Construct: ORF TRCN0000491286
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020652.2_s317c1
- DNA Barcode:
- CGCCGTCACCATTGCTGTGGGGGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAP3K9 (4293)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491286
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4293 | MAP3K9 | mitogen-activated protein k... | NM_001284230.1 | 95.9% | 99.9% | 1752_1754delGGA;1833G>A;3312_3313ins134 |
| 2 | human | 4293 | MAP3K9 | mitogen-activated protein k... | NM_033141.4 | 94.8% | 98.6% | (many diffs) |
| 3 | human | 4293 | MAP3K9 | mitogen-activated protein k... | XM_005267683.5 | 93.9% | 97.8% | (many diffs) |
| 4 | human | 4293 | MAP3K9 | mitogen-activated protein k... | XM_011536788.3 | 91% | 94.5% | (many diffs) |
| 5 | human | 4293 | MAP3K9 | mitogen-activated protein k... | NM_001284231.1 | 67.1% | 69.2% | (many diffs) |
| 6 | human | 4293 | MAP3K9 | mitogen-activated protein k... | XM_011536792.1 | 65.8% | 68.3% | (many diffs) |
| 7 | human | 4293 | MAP3K9 | mitogen-activated protein k... | NM_001284232.1 | 63.9% | 66.3% | (many diffs) |
| 8 | human | 4293 | MAP3K9 | mitogen-activated protein k... | XM_011536794.2 | 61.1% | 63.5% | (many diffs) |
| 9 | mouse | 338372 | Map3k9 | mitogen-activated protein k... | XM_006516036.3 | 86% | 94.6% | (many diffs) |
| 10 | mouse | 338372 | Map3k9 | mitogen-activated protein k... | NM_001174107.1 | 84.2% | 92.5% | (many diffs) |
| 11 | mouse | 338372 | Map3k9 | mitogen-activated protein k... | XM_017315122.1 | 84.2% | 91% | (many diffs) |
| 12 | mouse | 338372 | Map3k9 | mitogen-activated protein k... | XM_006516037.3 | 65.3% | 67.9% | (many diffs) |
| 13 | mouse | 338372 | Map3k9 | mitogen-activated protein k... | NM_177395.5 | 48.2% | 53.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 96
- ORF end:
- 3405
- ORF length:
- 3309
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcacctcta gagccatgga gccctccaga gcgcttctcg 121 gctgcctagc gagcgccgcc gctgccgccc cgccggggga ggatggagca ggggccgggg 181 ccgaggagga ggaggaggag gaggaggagg cggcggcggc ggtgggcccc ggggagctgg 241 gctgcgacgc gccgctgccc tactggacgg ccgtgttcga gtacgaggcg gcgggcgagg 301 acgagctgac cctgcggctg ggcgacgtgg tggaggtgct gtccaaggac tcgcaggtgt 361 ccggcgacga gggctggtgg accgggcagc tgaaccagcg ggtgggcatc ttccccagca 421 actacgtgac cccgcgcagc gccttctcca gccgctgcca gcccggcggc gaggacccca 481 gttgctaccc gcccattcag ttgttagaaa ttgattttgc ggagctcacc ttggaagaga 541 ttattggcat cgggggcttt gggaaggtct atcgtgcttt ctggataggg gatgaggttg 601 ctgtgaaagc agctcgccac gaccctgatg aggacatcag ccagaccata gagaatgttc 661 gccaagaggc caagctcttc gccatgctga agcaccccaa catcattgcc ctaagagggg 721 tatgtctgaa ggagcccaac ctctgcttgg tcatggagtt tgctcgtgga ggacctttga 781 atagagtgtt atctgggaaa aggattcccc cagacatcct ggtgaattgg gctgtgcaga 841 ttgccagagg gatgaactac ttacatgatg aggcaattgt tcccatcatc caccgcgacc 901 ttaagtccag caacatattg atcctccaga aggtggagaa tggagacctg agcaacaaga 961 ttctgaagat cactgatttt ggcctggctc gggaatggca ccgaaccacc aagatgagtg 1021 cggcagggac gtatgcttgg atggcacccg aagtcatccg ggcctccatg ttttccaaag 1081 gcagtgatgt gtggagctat ggggtgctac tttgggagtt gctgactggt