Construct: ORF TRCN0000489781
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020557.1_s317c1
- DNA Barcode:
- CGGGCGTAAACGGCTAGCTTTTAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TTBK2 (146057)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489781
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 146057 | TTBK2 | tau tubulin kinase 2 | NM_173500.4 | 97.1% | 97.1% | 433_537del |
| 2 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_024449849.1 | 97.1% | 97.1% | 433_537del |
| 3 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_024449850.1 | 97.1% | 97.1% | 433_537del |
| 4 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_005254173.5 | 91.5% | 91.5% | (many diffs) |
| 5 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_006720403.4 | 91.5% | 91.4% | (many diffs) |
| 6 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_024449851.1 | 91.5% | 91.4% | (many diffs) |
| 7 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_006720402.4 | 90% | 90.2% | 1_133del;201_202ins148;418_522del |
| 8 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_017021950.2 | 89.7% | 89.7% | 0_1ins279;154_258del |
| 9 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_005254171.5 | 89.2% | 89.6% | 1_166del;234_235ins148;451_555del |
| 10 | mouse | 140810 | Ttbk2 | tau tubulin kinase 2 | NM_001024856.2 | 86.2% | 87% | (many diffs) |
| 11 | mouse | 140810 | Ttbk2 | tau tubulin kinase 2 | NM_001024857.2 | 86.2% | 87% | (many diffs) |
| 12 | mouse | 140810 | Ttbk2 | tau tubulin kinase 2 | NM_080788.3 | 81.7% | 82.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3699
- ORF length:
- 3627
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagtggg ggaggagagc agctggatat cctgagtgtt ggaatcctag 121 tgaaagaaag atggaaagtg ttgagaaaga ttgggggtgg gggctttgga gaaatttacg 181 atgccttgga catgctcacc agggaaaatg ttgcactgaa ggtggaatca gctcaacaac 241 caaaacaagt tctgaaaatg gaagttgctg ttttgaaaaa gctgcaaggg aaagaccatg 301 tttgtagatt tattggctgt gggaggaatg atcgattcaa ctatgtggtc atgcagttgc 361 agggtcggaa tctggcagat cttcgccgta gccagtcccg aggcacattc accattagta 421 ccactctccg gctgggtaga cagattttgg agtctattga aagcattcat tctgtgggat 481 tcttgcatcg agacatcaaa ccgcctcgag ctgtggcagg ttttcgaggg acagttcgtt 541 atgcatcaat caacgcacat cggaacaggg aaatgggaag acatgatgac ctttggtcct 601 tattctacat gttggtggag tttgtggttg gtcagctgcc ctggagaaaa ataaaggaca 661 aggagcaagt aggctctatt aaggagagat atgaccacag gctcatgttg aaacatctcc 721 ctccagaatt cagcatcttt ctagaccata tctcttcttt ggattatttt acaaaaccag 781 actaccagct tcttacatcc gtgtttgaca atagcatcaa gacttttgga gtaattgaga 841 gtgacccttt tgactgggag aagactggaa atgatggctc cctaacaacc accactactt 901 ctaccacccc tcagttgcac actcgcttga cccctgctgc aattggaatt gccaatgcta 961 ctcccatccc tggagacttg cttcgagaaa atacagatga ggtatttcca gatgaacagc 1021 ttagcgatgg agaaaatggc atccctgttg gtgtgtcacc agataaattg cctggatctc 1081 tgggacaccc ccgtccccag gagaaggatg tttgggaaga gatggatgcc aacaaaaaca 1141 agataaagct tggaatttgt aaggctgcta ctgaagagga gaacagccat ggccaggcaa 1201 atggtcttct caatgctcca agccttgggt caccaattcg tgtccgctca gagattactc 1261 agccagacag agatattcca ctggtgcgaa agttacgttc cattcacagc tttgagctgg 1321 aaaaacgtct gaccctggag ccaaagccag acactgacaa gttccttgag acctgcctgg 1381 agaaaatgca gaaagatacc agtgcaggaa aagaatctat tctccctgct ctgctgcata 1441 agccttgcgt tcctgctgtg tcccgtactg accacatctg gcactatgat gaagaatatc 1501 ttccagatgc ctccaagcct gcttctgcca acacccctga gcaggcagat ggtggtggca 1561 gcaatggatt tatagctgtt aacctgagct cttgcaagca agaaattgat tccaaagaat 1621 gggtgattgt ggacaaggag caggaccttc aggattttag gacaaatgag gctgtaggac 1681 ataaaacaac tggaagtcct tctgatgagg agcctgaagt acttcaagtc ctggaggcat 1741 cacctcaaga tgaaaagctc cagttaggtc cttgggcaga aaatgatcat ttaaagaagg 1801 aaacctcagg tgtggtctta gcactttctg cagagggtcc tcctactgct gcttcagaac 1861 aatatacaga taggctggaa ctccagcctg gagctgctag tcagtttatt gcagcgacgc 1921 ccacaagtct aatggaggcg caggcagaag gaccccttac agcgattaca attcctagac 1981 cttctgtggc atctacacag tcaacttcag gaagctttca ctgtggtcag cagccagaga 2041 agaaagatct tcagcccatg gagcccactg tggaacttta ctctccaagg gaaaacttct 2101 ctggcttggt tgtgacagag ggtgaacctc ctagtggagg aagcagaaca gatttggggc 2161 ttcagataga tcacattggt catgacatgt tacccaacat tagagaaagt aacaaatctc 2221 aagacctggg accaaaagaa cttcctgatc ataatagact ggttgtgaga gaatttgaaa 2281 atctccctgg ggaaactgaa gagaaaagca tccttttaga gtcagataat gaagatgaga 2341 agttaagtag agggcagcat tgtattgaga tctcctctct cccaggagat ttggtaattg 2401 tggaaaagga tcactcagct actactgaac ctcttgatgt gacaaaaaca cagactttta 2461 gtgtggtgcc aaatcaagac aaaaataatg agataatgaa gcttctgaca gttggaactt 2521 cagaaatttc ttccagagac attgacccac atgttgaagg tcagataggc caagtggcag 2581 aaatgcaaaa aaataagata tctaaggatg atgacatcat gagtgaagac ttgccaggtc 2641 atcaaggaga cctctctact tttttgcacc aagagggcaa gagagagaaa atcaccccta 2701 gaaatggaga actatttcat tgtgtttcag agaatgaaca tggtgcccca acccggaagg 2761 atatggttag gtcatccttt gtaactagac acagccgaat ccctgtttta gcacaagaga 2821 tagactcaac tttggaatca tcctctccag tttctgcaaa agaaaagctc ctccaaaaga 2881 aagcctatca gccagaccta gtcaagcttc tggtggaaaa aagacaattc aagtccttcc 2941 ttggcgacct ctcaagtgcc tctgataaat tgctagagga gaaactagct actgttcctg 3001 ctcccttttg tgaggaggaa gtgctcactc ccttttcaag actgacagta gattctcacc 3061 tgagtaggtc agctgaagat agctttctgt cacccatcat ctcccagtct agaaagagca 3121 aaattccaag gccagtttca tgggtcaaca cagatcaggt caatagctca acttcgtctc 3181 agttctttcc tcggccacca ccaggaaagc cacccacgag gcctggagta gaagccaggc 3241 tacgcagata taaagtccta gggagtagta actccgactc agaccttttc tcccgcctgg 3301 cccaaattct tcaaaatGGA TCTCAGAAAC CCCGGAGCAC TACTCAGTGC AAGAGTCCAG 3361 GATCTCCTCA CAATCCAAAA ACACCACCCA AGAGTCCAGT TGTCCCTCGC AGGAGTCCCA 3421 GTGCCTCTCC TCGAAGCTCA TCCTTGCCTC GCACGTCTAG TTCCTCACCA TCTAGGGCTG 3481 GACGGCCCCA CCATGACCAG AGGAGTTCGT CCCCACATCT GGGGAGAAGC AAGTCACCTC 3541 CCAGCCACTC AGGATCTTCC TCCTCCAGGA GGTCCTGCCA ACAGGAGCAT TGCAAACCCA 3601 GCAAGAATGG CCTGAAAGGA TCCGGCAGCC TCCACCACCA CTCAGCCAGC ACTAAAACCC 3661 CCCAAGGGAA GAGTAAGCCA GCCAGTAAAC TCAGCAGATA GACTAGTGAC CCAGCTTTCT 3721 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 3781 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 3841 GTGGAAAGGA CGACGGGCGT AAACGGCTAG CTTTTACACG CGTTAAGTCg acaatcaacc 3901 tctggattac aaaatttgtg aaagatt