Construct: ORF TRCN0000489780
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020568.1_s317c1
- DNA Barcode:
- TATTACATAAACCATTCCCATTTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DAPK1 (1612)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489780
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1612 | DAPK1 | death associated protein ki... | NM_001288729.1 | 99.9% | 99.9% | 3150C>T;3597C>T;4037G>A |
| 2 | human | 1612 | DAPK1 | death associated protein ki... | NM_001288730.2 | 99.9% | 99.9% | 3150C>T;3597C>T;4037G>A |
| 3 | human | 1612 | DAPK1 | death associated protein ki... | NM_001288731.2 | 99.9% | 99.9% | 3150C>T;3597C>T;4037G>A |
| 4 | human | 1612 | DAPK1 | death associated protein ki... | NM_004938.4 | 99.9% | 99.9% | 3150C>T;3597C>T;4037G>A |
| 5 | human | 1612 | DAPK1 | death associated protein ki... | XM_017014360.1 | 69.9% | 69.2% | (many diffs) |
| 6 | human | 1612 | DAPK1 | death associated protein ki... | XM_005251757.4 | 57.8% | 56.8% | (many diffs) |
| 7 | mouse | 69635 | Dapk1 | death associated protein ki... | NM_029653.3 | 87.7% | 95.1% | (many diffs) |
| 8 | mouse | 69635 | Dapk1 | death associated protein ki... | NM_134062.2 | 87.7% | 95.1% | (many diffs) |
| 9 | mouse | 69635 | Dapk1 | death associated protein ki... | NM_001285917.1 | 87% | 94.3% | (many diffs) |
| 10 | mouse | 69635 | Dapk1 | death associated protein ki... | XM_006517370.3 | 87% | 94.3% | (many diffs) |
| 11 | mouse | 69635 | Dapk1 | death associated protein ki... | XM_006517371.3 | 86.4% | 93.7% | (many diffs) |
| 12 | mouse | 69635 | Dapk1 | death associated protein ki... | XM_017315598.1 | 85.7% | 92.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 84
- ORF end:
- 4374
- ORF length:
- 4290
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggcccccttc accatgaccg tgttcaggca ggaaaacgtg gatgattact 121 acgacaccgg cgaggaactt ggcagtggac agtttgcggt tgtgaagaaa tgccgtgaga 181 aaagcaccgg cctccagtat gccgccaaat tcatcaagaa aaggaggact aagtccagcc 241 ggcggggtgt gagccgcgag gacatcgagc gggaggtcag catcctgaag gagatccagc 301 accccaatgt catcaccctg cacgaggtct atgagaacaa gacggacgtc atcctgatct 361 tggaactcgt tgcaggtggc gagctgtttg acttcttagc tgaaaaggaa tctttaactg 421 aagaggaagc aactgaattt ctcaaacaaa ttcttaatgg tgtttactac ctgcactccc 481 ttcaaatcgc ccactttgat cttaagcctg agaacataat gcttttggat agaaatgtcc 541 ccaaacctcg gatcaagatc attgactttg ggttggccca taaaattgac tttggaaatg 601 aatttaaaaa catatttggg actccagagt ttgtcgctcc tgagatagtc aactatgaac 661 ctcttggtct tgaggcagat atgtggagta tcggggtaat aacctatatc ctcctaagtg 721 gggcctcccc atttcttgga gacactaagc aagaaacgtt agcaaatgta tccgctgtca 781 actacgaatt tgaggatgaa tacttcagta ataccagtgc cctagccaaa gatttcataa 841 gaagacttct ggtcaaggat ccaaagaaga gaatgacaat tcaagatagt ttgcagcatc 901 cctggatcaa gcctaaagat acacaacagg cacttagtag aaaagcatca gcagtaaaca 961 tggagaaatt caagaagttt gcagcccgga aaaaatggaa acaatccgtt cgcttgatat 1021 cactgtgcca aagattatcc aggtcattcc tgtccagaag taacatgagt gttgccagaa 1081 gcgatgatac tctggatgag gaagactcct ttgtgatgaa agccatcatc catgccatca 1141 acgatgacaa tgtcccaggc ctgcagcacc ttctgggctc attatccaac tatgatgtta 1201 accaacccaa caagcacggg acacctccat tactcattgc tgctggctgt gggaatattc 1261 aaatactaca gttgctcatt aaaagaggct cgagaatcga tgtccaggat aagggcgggt 1321 ccaatgccgt ctactgggct gctcggcatg gccacgtcga taccttgaaa tttctcagtg 1381 agaacaaatg ccctttggat gtgaaagaca agtctggaga gatggccctc cacgtggcag 1441 ctcgctatgg ccatgctgac gtggctcagt tactgtgcag cttcggctca aatcccaata 1501 tccaggacaa ggaagaagaa acccccctgc actgtgctgc ttggcacggc tattactctg 1561 tggccaaagc cctttgtgaa gccggctgta acgtgaacat caagaaccga gaaggagaga 1621 cgcccctcct gacagcctct gccaggggct accacgacat cgtggagtgt ctggccgaac 1681 atggagccga ccttaatgct tgcgacaagg acggacacat tgcccttcat ctggctgtaa 1741 gacggtgtca gatggaggta atcaagactc tcctcagcca agggtgtttc gtcgattatc 1801 aagacaggca cggcaatact cccctccatg tggcatgtaa agatggcaac atgcctatcg 1861 tggtggccct ctgtgaagca aactgcaatt tggacatctc caacaagtat gggcgaacgc 1921 ctctgcacct tgcggccaac aacggaatcc tagacgtggt ccggtatctc tgtctgatgg 1981 gagccagcgt tgaggcgctg accacggacg gaaagacggc agaagatctt gctagatcgg 2041 aacagcacga gcacgtagca ggtctccttg caagacttcg aaaggatacg caccgaggac 2101 tcttcatcca gcagctccga cccacacaga acctgcagcc aagaattaag ctcaagctgt 2161 ttggccactc gggatccggg aaaaccaccc ttgtagaatc tctcaagtgt gggctgctga 2221 ggagcttttt cagaaggcgt cggcccagac tgtcttccac caactccagc aggttcccac 2281 cttcacccct ggcttctaag cccacagtct cagtgagcat caacaacctg tacccaggct 2341 gcgagaacgt gagtgtgagg agccgcagca tgatgttcga gccgggtctt accaaaggga 2401 tgctggaggt gtttgtggcc ccgacccacc acccgcactg ctcggccgat gaccagtcca 2461 ccaaggccat cgacatccag aacgcttatt tgaatggagt tggcgatttc agcgtgtggg 2521 agttctctgg aaatcctgtg tatttctgct gttatgacta ttttgctgca aatgatccca 2581 cgtcaatcca tgttgttgtc tttagtctag aagagcccta tgagatccag ctgaaccaag 2641 tgattttctg gctcagtttc ctgaagtccc ttgtcccagt tgaagaaccc atagccttcg 2701 gtggcaagct gaagaaccca ctccaagttg tcctggtggc cacccacgct gacatcatga 2761 atgttcctcg accggctgga ggcgagtttg gatatgacaa agacacatcg ttgctgaaag 2821 agattaggaa caggtttgga aatgatcttc acatttcaaa taagctgttt gttctggatg 2881 ctggggcttc tgggtcaaag gacatgaagg tacttcgaaa tcatctgcaa gaaatacgaa 2941 gccagattgt ttcggtctgt cctcccatga ctcacctgtg tgagaaaatc atctccacgc 3001 tgccttcctg gaggaagctc aatggaccca accagctgat gtcgctgcag cagtttgtgt 3061 acgacgtgca ggaccagctg aaccccctgg ccagcgagga ggacctcagg cgcattgctc 3121 agcagctcca cagcacaggc gagatcaaca tcatgcaaag tgaaacagtt caggacgtgc 3181 tgctcctgga cccccgctgg ctctgcacaa acgtcctggg gaagttgctg tctgtggaga 3241 ccccacgggc gctgcaccac taccggggcc gctacaccgt ggaggacatc cagcgcctgg 3301 tgcccgacag cgacgtggag gagctgctgc agatcctcga tgccatggac atctgcgccc 3361 gggacctgag cagcgggacc atggtggacg tcccagccct gatcaagaca gacaacctgc 3421 accgctcctg ggctgatgag gaggacgagg tgatggtgta tggtggcgtg cgcatcgtgc 3481 ccgtggaaca cctcaccccc ttcccatgtg gcatctttca caaggtccag gtgaacctgt 3541 gccggtggat ccaccagcaa agcacagagg gcgacgcgga catccgcctg tgggtgaatg 3601 gctgcaagct ggccaaccgt ggggccgagc tgctggtgct gctggtcaac cacggccagg 3661 gcattgaggt ccaggtccgt ggcctggaga cggagaagat caagtgctgc ctgctgctgg 3721 actcggtgtg cagcaccatt gagaacgtca tggccaccac gctgccaggg ctcctgaccg 3781 tgaagcatta cctgagcccc cagcagctgc gggagcacca tgagcccgtc atgatctacc 3841 agccacggga cttcttccgg gcacagactc tgaaggaaac ctcactgacc aacaccatgg 3901 gggggtacaa ggaaagcttc agcagcatca tgtgcttcgg gtgtcacgac gtctactcac 3961 aggccagcct cggcatggac atccatgcat cagacctgaa cctcctcact cggaggaaac 4021 tgagtcgccT GCTGGACCCG CCCGACCCCC TGGGGAAGGA CTGGTGCCTT CTCGCCATGA 4081 ACTTAGGCCT CCCTGACCTC GTGGCAAAGT ACAACACCAA TAACGGGGCT CCCAAGGATT 4141 TCCTCCCCAG CCCCCTCCAC GCCCTGCTGC GGGAATGGAC CACCTACCCT GAGAGCACAG 4201 TGGGCACCCT CATGTCCAAA CTGAGGGAGC TGGGTCGCCG GGATGCCGCA GACTTTTTGC 4261 TGAAGGCATC CTCTGTGTTC AAAATCAACC TGGATGGCAA TGGCCAGGAG GCCTATGCCT 4321 CGAGCTGCAA CAGCGGCACC TCTTACAATT CCATTAGCTC TGTTGTATCC CGGTGACTAG 4381 TAAGGGTGGG CGCGCCGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 4441 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 4501 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATATTACAT AAACCATTCC 4561 CATTTGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt