Construct: ORF TRCN0000489840
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021759.1_s317c1
- DNA Barcode:
- GCGCTGGCTCACACTAGACAAAAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NOTCH3 (4854)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489840
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4854 | NOTCH3 | notch receptor 3 | NM_000435.3 | 99.8% | 99.9% | (many diffs) |
| 2 | human | 4854 | NOTCH3 | notch receptor 3 | XM_005259924.4 | 97.6% | 97.6% | (many diffs) |
| 3 | mouse | 18131 | Notch3 | notch 3 | NM_008716.3 | 84.3% | 90.8% | (many diffs) |
| 4 | mouse | 18131 | Notch3 | notch 3 | XM_017317293.1 | 83.9% | 90.3% | (many diffs) |
| 5 | mouse | 18131 | Notch3 | notch 3 | XM_030249555.1 | 71.3% | 77% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 7032
- ORF length:
- 6963
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggggccgggg gcccgtggcc gccgccgccg ccgtcgcccg atgtcgccgc 121 caccgccacc gccacccgtg cgggcgctgc ccctgctgct gctactagcg gggccggggg 181 ctgcagcccc cccttgcctg gacggaagcc cgtgtgcaaa tggaggtcgt tgcacccagc 241 tgccctcccg ggaggctgcc tgcctgtgcc cgcctggctg ggtgggtgag cggtgtcagc 301 tggaggaccc ctgtcactca ggcccctgtg ctggccgtgg tgtctgccag agttcagtgg 361 tggctggcac tgcccgattc tcatgccggt gcccccgtgg cttccgaggc cctgactgct 421 ccctgccaga tccctgcctc agcagccctt gtgcccacgg tgcccgctgc tcagtggggc 481 ccgatggacg cttcctctgc tcctgcccac ctggctacca gggccgcagc tgccgaagcg 541 acgtggatga gtgccgggtg ggtgagccct gccgccatgg tggcacctgc ctcaacacac 601 ctggctcctt ccgctgccag tgtccagctg gctacacagg gccactatgt gagaaccccg 661 cggtgccctg tgcgccctca ccatgccgta acgggggcac ctgcaggcag agtggcgacc 721 tcacttacga ctgtgcctgt cttcctgggt ttgagggtca gaattgtgaa gtgaacgtgg 781 acgactgtcc aggacaccga tgtctcaatg gggggacatg cgtggatggc gtcaacacct 841 ataactgcca gtgccctcct gagtggacag gccagttctg cacggaggac gtggatgagt 901 gtcagctgca gcccaacgcc tgccacaatg ggggtacctg cttcaacacg ctgggtggcc 961 acagctgcgt gtgtgtcaat ggctggacag gcgagagctg cagtcagaat atcgatgact 1021 gtgccacagc cgtgtgcttc catggggcca cctgccatga ccgcgtggct tctttctact 1081 gtgcctgccc catgggcaag actggcctcc tgtgtcacct ggatgacgcc tgtgtcagca 1141 acccctgcca cgaggatgct atctgtgaca caaatccggt gaacggccgg gccatttgca 1201 cctgtcctcc cggcttcacg ggtggggcat gtgaccagga tgtggacgag tgctctatcg 1261 gcgccaaccc ctgcgagcac ttgggcaggt gcgtgaacac gcagggctcc ttcctgtgcc 1321 agtgcggtcg tggctacact ggacctcgct gtgagaccga tgtcaacgag tgtctgtcgg 1381 ggccctgccg aaaccaggcc acgtgcctcg accgcatagg ccagttcacc tgtatctgta 1441 tggcaggctt cacaggaacc tattgcgagg tggacattga cgagtgtcag agtagcccct 1501 gtgtcaacgg tggggtctgc aaggaccgag tcaatggctt cagctgcacc tgcccctcgg 1561 gcttcagcgg ctccacgtgt cagctggacg tggacgaatg cgccagcacg ccctgcagga 1621 atggcgccaa atgcgtggac cagcccgatg gctacgagtg ccgctgtgcc gagggctttg 1681 agggcacgct gtgtgatcgc aacgtggacg actgctcccc tgacccatgc caccatggtc 1741 gctgcgtgga tggcatcgcc agcttctcat gtgcctgtgc tcctggctac acgggcacac 1801 gctgcgagag ccaggtggac gaatgccgca gccagccctg ccgccatggc ggcaaatgcc 1861 tagacctggt ggacaagtac ctctgccgct gcccttctgg gaccacaggt gtgaactgcg 1921 aagtgaacat tgacgactgt gccagcaacc cctgcacctt tggagtctgc cgtgatggca 1981 tcaaccgcta cgactgtgtc tgccaacctg gcttcacagg gcccctttgt aacgtggaga 2041 tcaatgagtg tgcttccagc ccatgcggcg agggaggttc ctgtgtggat ggggaaaatg 2101 gcttccgctg cctctgcccg cctggctcct tgcccccact ctgcctcccc ccgagccatc 2161 cctgtgccca tgagccctgc agtcacggca tctgctatga tgcacctggc gggttccgct 2221 gtgtgtgtga gcctggctgg agtggccccc gctgcagcca gagcctggcc cgagacgcct 2281 gtgagtccca gccgtgcagg gccggtggga catgcagcag cgatggaatg ggtttccact 2341 gcacctgccc gcctggtgtc cagggacgtc agtgtgaact cctctccccc tgcaccccga 2401 acccctgtga gcatgggggc cgctgcgagt ctgcccctgg ccagctgcct gtctgctcct 2461 gcccccaggg ctggcaaggc ccacgatgcc agcaggatgt ggacgagtgt gctggccccg 2521 caccctgtgg ccctcatggt atctgcacca acctggcagg gagtttcagc tgcacctgcc 2581 atggagggta cactggccct tcctgtgatc aggacatcaa tgactgtgac cccaacccat 2641 gcctgaacgg tggctcgtgc caagacggcg tgggctcctt ttcctgctcc tgcctccctg 2701 gtttcgccgg cccacgatgc gcccgcgatg tggatgagtg cctgagcaac ccctgcggcc 2761 cgggcacctg taccgaccac gtggcctcct tcacctgcac ctgcccgccg ggctacggag 2821 gcttccactg cgaacaggac ctgcccgact gcagccccag ctcctgcttc aatggcggga 2881 cctgtgtgga cggcgtgaac tcgttcagct gcctgtgccg tcccggctac acaggagccc 2941 actgccaaca tgaggcagac ccctgcctct cgcggccctg cctacacggg ggcgtctgca 3001 gcgccgccca ccctggcttc cgctgcacct gcctcgagag cttcacgggc ccgcagtgcc 3061 agacgctggt ggattggtgc agccgccagc cttgtcaaaa cgggggtcgc tgcgtccaga 3121 ctggggccta ttgcctttgt ccccctggat ggagcggacg cctctgtgac atccgaagct 3181 tgccctgcag ggaggccgca gcccagatcg gggtgcggct ggagcagctg tgtcaggcgg 3241 gtgggcagtg tgtggatgaa gacagctccc actactgcgt gtgcccagag ggccgtactg 3301 gtagccactg tgagcaggag gtggacccct gcttggccca gccctgccag catgggggga 3361 cctgccgtgg ctatatgggg ggctacatgt gtgagtgtct tcctggctac aatggtgata 3421 actgtgagga cgacgtggac gagtgtgcct cccagccctg ccagcacggg ggttcatgca 3481 ttgacctcgt ggcccgctat ctctgctcct gtcccccagg aacgctgggg gtgctctgcg 3541 agattaatga ggatgactgc ggcccaggcc caccgctgga ctcagggccc cggtgcctac 3601 acaatggcac ctgcgtggac ctggtgggtg gtttccgctg cacctgtccc ccaggataca 3661 ctggtttgcg ctgcgaggca gacatcaatg agtgtcgctc aggtgcctgc cacgcggcac 3721 acacccggga ctgcctgcag gacccaggcg gaggtttccg ttgcctttgt catgctggct 3781 tctcaggtcc tcgctgtcag actgtcctgt ctccctgcga gtcccagcca tgccagcatg 3841 gaggccagtg ccgtcctagc ccgggtcctg ggggtgggct gaccttcacc tgtcactgtg 3901 cccagccgtt ctggggtccg cgttgcgagc gggtggcgcg ctcctgccgg gagctgcagt 3961 gcccggtggg cgtcccatgc cagcagacgc cccgcgggcc gcgctgcgcc tgccccccag 4021 ggttgtcggg accctcctgc cgcagcttcc cggggtcgcc gccgggggcc agcaacgcca 4081 gctgcgcggc cgccccctgt ctccacgggg gctcctgccg ccccgcgccg ctcgcgccct 4141 tcttccgctg cgcttgcgcg cagggctgga ccgggccgcg ctgcgaggcg cccgccgcgg 4201 cacccgaggt ctcggaggag ccgcggtgcc cgcgcgccgc ctgccaggcc aagcgcgggg 4261 accagcgctg cgaccgcgag tgcaacagcc caggctgcgg ctgggacggc ggcgactgct 4321 cgctgagcgt gggcgacccc tggcggcaat gcgaggcgct gcagtgctgg cgcctcttca 4381 acaacagccg ctgcgacccc gcctgcagct cgcccgcctg cctctacgac aacttcgact 4441 gccacgccgg tggccgcgag cgcacttgca acccggtgta cgagaagtac tgcgccgacc 4501 actttgccga cggccgctgc gaccagggct gcaacacgga ggagtgcggc tgggatgggc 4561 tggattgtgc cagcgaggtg ccggccctgc tggcccgcgg cgtgctggtg ctcacagtgc 4621 tgctgccgcc ggaggagcta ctgcgttcca gcgccgactt tctgcagcgg ctcagcgcca 4681 tcctgcgcac ctcgctgcgc ttccgcctgg acgcgcacgg ccaggccatg gtcttccctt 4741 accaccggcc tagtcctggc tccgaacccc gggcccgtcg ggagctggcc cccgaggtga 4801 tcggctcggt agtaatgctg gagattgaca accggctctg cctgcagtcg cctgagaatg 4861 atcactgctt ccccgatgcc cagagcgccg ctgactacct gggagcgttg tcagcggtgg 4921 agcgcctgga cttcccgtac ccactgcggg acgtgcgggg ggagccgctg gagcctccag 4981 aacccagcgt cccgctgctg ccactgctag tggcgggcgc tgtcttgctg ctggtcattc 5041 tcgtcctggg tgtcatggtg gcccggcgca agcgcgagca cagcaccctc tggttccctg 5101 agggcttctc actgcacaag gacgtggcct ctggtcacaa gggccggcgg gaacccgtgg 5161 gccaggacgc gctgggcatg aagaacatgg ccaagggtga gagcctgatg ggggaggtgg 5221 ccacagactg gatggacaca gagtgcccag aggccaagcg gctaaaggta gaggagccag 5281 gcatgggggc tgaggaggct gtggattgcc gtcagtggac tcaacaccat ctggttgctg 5341 ctgacatccg cgtggcacca gccatggcac tgacaccacc acagggcgac gcagatgctg 5401 atggcatgga tgtcaatgtg cgtggcccag atggcttcac cccgctaatg ctggcttcct 5461 tctgtggggg ggctctggag ccaatgccaa ctgaagagga tgaggcagat gacacatcag 5521 ctagcatcat ctccgacctg atctgccagg gggctcagct tggggcacgg actgaccgta 5581 ctggcgagac tgctttgcac ctggctgccc gttatgcccg tgctgatgca gccaagcggc 5641 tgctggatgc tggggcagac accaatgccc aggaccactc aggccgcact cccctgcaca 5701 cagctgtcac agccgatgcc cagggtgtct tccagattct catccgaaac cgctctacag 5761 acttggatgc ccgcatggca gatggctcaa cggcactgat cctggcggcc cgcctggcag 5821 tagagggcat ggtggaagag ctcatcgcca gccatgctga tgtcaatgct gtggatgagc 5881 ttgggaaatc agccttacac tgggctgcgg ctgtgaacaa cgtggaagcc actttggccc 5941 tgctcaaaaa tggagccaat aaggacatgc aggatagcaa ggaggagacc cccctattcc 6001 tggccgcccg cgagggcagc tatgaggctg ccaagctgct gttggaccac tttgccaacc 6061 gtgagatcac cgaccacctg gacaggctgc cgcgggacgt agcccaggag agactgcacc 6121 aggacatcgt gcgcttgctg gatcaaccca gtgggccccg cagccccccc ggtccccacg 6181 gcctggggcc tctgctctgt cctccagggg ccttcctccc tggcctcaaa gcggcacagt 6241 cggggtccaa gaagagcagg aggccccccg ggaaggcggg gctggggccg caggggcccc 6301 gggggcgggg caagaagctg acgctggcct gcccgggccc cctggctgac agctcggtca 6361 cgctgtcgcc cgtggactcg ctggactccc cgcggccttt cggtgggccc cctgcttccc 6421 ctggtggctt cccccttgag gggccctatg cagctgccac tgccactgca gtgtctctgg 6481 cacagcttgg tggcccaggc cgggcgggtc tagggcgcca gccccctgga ggatgtgtac 6541 tcagcctggg cctgctgaac cctgtggctg tgcccctcga ttgggcccgg ctgcccccac 6601 ctgcccctcc aggcccctcg ttcctgctgc cactggcgcc gggaccccag ctgctcaacc 6661 cagggacccc cgtctccccg caggagcggc ccccgcctta cctggcagtc ccaggacatg 6721 gcgaggagta cccggtggct ggggcacaca gcagcccccc aaaggcccgc ttccTGCGGG 6781 TTCCCAGTGA GCACCCTTAC CTGACCCCAT CCCCCGAATC CCCTGAGCAC TGGGCCAGCC 6841 CCTCACCTCC CTCCCTCTCA GACTGGTCCG AATCCACGCC TAGCCCAGCC ACTGCCACTG 6901 GGGCCATGGC CACCACCACT GGGGCACTGC CTGCCCAGCC ACTTCCCTTG TCTGTTCCCA 6961 GCTCCCTTGC TCAGGCCCAG ACCCAGCTGG GGCCCCAGCC GGAAGTTACC CCCAAGAGGC 7021 AAGTGTTGGC CTGAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 7081 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 7141 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GCGCTGGCTC ACACTAGACA 7201 AAAGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt