Construct: ORF TRCN0000491308
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020602.2_s317c1
- DNA Barcode:
- AAAACCCTGTGTGATATTATTCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CDK8 (1024)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491308
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1024 | CDK8 | cyclin dependent kinase 8 | NM_001260.3 | 100% | 100% | |
| 2 | human | 1024 | CDK8 | cyclin dependent kinase 8 | NM_001318368.2 | 99.7% | 99.7% | 1110_1111insAAG |
| 3 | human | 1024 | CDK8 | cyclin dependent kinase 8 | XM_017020317.2 | 72.8% | 72.8% | 0_1ins378 |
| 4 | human | 1024 | CDK8 | cyclin dependent kinase 8 | NM_001346501.2 | 62.7% | 62.7% | 0_1ins519 |
| 5 | human | 1024 | CDK8 | cyclin dependent kinase 8 | XM_011534865.2 | 62.7% | 62.7% | 0_1ins519 |
| 6 | human | 1024 | CDK8 | cyclin dependent kinase 8 | XM_017020318.1 | 62.5% | 62.5% | 0_1ins519;591_592insAAG |
| 7 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | NM_153599.4 | 93.9% | 98.9% | (many diffs) |
| 8 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | NM_001359990.1 | 93.7% | 98.7% | (many diffs) |
| 9 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_006504836.4 | 92.8% | 97.8% | (many diffs) |
| 10 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_006504838.4 | 91.3% | 96.1% | (many diffs) |
| 11 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_006504839.4 | 86.7% | 91.1% | (many diffs) |
| 12 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_006504840.4 | 83.6% | 88.5% | (many diffs) |
| 13 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_030254515.1 | 83.4% | 88.3% | (many diffs) |
| 14 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | NM_001359991.1 | 80.6% | 84.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1464
- ORF length:
- 1392
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggactat gactttaaag tgaagctgag cagcgagcgg gagcgggtcg 121 aggacctgtt tgaatacgag ggctgcaaag ttggccgagg cacttatggt cacgtctaca 181 aagccaagag gaaagatggg aaggatgata aagactatgc tttaaaacaa atagaaggaa 241 ctgggatctc tatgtcggca tgtagagaaa tagcattact tcgagagctt aagcatccaa 301 acgtcatttc tcttcaaaag gtgtttctgt ctcatgctga taggaaggtg tggcttctgt 361 ttgactatgc tgaacatgac ctctggcata taatcaagtt tcacagagct tctaaagcaa 421 acaagaagcc agttcagtta cctcggggaa tggtgaagtc actattatat cagatcctag 481 atggtattca ctacctgcat gctaactggg tgttgcacag agatttgaaa cctgctaata 541 ttttagttat gggtgaaggt cctgagcgag gaagagtaaa aattgctgac atgggctttg 601 cccgattatt taattcacct ttgaagcctt tagcagattt ggatccagtg gttgttacat 661 tctggtaccg agcccctgaa ctacttcttg gagcaaggca ttataccaaa gctattgata 721 tttgggctat agggtgtata tttgcagaac tactaacgtc agaaccaata tttcactgtc 781 gacaagagga catcaaaact agtaatcctt atcaccatga ccagctggac agaatattca 841 atgtaatggg atttcctgca gataaagatt gggaagatat aaaaaagatg cctgaacatt 901 caacattaat gaaagatttc agaagaaata cgtataccaa ctgcagcctt atcaagtata 961 tggaaaaaca taaagttaaa ccagatagta aagcattcca cttgcttcag aagctgctta 1021 ccatggaccc aataaagcga attacctcag aacaggctat gcaggacccc tatttcttag 1081 aagacccact tcctacatca gacgtttttg ccggttgtca aaTCCCTTAC CCAAAACGAG 1141 AATTTTTAAC GGAAGAAGAA CCTGATGACA AAGGAGACAA AAAGAACCAG CAGCAGCAGC 1201 AGGGCAATAA CCACACTAAT GGAACTGGCC ACCCAGGGAA TCAAGACAGC AGTCACACAC 1261 AGGGACCCCC GTTGAAGAAA GTGAGAGTTG TTCCTCCTAC CACTACCTCA GGTGGACTTA 1321 TCATGACCTC AGACTATCAG CGTTCCAATC CACATGCTGC CTATCCCAAC CCTGGACCAA 1381 GCACATCACA GCCGCAGAGC AGCATGGGAT ACTCAGCTAC CTCCCAGCAG CCTCCACAGT 1441 ACTCACATCA GACACATCGG TACTGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1501 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1561 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAAAACCCT 1621 GTGTGATATT ATTCCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1681 aagatt