Construct: ORF TRCN0000489925
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020890.1_s317c1
- DNA Barcode:
- CATCATACTGCGAACTCACATAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PTGFR (5737)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489925
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5737 | PTGFR | prostaglandin F receptor | NM_000959.4 | 100% | 100% | |
| 2 | human | 5737 | PTGFR | prostaglandin F receptor | NM_001039585.2 | 81.5% | 75.7% | (many diffs) |
| 3 | human | 5737 | PTGFR | prostaglandin F receptor | XM_017001873.1 | 80.1% | 75.4% | (many diffs) |
| 4 | human | 5737 | PTGFR | prostaglandin F receptor | XR_001737338.1 | 57.1% | 1_335del;1134_1274del;1363_1364ins190 | |
| 5 | human | 5737 | PTGFR | prostaglandin F receptor | XR_946715.2 | 53.5% | (many diffs) | |
| 6 | mouse | 19220 | Ptgfr | prostaglandin F receptor | NM_008966.3 | 84% | 86.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1149
- ORF length:
- 1077
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtccatg aacaattcca aacagctagt gtctcctgca gctgcgcttc 121 tttcaaacac aacctgccag acggaaaacc ggctttccgt atttttttca gtaatcttca 181 tgacagtggg aatcttgtca aacagccttg ccatcgccat tctcatgaag gcatatcaga 241 gatttagaca gaagtccaag gcatcgtttc tgcttttggc cagtggcctg gtaatcactg 301 atttctttgg ccatctcatc aatggagcca tagcagtatt tgtatatgct tctgataaag 361 aatggatccg ctttgaccaa tcaaatgtcc tttgcagtat ttttggtatc tgcatggtgt 421 tttctggtct gtgcccactt cttctaggca gtgtgatggc cattgagcgg tgtattggag 481 tcacaaaacc aatatttcat tctacgaaaa ttacatccaa acatgtgaaa atgatgttaa 541 gtggtgtgtg cttgtttgct gttttcatag ctttgctgcc catccttgga catcgagact 601 ataaaattca ggcgtcgagg acctggtgtt tctacaacac agaagacatc aaagactggg 661 aagatagatt ttatcttcta cttttttctt ttctggggct cttagccctt gGTGTTTCAT 721 TGTTGTGCAA TGCAATCACA GGAATTACAC TTTTAAGAGT TAAATTTAAA AGTCAGCAGC 781 ACAGACAAGG CAGATCTCAT CATTTGGAAA TGGTAATCCA GCTCCTGGCG ATAATGTGTG 841 TCTCCTGTAT TTGTTGGAGC CCATTTCTGG TTACAATGGC CAACATTGGA ATAAATGGAA 901 ATCATTCTCT GGAAACCTGT GAAACAACAC TTTTTGCTCT CCGAATGGCA ACATGGAATC 961 AAATCTTAGA TCCTTGGGTA TATATTCTTC TACGAAAGGC TGTCCTTAAG AATCTCTATA 1021 AGCTTGCCAG TCAATGCTGT GGAGTGCATG TCATCAGCTT ACATATTTGG GAGCTTAGTT 1081 CCATTAAAAA TTCCTTAAAG GTTGCTGCTA TTTCTGAGTC ACCAGTTGCA GAGAAATCAG 1141 CAAGCACCTA GGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1201 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1261 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAT CATACTGCGA ACTCACATAA 1321 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t