccgcccgca 1141 acatcctggt ctcagaggac ctggtggcca aggtcagcga ctttggcctg gCCAAAGCCG 1201 AGCGGAAGGG GCTAGACTCA AGCCGGCTGC CCGTCAAGTG GACGGCGCCC GAGGCTCTCA 1261 AACACGGGAA GTTCACCAGC AAGTCGGATG TCTGGAGTTT TGGGGTGCTG CTCTGGGAGG 1321 TCTTCTCATA TGGACGGGCT CCGTACCCTA AAATGTCACT GAAAGAGGTG TCGGAGGCCG 1381 TGGAGAAGGG GTACCGCATG GAACCCCCCG AGGGCTGTCC AGGCCCCGTG CACGTCCTCA 1441 TGAGCAGCTG CTGGGAGGCA GAGCCCGCCC GCCGGCCACC CTTCCGCAAA CTGGCCGAGA 1501 AGCTGGCCCG GGAGCTACGC AGTGCAGGTG CCCCAGCCTC CGTCTCAGGG CAGGACGCCG 1561 ACGGCTCCAC CTCGCCCCGA AGCCAGGAGC CCTAGAACCC AGCTTTCTTG TACAAAGTGG 1621 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1681 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1741 AGCCGATCGC GCTTCGCGCG TGTACACGCG TTAAGTCgac aatcaacctc tggattacaa 1801 aatttgtgaa agatt