Construct: ORF TRCN0000491252
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021686.2_s317c1
- DNA Barcode:
- ACTCGGAAAATATGCCAGGCTATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TEK (7010)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491252
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7010 | TEK | TEK receptor tyrosine kinase | NM_001290078.1 | 31% | .2% | 1_2020del |
| 2 | human | 7010 | TEK | TEK receptor tyrosine kinase | XM_005251563.2 | 28% | .1% | 1_2332del |
| 3 | human | 7010 | TEK | TEK receptor tyrosine kinase | NM_001290077.1 | 27.9% | .1% | 1_2335del |
| 4 | human | 7010 | TEK | TEK receptor tyrosine kinase | XM_005251561.2 | 26.9% | .1% | 1_2461del |
| 5 | human | 7010 | TEK | TEK receptor tyrosine kinase | NM_000459.4 | 26.9% | .1% | 1_2464del |
| 6 | mouse | 21687 | Tek | endothelial-specific recept... | XM_006502930.2 | 30.4% | .3% | (many diffs) |
| 7 | mouse | 21687 | Tek | endothelial-specific recept... | NM_001290551.1 | 25.4% | .1% | (many diffs) |
| 8 | mouse | 21687 | Tek | endothelial-specific recept... | NM_001290549.1 | 24.3% | .2% | (many diffs) |
| 9 | mouse | 21687 | Tek | endothelial-specific recept... | NM_013690.3 | 24.3% | .2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 178
- ORF end:
- 199
- ORF length:
- 21
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcca aaacgtgagg gaagaaccag ctgtgcagtt caactcaggg actctggccc 121 taaacaggaa ggtcaaaaac aacccagatc ctacaattta tccagtgctt gactggaatg 181 acatcaaatt tcaagatgtg attggggagg gcaattttgg ccaagttctt aaggcgcgca 241 tcaagaagga tgggttacgg atggatgctg ccatcaaaag aatgaaagaa tatgcctcca 301 aagatgatca cagggacttt gcaggagaac tggaagttct ttgtaaactt ggacaccatc 361 caaacatcat caatctctta ggagcatgtg aacatcgagg ctacttgtac ctggccattg 421 agtacgcgcc ccatggaaac cttctggact tccttcgcaa gagccgtgtg ctggagacgg 481 acccagcatt tgccattgcc aatagcaccg cgtccacact gtcctcccag cagctccttc 541 acttcgctgc cgacgtggcc cggggcatgg actacttgag ccaaaaacag tttatccaca 601 gggatctggc tgccagaaac attttagttg gtgaaaacta tgtggcaaaa atagcagatt 661 ttggattgtc ccgaggtcaa gaggtgtatg tgaaaaagac aatgggaagg ctcccagtgc 721 gctggatggc catcgagtca ctgaattaca gtgtgtacac aaccaacagt gatgtatggt 781 cctatggtgt gttactatgg gagattgtta gcttaggagg cacaccctac tgcgggatga 841 cttgtgcaga actcTACGAG AAGCTGCCCC AGGGCTACAG ACTGGAGAAG CCCCTGAACT 901 GTGATGATGA GGTGTATGAT CTAATGAGAC AATGCTGGCG GGAGAAGCCT TATGAGAGGC 961 CATCATTTGC CCAGATATTG GTGTCCTTAA ACAGAATGTT AGAGGAGCGA AAGACCTACG 1021 TGAATACCAC GCTTTATGAG AAGTTTACTT ATGCAGGAAT TGACTGTTCT GCTGAAGAAG 1081 CGGCCTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1141 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1201 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACTCGG AAAATATGCC AGGCTATCAC 1261 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt