Transcript: Human NM_000459.4

Homo sapiens TEK receptor tyrosine kinase (TEK), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-10
Taxon:
Homo sapiens (human)
Gene:
TEK (7010)
Length:
4787
CDS:
451..3825

Additional Resources:

NCBI RefSeq record:
NM_000459.4
NBCI Gene record:
TEK (7010)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146784 TACTCGGCCAGGTATATAGG pXPR_003 AGG 581 17% 4 0.6794 TEK TEK 75722
2 BRDN0001148630 TGGCACAGGAACACCCATAG pXPR_003 GGG 854 25% 6 0.4802 TEK TEK 75723
3 BRDN0001148973 AGACCACTCTAAATTTGACC pXPR_003 TGG 1683 50% 12 0.207 TEK TEK 75724
4 BRDN0001149392 GCCTGAAACAGCATACCAGG pXPR_003 TGG 2125 63% 13 0.0723 TEK TEK 75725
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_000459.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000356282 GTGTACTCGGCCAGGTATATA pLKO_005 1012 CDS 100% 15.000 21.000 N TEK n/a
2 TRCN0000000413 GCTACCTACTAATGAAGAAAT pLKO.1 1578 CDS 100% 13.200 18.480 N TEK n/a
3 TRCN0000356218 TACGTGAATACCACGCTTTAT pLKO_005 3754 CDS 100% 13.200 18.480 N TEK n/a
4 TRCN0000000414 CGCTACCTACTAATGAAGAAA pLKO.1 1577 CDS 100% 5.625 7.875 N TEK n/a
5 TRCN0000000415 GCTTCTATACAAACCCGTTAA pLKO.1 1890 CDS 100% 10.800 8.640 N TEK n/a
6 TRCN0000356278 CGTGAGTACAATTAGTATAAT pLKO_005 4112 3UTR 100% 15.000 10.500 N TEK n/a
7 TRCN0000378503 CCGGCATGAAGTACCTGATAT pLKO_005 948 CDS 100% 13.200 9.240 N Tek n/a
8 TRCN0000195300 CCTCCTCCAAGAGGTCTAAAT pLKO.1 2080 CDS 100% 13.200 9.240 N TEK n/a
9 TRCN0000000416 CCTACCAGCTACTTTAACTAT pLKO.1 822 CDS 100% 5.625 3.938 N TEK n/a
10 TRCN0000023554 CGCATCAAGAAGGATGGGTTA pLKO.1 2974 CDS 100% 4.050 2.835 N Tek n/a
11 TRCN0000000412 TGGGTGACATTTGGGAGACAT pLKO.1 4594 3UTR 100% 4.950 2.970 N TEK n/a
12 TRCN0000023557 GCCTTAATGAACCAGCACCAA pLKO.1 637 CDS 100% 2.640 2.112 N Tek n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_000459.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491252 ACTCGGAAAATATGCCAGGCTATC pLX_317 23.4% 26.9% .1% V5 (not translated due to prior stop codon) 1_2464del n/a
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