Construct: ORF TRCN0000491255

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020695.2_s317c1
DNA Barcode:
TGAGGTATCTGACCGTTGGGTATT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
ADCK5 (203054)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491255
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 203054 ADCK5 aarF domain containing kina... NM_174922.5 99.9% 99.8% 51C>A
2 human 203054 ADCK5 aarF domain containing kina... XM_011516907.3 88.5% 88.3% (many diffs)
3 human 203054 ADCK5 aarF domain containing kina... XM_011516909.1 88.4% 88.4% 0_1ins201
4 human 203054 ADCK5 aarF domain containing kina... XM_017013174.1 88.4% 88.4% 0_1ins201
5 human 203054 ADCK5 aarF domain containing kina... XR_002956603.1 82.8% (many diffs)
6 human 203054 ADCK5 aarF domain containing kina... XM_006716527.2 81.5% 80.5% 0_1ins316;24_25insAGAAC
7 human 203054 ADCK5 aarF domain containing kina... XM_011516910.1 81.5% 80.5% 0_1ins316;24_25insAGAAC
8 human 203054 ADCK5 aarF domain containing kina... XR_928305.3 64.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1812
ORF length:
1740
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgtggcga ccggtgcagc tctgtcattt ccactctgct ctgctgcaca
121gaaggcagaa gccctggccg tcccctgctg tgttcttcag gagaaacgtc aggggccttc
181ctccaaggtt ctccagcccc acacccctgt ggaggaaggt gctctccacc gcggtagtgg
241gggcgcccct gctcctcgga gcccgctatg tcatggcaga ggcacgggag aagaggagga
301tgcggctcgt ggtggatggc atggggcgct ttggcaggtc tctgaaggtc ggcctgcaga
361tctccctgga ctactggtgg tgcaccaatg ttgtccttcg aggggtggaa gagaacagcc
421caggctactt ggaggtgatg tctgcgtgtc accagcgggc ggctgatgcc ctggtggcag
481gggccatcag caacgggggc ctctacgtga agctgggcca ggggctgtgc tccttcaacc
541acctgcttcc ccccgagtat acccggaccc tgcgcgtgct agaggacagg gccctcaagc
601ggggcttcca ggaggtggat gagttgttcc ttgaggactt ccaggccctc ccccacgagc
661tcttccagga gtttgactac cagccaattg ctgccgccag cctggcacag gtgcacagag
721ccaagctgca cgatggcacc agcgtggctg tgaaggtgca gtacatcgac ctgcgggacc
781gctttgatgg ggacatccac accctggagc tcctgctgcg gctcgttgag gtcatgcacc
841ccagctttgg cttcagctgg gtcctccagg acctgaaggg gaccctggcc caggagctgg
901acttcgagaa tgagggccgc aacgcagagc gctgtgcgcg ggagctggcg cacttcccct
961acgtcgtggt gccccgcgtg cactgggaca agtccagcaa gcgcgtgctc actgccgact
1021tctgcgccgg ctgcaaggtc aacgatgtgg aggccatcag gagccagggg ctggcagtgc
1081atgacatagc agaaaagctc atcaaggcct ttgctgagca gatattttac accggcttca
1141tccactcgga cccacatcct ggcaacgttc tggtgcggaa aggcccggac gggaaagcgg
1201agctggtgct gctggaccac gggctctacc agttcctgga ggagaaggac cgcgcagccc
1261tctgccagct gtggcgggcc atcatcctgc gggacgacgc cgccatgagg gcgcacgcag
1321ccgcactggg ggtgcaagac tacctcctgt tcgccgagat gctcatgcag cgccccgtgc
1381gcctggggca gctgtggggc tcgcacctac tgagccgcga agaggcggcc tacatggtgg
1441acatggcccg cgagcgcTTC GAGGCCGTCA TGGCGGTGCT CAGGGAGCTG CCGCGGCCCA
1501TGCTGCTGGT GCTGCGCAAC ATCAACACCG TGCGCGCTAT CAACGTGGCC CTCGGCGCCC
1561CCGTGGACCG CTACTTCCTT ATGGCTAAAA GGGCTGTCCG GGGCTGGAGC CGCCTGGCGG
1621GCGCCACGTA TCGGGGTGTC TACGGCACCA GCCTCCTGCG CCACGCCAAG GTCGTCTGGG
1681AGATGCTCAA GTTTGAAGTG GCGCTCAGGC TGGAGACCTT GGCCATGCGG CTGACCGCCC
1741TCCTGGCTCG TGCTCTGGTC CACCTGAGCC TCGTGCCCCC AGCGGAGGAG CTCTACCAGT
1801ACCTGGAGAC CTAGAACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
1861CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
1921TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGAGGTATCT GACCGTTGGG
1981TATTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.