Construct: ORF TRCN0000491291
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004510.1_s317c1
- Derived from:
- ccsbBroadEn_11993
- DNA Barcode:
- TGCCCGGATGGCTATCTTCTTGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTF2 (51507)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491291
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51507 | RTF2 | replication termination fac... | NM_001283037.1 | 15.4% | 12.1% | (many diffs) |
| 2 | human | 51507 | RTF2 | replication termination fac... | NM_001283036.1 | 11.7% | 9.1% | (many diffs) |
| 3 | human | 51507 | RTF2 | replication termination fac... | NM_016407.4 | 11.6% | 9.1% | (many diffs) |
| 4 | human | 51507 | RTF2 | replication termination fac... | NM_001283035.1 | 10.2% | 8.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 192
- ORF length:
- 126
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaAAAAG 61 TTGGCATGGG TTGCGACGGG GGAACAATCC CCAAGAGGCA TGAACTGGTG AAGGGGCCGA 121 AGAAGGTTGA GAAGGCAATT ATTGGTGGGC TCCTGGATGG GGCCTGGTCA CCAGAAACAC 181 CAAGCCAAGA TTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 241 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 301 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATGC CCGGATGGCT ATCTTCTTGA 361 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t