Construct: ORF TRCN0000491266
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019099.2_s317c1
- DNA Barcode:
- TCCCTCTTGAAAGCGGACAAACCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- STRADA (92335)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491266
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001003787.4 | 70.2% | 82.9% | 1096_1097ins293;1140_1141ins253;1293_1294insG |
| 2 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001363786.1 | 68.9% | 81.1% | (many diffs) |
| 3 | human | 92335 | STRADA | STE20 related adaptor alpha | XM_011525466.3 | 65.7% | 92.1% | 1096_1097ins589;1209_1210ins42 |
| 4 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001363787.1 | 65.5% | 76.4% | (many diffs) |
| 5 | human | 92335 | STRADA | STE20 related adaptor alpha | XM_011525467.3 | 64.2% | 73.7% | (many diffs) |
| 6 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001003786.3 | 64.2% | 73.7% | (many diffs) |
| 7 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001003788.3 | 60.8% | 69.8% | (many diffs) |
| 8 | human | 92335 | STRADA | STE20 related adaptor alpha | XM_005257799.3 | 60.8% | 69.8% | (many diffs) |
| 9 | human | 92335 | STRADA | STE20 related adaptor alpha | NR_156741.2 | 59.2% | (many diffs) | |
| 10 | human | 92335 | STRADA | STE20 related adaptor alpha | XR_243688.3 | 57.8% | (many diffs) | |
| 11 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_153335.6 | 56.7% | 89.3% | 12_13ins111;1044_1045ins685 |
| 12 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001363788.1 | 55.6% | 87.7% | (many diffs) |
| 13 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001165969.2 | 50.9% | 80.2% | (many diffs) |
| 14 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001363789.1 | 49.6% | 77.1% | (many diffs) |
| 15 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001165970.2 | 48.4% | 76.3% | (many diffs) |
| 16 | human | 92335 | STRADA | STE20 related adaptor alpha | XM_005257801.5 | 47.4% | 74.5% | (many diffs) |
| 17 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001363790.1 | 47% | 71.2% | (many diffs) |
| 18 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001363791.1 | 46.1% | 72.7% | (many diffs) |
| 19 | human | 92335 | STRADA | STE20 related adaptor alpha | XM_017025314.2 | 42.6% | 67% | (many diffs) |
| 20 | human | 92335 | STRADA | STE20 related adaptor alpha | XM_005257803.5 | 37.8% | 51% | (many diffs) |
| 21 | mouse | 72149 | Strada | STE20-related kinase adapto... | NM_001252448.1 | 62.4% | 81.1% | (many diffs) |
| 22 | mouse | 72149 | Strada | STE20-related kinase adapto... | XM_011249270.1 | 62.4% | 81.1% | (many diffs) |
| 23 | mouse | 72149 | Strada | STE20-related kinase adapto... | XM_006534293.3 | 61.2% | 79.3% | (many diffs) |
| 24 | mouse | 72149 | Strada | STE20-related kinase adapto... | XM_006534294.3 | 57.9% | 74.4% | (many diffs) |
| 25 | mouse | 72149 | Strada | STE20-related kinase adapto... | NM_028126.3 | 56.5% | 71.7% | (many diffs) |
| 26 | mouse | 72149 | Strada | STE20-related kinase adapto... | XM_006534295.1 | 56.5% | 71.7% | (many diffs) |
| 27 | mouse | 72149 | Strada | STE20-related kinase adapto... | NM_001252449.1 | 54.7% | 72.5% | (many diffs) |
| 28 | mouse | 72149 | Strada | STE20-related kinase adapto... | XM_006534296.3 | 53.7% | 68.7% | (many diffs) |
| 29 | mouse | 72149 | Strada | STE20-related kinase adapto... | XM_006534297.3 | 53.7% | 68.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1227
- ORF length:
- 1155
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcattt cttgtaagta aaccagagcg aatcaggcgg tgggtctcgg 121 aaaagttcat tgttgagggc ttaagagatt tggaactatt tggagagcag cctccgggtg 181 acactcggag aaaaaccaat gatgcgagct cagagtcaat agcatccttc tctaaacagg 241 aggtcatgag tagctttctg ccagagggag ggtgttacga gctgctcact gtgataggca 301 aaggatttga ggacctgatg actgtgaatc tagcaaggta caaaccaaca ggagagtacg 361 tgactgtacg gaggattaac ctagaagctt gttccaatga gatggtaaca ttcttgcagg 421 gcgagctgca tgtctccaaa ctcttcaacc atcccaatat cgtgccatat cgagccactt 481 ttattgcaga caatgagctg tgggttgtca catcattcat ggcatacggt tctgcaaaag 541 atctcatctg tacacacttc atggatggca tgaatgagct ggcgattgct tacatcctgc 601 agggggtgct gaaggccctc gactacatcc accacatggg atatgtacac aggagtgtca 661 aagccagcca catcctgatc tctgtggatg ggaaggtcta cctgtctggt ttgcgcagca 721 acctcagcat gataagccat gggcagcggc agcgagtggt ccacgatttt cccaagtaca 781 gtgtcaaggt tctgccgtgg ctcagccccg aggtcctcca gcagaatctc cagggttatg 841 atgccaagtc tgacatctac agtgtgggaa tcacagcctg tgaactggcc aacggccatg 901 tcccctttaa ggatatgcct gccacccaga tgctgctaga gaaactgaac ggcacagtgc 961 cctgcctgtt ggataccagc accatccccg ctgaggagct gaccatgagc ccttcgcgct 1021 cagtggccaa ctctggcctg agtgacagcc tgaccaccag caccccccgg ccctccaacg 1081 gtgactcgcc ctcccacccc taccaccgaa ccttctcccc ccacttccac cactttgtgg 1141 agcagtgcct tcagcgcaac ccggatgcca ggtatccctg ctggcctggg cctgggcttc 1201 gggagagcag agggtgctca ggagggtaag gccagggtgt gaagggactt acctcccaaa 1261 ggttctgcag gggaatctgg agctacacac aggagggatc agctcctggg tgtgtcagag 1321 gccagcctgg ggagctctgg ccactgcttc ccatgagctg agggagaggg agaggggacc 1381 cgaggctgag gcataagtgg caggatttcg ggaagctggg gacacggcag tgatgctgcg 1441 gtctctcctc ccctttccct ccaggcccag tgccagcacc ctcctgaacc actctttctt 1501 caagcaggta tcgtagcccc ttcgttctgg ttctggttct agttctggtt ctaacaactc 1561 acaatccctt tagctttctc tcccctccct ttgaatgaga gaaactaccc cgcttccgaa 1621 gcccctgaaa gacactgctc cttcctctca tggagttggc TCCGACAGCC CGTCTGCCAC 1681 CAGGCCATGG TTCCTTGCCC CATGGTGTCC TGGGACCCAG AGCAACAGGA TCTGTCACCC 1741 ACCTCTCTCT TCTCCCCCAG ATCAAGCGAC GTGCCTCAGA GGCTTTGCCC GAATTGCTTC 1801 GTCCTGTCAC CCCCATCACC AATTTTGAGG GCAGCCAGTC TCAGGACCAC AGTGGAATCT 1861 TTGGCCTGGT AACAAACCTG GAAGAGCTGG AGGTGGACGA TTGGGAGTTC GACCCAGCTT 1921 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1981 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2041 CTTGTGGAAA GGACGATCCC TCTTGAAAGC GGACAAACCA ACGCGTTAAG TCgacaatca 2101 acctctggat tacaaaattt gtgaaagatt