Construct: ORF TRCN0000489949
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020019.1_s317c1
- DNA Barcode:
- GCATCGCACCCAACTGGGATACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSNK1A1 (1452)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489949
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1452 | CSNK1A1 | casein kinase 1 alpha 1 | NM_001892.6 | 99.9% | 99.7% | 1011_1012insG |
| 2 | human | 1452 | CSNK1A1 | casein kinase 1 alpha 1 | NM_001271741.2 | 95.7% | 95.8% | 972_974delTTT;975_976ins40 |
| 3 | human | 1452 | CSNK1A1 | casein kinase 1 alpha 1 | NM_001025105.3 | 92.2% | 92% | 455_538del;1095_1096insG |
| 4 | human | 122011 | CSNK1A1L | casein kinase 1 alpha 1 like | NM_145203.6 | 91.4% | 90.2% | (many diffs) |
| 5 | human | 1452 | CSNK1A1 | casein kinase 1 alpha 1 | NM_001271742.2 | 67.8% | 67.7% | 0_1ins267;188_271del;828_829insG |
| 6 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_006526388.2 | 94.8% | 99.7% | (many diffs) |
| 7 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | NM_146087.2 | 90.7% | 95.8% | (many diffs) |
| 8 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_006526384.2 | 87.5% | 92% | (many diffs) |
| 9 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_006526385.2 | 87.3% | 91.8% | (many diffs) |
| 10 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_006526383.3 | 85.5% | 89.8% | (many diffs) |
| 11 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_011247048.2 | 85.4% | 89.8% | (many diffs) |
| 12 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_006526386.3 | 84.9% | 88.3% | (many diffs) |
| 13 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_006526387.2 | 83.8% | 88.5% | (many diffs) |
| 14 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XR_386023.3 | 13.8% | (many diffs) | |
| 15 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XR_386022.3 | 13.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1083
- ORF length:
- 1014
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcgagtagc agcggctcca aggctgaatt cattgtcgga gggaaatata 121 aactggtacg gaagatcggg tctggctcct tcggggacat ctatttggcg atcaacatca 181 ccaacggcga ggaagtggca gtgaagctag aatctcagaa ggccaggcat ccccagttgc 241 tgtacgagag caagctctat aagattcttc aaggtggggt tggcatcccc cacatacggt 301 ggtatggtca ggaaaaagac tacaatgtac tagtcatgga tcttctggga cctagcctcg 361 aagacctctt caatttctgt tcaagaaggt tcacaatgaa aactgtactt atgttagctg 421 accagatgat cagtagaatt gaatatgtgc atacaaagaa ttttatacac agagacatta 481 aaccagataa cttcctaatg ggtattgggc gtcactgtaa taagttattc cttattgatt 541 ttggtttggc caaaaagtac agagacaaca ggacaaggca acacatacca tacagagaag 601 ataaaaacct cactggcact gcccgatatg ctagcatcaa tgcacatctt ggtattgagc 661 agagtcgccg agatgacatG GAATCATTAG GATATGTTTT GATGTATTTT AATAGAACCA 721 GCCTGCCATG GCAAGGGCTA AAGGCTGCAA CAAAGAAACA AAAATATGAA AAGATTAGTG 781 AAAAGAAGAT GTCCACGCCT GTTGAAGTTT TATGTAAGGG GTTTCCTGCA GAATTTGCGA 841 TGTACTTAAA CTATTGTCGT GGGCTACGCT TTGAGGAAGC CCCAGATTAC ATGTATCTGA 901 GGCAGCTATT CCGCATTCTT TTCAGGACCC TGAACCATCA ATATGACTAC ACATTTGATT 961 GGACAATGTT AAAGCAGAAA GCAGCACAGC AGGCAGCCTC TTCCAGTGGG CAGGGTCAGC 1021 AGGCCCAAAC CCCCACAGGC AAGCAAACTG ACAAAACCAA GAGTAACATG AAAGGTTTCG 1081 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1141 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1201 TTTATATATC TTGTGGAAAG GACGAGCATC GCACCCAACT GGGATACTCA CGCGTTAAGT 1261 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt