Construct: ORF TRCN0000489783
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019299.1_s317c1
- DNA Barcode:
- CCTAGTAAAGACAGGGTTTATGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRHR (7201)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489783
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7201 | TRHR | thyrotropin releasing hormo... | NM_003301.6 | 100% | 100% | |
| 2 | mouse | 22045 | Trhr | thyrotropin releasing hormo... | XM_006520747.1 | 90.2% | 94.4% | (many diffs) |
| 3 | mouse | 22045 | Trhr | thyrotropin releasing hormo... | NM_013696.2 | 89.2% | 93.2% | (many diffs) |
| 4 | mouse | 22045 | Trhr | thyrotropin releasing hormo... | XM_006520748.2 | 59.7% | 62% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1269
- ORF length:
- 1194
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggaa aacgagacag tcagtgaact gaaccaaaca cagcttcagc 121 cacgagcagt ggtggcctta gaataccagg tggtcaccat cttacttgta ctcattattt 181 gtggcctggg cattgtaggc aacatcatgg tagtcctggt tgtcatgaga accaagcaca 241 tgaggacccc cacaaactgc tacctggtga gcctggcagt agctgatctc atggtcttgg 301 tggccgcagg cctccccaac ataacagaca gtatctacgg ttcctgggtc tatggctatg 361 ttggatgcct ctgcattact tacctccagt atttgggaat taatgcatcc tcttgttcaa 421 taacagcctt taccattgag aggtacatag caatctgtca ccccatcaaa gcccagtttc 481 tctgcacatt ttccagagcc aaaaagatta tcatctttgt ctgggctttc acatctcttt 541 actgtatgct ctggttcttc ttgctggatc tcaatattag cacctacaaa gatgctattg 601 tgatatcctg tggctacaag atctccagga attactactc acctatttac ctaatggact 661 ttggtgtctt ttatgttgtg ccaatgatcc tggctaccgt cctctatgga ttcatagcta 721 gaatcctttt cttaaatccc attccttcag atcctaaaga aaactctaag acatggaaaa 781 atgattcaac ccatcagaac acaaatctga atgtaaatac ctctaataga tgtttcaaca 841 gcacagtatc ttcaaggaag caggtcacca agatgctggc agtggttGTA ATTCTGTTTG 901 CCCTTTTATG GATGCCCTAC AGGACTCTAG TGGTTGTCAA CTCATTTCTC TCCAGTCCTT 961 TCCAAGAAAA TTGGTTTTTG CTCTTTTGCA GAATTTGCAT TTATCTCAAC AGTGCCATCA 1021 ACCCGGTGAT TTACAATCTC ATGTCCCAGA AATTCCGTGC AGCCTTCAGA AAGCTCTGCA 1081 ACTGCAAGCA GAAGCCAACA GAGAAACCTG CTAACTACAG TGTGGCCCTA AATTACAGCG 1141 TCATCAAGGA GTCAGACCAT TTCAGCACAG AGCTTGATGA TATCACTGTC ACTGACACTT 1201 ACCTGTCTGC CACAAAAGTG TCTTTTGATG ACACCTGCTT GGCTTCTGAG GTATCCTTTA 1261 GCCAAAGTGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1321 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1381 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCTAGT AAAGACAGGG TTTATGTGAC 1441 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt