Construct: ORF TRCN0000491308

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020602.2_s317c1
DNA Barcode:
AAAACCCTGTGTGATATTATTCCA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
CDK8 (1024)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491308
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1024 CDK8 cyclin dependent kinase 8 NM_001260.3 100% 100%
2 human 1024 CDK8 cyclin dependent kinase 8 NM_001318368.2 99.7% 99.7% 1110_1111insAAG
3 human 1024 CDK8 cyclin dependent kinase 8 XM_017020317.2 72.8% 72.8% 0_1ins378
4 human 1024 CDK8 cyclin dependent kinase 8 NM_001346501.2 62.7% 62.7% 0_1ins519
5 human 1024 CDK8 cyclin dependent kinase 8 XM_011534865.2 62.7% 62.7% 0_1ins519
6 human 1024 CDK8 cyclin dependent kinase 8 XM_017020318.1 62.5% 62.5% 0_1ins519;591_592insAAG
7 mouse 264064 Cdk8 cyclin-dependent kinase 8 NM_153599.4 93.9% 98.9% (many diffs)
8 mouse 264064 Cdk8 cyclin-dependent kinase 8 NM_001359990.1 93.7% 98.7% (many diffs)
9 mouse 264064 Cdk8 cyclin-dependent kinase 8 XM_006504836.4 92.8% 97.8% (many diffs)
10 mouse 264064 Cdk8 cyclin-dependent kinase 8 XM_006504838.4 91.3% 96.1% (many diffs)
11 mouse 264064 Cdk8 cyclin-dependent kinase 8 XM_006504839.4 86.7% 91.1% (many diffs)
12 mouse 264064 Cdk8 cyclin-dependent kinase 8 XM_006504840.4 83.6% 88.5% (many diffs)
13 mouse 264064 Cdk8 cyclin-dependent kinase 8 XM_030254515.1 83.4% 88.3% (many diffs)
14 mouse 264064 Cdk8 cyclin-dependent kinase 8 NM_001359991.1 80.6% 84.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1464
ORF length:
1392
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggactat gactttaaag tgaagctgag cagcgagcgg gagcgggtcg
121aggacctgtt tgaatacgag ggctgcaaag ttggccgagg cacttatggt cacgtctaca
181aagccaagag gaaagatggg aaggatgata aagactatgc tttaaaacaa atagaaggaa
241ctgggatctc tatgtcggca tgtagagaaa tagcattact tcgagagctt aagcatccaa
301acgtcatttc tcttcaaaag gtgtttctgt ctcatgctga taggaaggtg tggcttctgt
361ttgactatgc tgaacatgac ctctggcata taatcaagtt tcacagagct tctaaagcaa
421acaagaagcc agttcagtta cctcggggaa tggtgaagtc actattatat cagatcctag
481atggtattca ctacctgcat gctaactggg tgttgcacag agatttgaaa cctgctaata
541ttttagttat gggtgaaggt cctgagcgag gaagagtaaa aattgctgac atgggctttg
601cccgattatt taattcacct ttgaagcctt tagcagattt ggatccagtg gttgttacat
661tctggtaccg agcccctgaa ctacttcttg gagcaaggca ttataccaaa gctattgata
721tttgggctat agggtgtata tttgcagaac tactaacgtc agaaccaata tttcactgtc
781gacaagagga catcaaaact agtaatcctt atcaccatga ccagctggac agaatattca
841atgtaatggg atttcctgca gataaagatt gggaagatat aaaaaagatg cctgaacatt
901caacattaat gaaagatttc agaagaaata cgtataccaa ctgcagcctt atcaagtata
961tggaaaaaca taaagttaaa ccagatagta aagcattcca cttgcttcag aagctgctta
1021ccatggaccc aataaagcga attacctcag aacaggctat gcaggacccc tatttcttag
1081aagacccact tcctacatca gacgtttttg ccggttgtca aaTCCCTTAC CCAAAACGAG
1141AATTTTTAAC GGAAGAAGAA CCTGATGACA AAGGAGACAA AAAGAACCAG CAGCAGCAGC
1201AGGGCAATAA CCACACTAAT GGAACTGGCC ACCCAGGGAA TCAAGACAGC AGTCACACAC
1261AGGGACCCCC GTTGAAGAAA GTGAGAGTTG TTCCTCCTAC CACTACCTCA GGTGGACTTA
1321TCATGACCTC AGACTATCAG CGTTCCAATC CACATGCTGC CTATCCCAAC CCTGGACCAA
1381GCACATCACA GCCGCAGAGC AGCATGGGAT ACTCAGCTAC CTCCCAGCAG CCTCCACAGT
1441ACTCACATCA GACACATCGG TACTGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
1501GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1561AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAAAACCCT
1621GTGTGATATT ATTCCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1681aagatt

Download FASTA (ORF) (Full)

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