gaggtgccct 1141 ttcgaggcat tgatggctta gcagtcgctt atggagtggc catgaacaaa ctcgcccttc 1201 ctattccttc tacgtgccca gaaccttttg ccaaactcat ggaagactgc tggaatcctg 1261 atccccactc acgaccatct ttcacgaata tcctggacca gctaaccacc atagaggagt 1321 ctggtttctt tgaaatgccc aaggactcct tccactgcct gcaggacaac tggaaacacg 1381 agattcagga gatgtttgac caactcaggg ccaaagaaaa ggaacttcgc acctgggagg 1441 aggagctgac gcgggctgca ctgcagcaga agaaccagga ggaactgctg cggcgtcggg 1501 agcaggagct ggccgagcgg gagattgaca tcctggaacg ggagctcaac atcatcatcc 1561 accagctgtg ccaggagaag ccccgggtga agaaacgcaa gggcaagttc aggaagagcc 1621 ggctgaagct caaggatggc aaccgcatca gcctcccttc tgatttccag cacaagttca 1681 cggtgcaggc ctcccctacc atggataaaa ggaagagtct tatcaacagc cgctccagtc 1741 ctcctgcaag ccccaccatc attcctcgcc ttcgagccat ccagttgaca ccaggtgaaa 1801 gcagcaaaac ctggggcagg agctcagtcg tcccaaagga ggaaggggag gaggagaaga 1861 gggccccaaa gaagaaggga cggacgtggg ggccagggac gcttggtcag aaggagcttg 1921 cctcaggaga tgaaggatcc cctcagagac gtgagaaagc taatggttta agtaccccat 1981 cagaatctcc acatttccac ttgggcctca agtccctggt agatggatat aagcagtggt 2041 cgtccagtgc ccccaacctg gtgaagggcc caaggagtag cccggccctg ccagggttca 2101 ccagccttat ggagatggag gatgaggaca gtgaaggccc agggagtgga gagagtcgcc 2161 tacagcattc acccagccag tcctacctct gtatcccatt ccctcgtgga gaggatggcg 2221 atggcccctc cagtgatgga atccatgagg agcccacccc agtcaactcg gccacgagta 2281 cccctcagct gacgccaacc aacagcctca agcggggcgg tgcccaccac cgccgctgcg 2341 aggtggctct gctcggctgt ggggctgttc tggcagccac aggcctaggg tttgacttgc 2401 tggaagctgg caagtgccag ctgcttcccc tggaggagcc tgagccacca gcccgggagg 2461 agaagaaaag acgggagggt ctttttcaga ggtccagccg tcctcgtcgg agcaccagcc 2521 ccccatcccg aaagcttttc aagaaggagg agcccatgct gttgctagga gacccctctg 2581 cctccctgac gctgctctcc ctctcctcca tctccgagtg caactccaca cgctccctgc 2641 tgcgctccga cagcgatgaa attgtcgtgt atgagatgcc agtcagccca gtcgaggccc 2701 ctcccctgag tccatgtacc cacaaccccc tggtcaatgt ccgagtagag cgcttcaaac 2761 gagatcctaa ccaatctctg actcccaccc atgtcaccct caccaccccc tcgcagccca 2821 gcagtcaccg gcggactcct tctgatgggg cccttaagcc agagactctc ctagccagca 2881 ggagcccctc cagcaatggg ttgagcccca gtcctggagc aggaatgttg aaaaccccca 2941 gtcccagccg agacccaggt gaattccccc gtctccctga ccccaatgtg gtcttccccc 3001 caaccccaag gcgctggaac actcagcagg actctacctt ggagagaccc aagactctgg 3061 agtttctgcc tcggccgcgt ccttctgcca accggcaacg gctggaccct tggtggtttg 3121 tgtcccccag ccatgcccgc agcacctccc cagccaacag cTCCAGCACA GAGACGCCCA 3181 GCAACCTGGA CTCCTGCTTT GCTAGCAGTA GCAGCACTGT AGAGGAGCGG CCTGGACTTC 3241 CAGCCCTGCT CCCGTTCCAG GCAGGGCCGC TGCCCCCGAC TGAGCGGACG CTCCTGGACC 3301 TGGATGCAGA GGGGCAGAGT CAGGACAGCA CCGTGCCGCT GTGCAGAGCG GAACTGAACA 3361 CACACAGGCC TGCCCCTTAT GAGATCCAGC AGGAGTTCTG GTCTTAGCAC GAAAAGGATT 3421 GGGGCGGGCA AGGGGGACAG CCAGCAGAGA TGAGGGGAGC TGGCGGGCAC AGCCCTTTCT 3481 CAGGGTTGGA CCCCCTGAGA TCCAGCCCTA CTTCTTGCAC TGATAATGCA CTTTGATCTA 3541 GAAAGGGTGG GCGCGCCGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 3601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 3661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACGCCGTC ACCATTGCTG 3721 TGGGGGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt