Construct: ORF TRCN0000491287

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019848.2_s317c1
DNA Barcode:
TTTAATTGATCTAATCTACTCAAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAPT (4137)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491287
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4137 MAPT microtubule associated prot... NM_001123067.3 99.7% 99.5% 597G>T;827G>A;1236_1237insG
2 human 4137 MAPT microtubule associated prot... NM_005910.5 93.2% 92.9% (many diffs)
3 human 4137 MAPT microtubule associated prot... NM_016834.5 92.7% 92.4% (many diffs)
4 human 4137 MAPT microtubule associated prot... NM_001203251.2 92.3% 92.2% 597G>T;735_736ins93;1143_1144insG
5 human 4137 MAPT microtubule associated prot... NM_001203252.1 86.2% 86.1% (many diffs)
6 human 4137 MAPT microtubule associated prot... XM_005257370.4 85.9% 85.8% (many diffs)
7 human 4137 MAPT microtubule associated prot... NM_016841.5 85.2% 85.2% (many diffs)
8 human 4137 MAPT microtubule associated prot... XM_005257369.4 81% 80.9% (many diffs)
9 human 4137 MAPT microtubule associated prot... XM_005257371.4 79.9% 79.7% (many diffs)
10 human 4137 MAPT microtubule associated prot... NM_016835.4 54.2% 54.1% (many diffs)
11 human 4137 MAPT microtubule associated prot... NM_001123066.3 52.9% 52.8% (many diffs)
12 human 4137 MAPT microtubule associated prot... XM_005257367.4 51.6% 51.5% (many diffs)
13 human 4137 MAPT microtubule associated prot... XM_005257366.3 50.5% 45.8% (many diffs)
14 human 4137 MAPT microtubule associated prot... XM_005257364.4 49.3% 49.2% (many diffs)
15 human 4137 MAPT microtubule associated prot... XM_005257368.4 47.8% 47.8% (many diffs)
16 human 4137 MAPT microtubule associated prot... XM_005257362.4 47.7% 47.6% (many diffs)
17 human 4137 MAPT microtubule associated prot... XM_005257365.4 44.1% 44.1% (many diffs)
18 mouse 17762 Mapt microtubule-associated prot... XM_006532407.3 83.1% 88.1% (many diffs)
19 mouse 17762 Mapt microtubule-associated prot... NM_001038609.2 77.6% 82.4% (many diffs)
20 mouse 17762 Mapt microtubule-associated prot... NM_010838.4 77.4% 82.4% (many diffs)
21 mouse 17762 Mapt microtubule-associated prot... XM_006532409.2 75.9% 80.9% (many diffs)
22 mouse 17762 Mapt microtubule-associated prot... NM_001285455.1 73% 77.8% (many diffs)
23 mouse 17762 Mapt microtubule-associated prot... XM_006532408.3 70.9% 75.6% (many diffs)
24 mouse 17762 Mapt microtubule-associated prot... NM_001285456.1 70.3% 75.1% (many diffs)
25 mouse 17762 Mapt microtubule-associated prot... NM_001285454.1 66.7% 71.1% (many diffs)
26 mouse 17762 Mapt microtubule-associated prot... XM_006532410.3 44.7% 47.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1308
ORF length:
1239
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat ggctgagccc cgccaggagt tcgaagtgat ggaagatcac gctgggacgt
121acgggttggg ggacaggaaa gatcaggggg gctacaccat gcaccaagac caagagggtg
181acacggacgc tggcctgaaa gaatctcccc tgcagacccc cactgaggac ggatctgagg
241aaccgggctc tgaaacctct gatgctaaga gcactccaac agcggaagct gaagaagcag
301gcattggaga cacccccagc ctggaagacg aagctgctgg tcacgtgacc caagctcgca
361tggtcagtaa aagcaaagac gggactggaa gcgatgacaa aaaagccaag ggggctgatg
421gtaaaacgaa gatcgccaca ccgcggggag cagcccctcc aggccagaag ggccaggcca
481acgccaccag gattccagca aaaaccccgc ccgctccaaa gacaccaccc agctctggtg
541aacctccaaa atcaggggat cgcagcggct acagcagccc cggctcccca ggcactcccg
601gcagccgctc ccgcaccccg tcccttccaa ccccacccac ccgggagccc aagaaggtgg
661cagttgtccg tactccaccc aagtcgccgt cttccgccaa gagccgcctg cagacagccc
721ccgtgcccat gccagacctg aagaatgtca agtccaagat cggctccact gagaacctga
781agcaccagcc gggaggcggg aaggtgcaga taattaataa gaagctggat cttagcaacg
841tccagtccaa gtgtggctca aaggataata tcaaacacgt cccgggaggc ggcaatgtgc
901aaatagtcta caaaccagtt gacctgagca aggtgacctc caagtgtggc tcattaggca
961acatccatca taaaccagga ggtggccagg tggaagtaaa atcTGAGAAG CTTGACTTCA
1021AGGACAGAGT CCAGTCGAAG ATTGGGTCCC TGGACAATAT CACCCACGTC CCTGGCGGAG
1081GAAATAAAAA GATTGAAACC CACAAGCTGA CCTTCCGCGA GAACGCCAAA GCCAAGACAG
1141ACCACGGGGC GGAGATCGTG TACAAGTCGC CAGTGGTGTC TGGGGACACG TCTCCACGGC
1201ATCTCAGCAA TGTCTCCTCC ACCGGCAGCA TCGACATGGT AGACTCGCCC CAGCTCGCCA
1261CGCTAGCTGA CGAGGTGTCT GCCTCCCTGG CCAAGCAGGG TTTGGACCCA GCTTTCTTGT
1321ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1381AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1441GAAAGGACGA TTTAATTGAT CTAATCTACT CAATACGCGT TAAGTCgaca atcaacctct
1501ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)