Construct: ORF TRCN0000491260
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021652.2_s317c1
- DNA Barcode:
- AAACCCATAGATCGAGCTTCCTTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CSNK2A1 (1457)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491260
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1457 | CSNK2A1 | casein kinase 2 alpha 1 | NM_001362770.2 | 100% | 100% | |
| 2 | human | 1457 | CSNK2A1 | casein kinase 2 alpha 1 | NM_001362771.2 | 100% | 100% | |
| 3 | human | 1457 | CSNK2A1 | casein kinase 2 alpha 1 | NM_001895.4 | 100% | 100% | |
| 4 | human | 1457 | CSNK2A1 | casein kinase 2 alpha 1 | NM_177559.3 | 100% | 100% | |
| 5 | human | 283106 | CSNK2A3 | casein kinase 2 alpha 3 | NM_001256686.2 | 99.3% | 98.4% | (many diffs) |
| 6 | human | 1457 | CSNK2A1 | casein kinase 2 alpha 1 | NM_177560.3 | 65.2% | 65.2% | 0_1ins408 |
| 7 | mouse | 12995 | Csnk2a1 | casein kinase 2, alpha 1 po... | NM_007788.3 | 94.5% | 98.7% | (many diffs) |
| 8 | mouse | 12995 | Csnk2a1 | casein kinase 2, alpha 1 po... | XM_006498657.4 | 94.5% | 98.7% | (many diffs) |
| 9 | mouse | 12995 | Csnk2a1 | casein kinase 2, alpha 1 po... | XM_011239272.3 | 94.5% | 98.7% | (many diffs) |
| 10 | mouse | 12995 | Csnk2a1 | casein kinase 2, alpha 1 po... | XM_030247074.1 | 94.5% | 98.7% | (many diffs) |
| 11 | mouse | 12995 | Csnk2a1 | casein kinase 2, alpha 1 po... | XM_030247075.1 | 94.5% | 98.7% | (many diffs) |
| 12 | mouse | 12995 | Csnk2a1 | casein kinase 2, alpha 1 po... | XM_030247076.1 | 94.5% | 98.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1242
- ORF length:
- 1173
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gtcgggaccc gtgccaagca gggccagagt ttacacagat gttaatacac 121 acagacctcg agaatactgg gattacgagt cacatgtggt ggaatgggga aatcaagatg 181 actaccagct ggttcgaaaa ttaggccgag gtaaatacag tgaagtattt gaagccatca 241 acatcacaaa taatgaaaaa gttgttgtta aaattctcaa gccagtaaaa aagaagaaaa 301 ttaagcgtga aataaagatt ttggagaatt tgagaggagg tcccaacatc atcacactgg 361 cagacattgt aaaagaccct gtgtcacgaa cccccgcctt ggtttttgaa cacgtaaaca 421 acacagactt caagcaattg taccagacgt taacagacta tgatattcga ttttacatgt 481 atgagattct gaaggccctg gattattgtc acagcatggg aattatgcac agagatgtca 541 agccccataa tgtcatgatt gatcatgagc acagaaagct acgactaata gactggggtt 601 tggctgagtt ttatcatcct ggccaagaat ataatgtccg agttgcttcc cgatacttca 661 aaggtcctga gctacttgta gactatcaga tgtacgatta tagtttggat atgtggagtt 721 tgggttgtat gctggcaagt atgatctttc ggaaggagcc atttttccat ggacatgaca 781 attatgatca gttggtgagg atagccaagg ttctggggac agaagattta tatgactata 841 ttgacaaata caacattgaa ttagatccac gtttcaatga tatcttgggc agacactctc 901 gaaagcgatg ggaacgcttt gtccacagtg aaaatcagca ccttgtcagc ccTGAGGCCT 961 TGGATTTCCT GGACAAACTG CTGCGATATG ACCACCAGTC ACGGCTTACT GCAAGAGAGG 1021 CAATGGAGCA CCCCTATTTC TACACTGTTG TGAAGGACCA GGCTCGAATG GGTTCATCTA 1081 GCATGCCAGG GGGCAGTACG CCCGTCAGCA GCGCCAATAT GATGTCAGGG ATTTCTTCAG 1141 TGCCAACCCC TTCACCCCTT GGACCTCTGG CAGGCTCACC AGTGATTGCT GCTGCCAACC 1201 CCCTTGGGAT GCCTGTTCCA GCTGCCGCTG GCGCTCAGCA GTAGAACCCA GCTTTCTTGT 1261 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1321 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1381 GAAAGGACGA AAACCCATAG ATCGAGCTTC CTTGACGCGT TAAGTCgaca atcaacctct 1441 ggattacaaa atttgtgaaa gatt