Construct: ORF TRCN0000491319
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021619.2_s317c1
- DNA Barcode:
- GCTCCGACTGCGGGTACAATAATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GH1 (2688)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491319
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2688 | GH1 | growth hormone 1 | NM_000515.5 | 100% | 100% | |
| 2 | human | 2689 | GH2 | growth hormone 2 | NM_002059.5 | 94.6% | 93% | (many diffs) |
| 3 | human | 2688 | GH1 | growth hormone 1 | NM_022559.4 | 93% | 93% | 171_172ins45 |
| 4 | human | 1442 | CSH1 | chorionic somatomammotropin... | NM_001317.6 | 92.7% | 85.2% | (many diffs) |
| 5 | human | 1443 | CSH2 | chorionic somatomammotropin... | NM_020991.3 | 92.4% | 85.2% | (many diffs) |
| 6 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_022579.3 | 88.8% | 79.2% | (many diffs) |
| 7 | human | 2689 | GH2 | growth hormone 2 | NM_022556.4 | 88.1% | 86.6% | (many diffs) |
| 8 | human | 2689 | GH2 | growth hormone 2 | NM_022558.4 | 82.9% | 56.3% | (many diffs) |
| 9 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_022581.3 | 82.9% | 72.8% | (many diffs) |
| 10 | human | 2688 | GH1 | growth hormone 1 | NM_022560.4 | 81.5% | 81.5% | 171_172ins120 |
| 11 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_001321069.2 | 77.8% | 70.5% | (many diffs) |
| 12 | human | 2689 | GH2 | growth hormone 2 | NM_022557.4 | 69.3% | 49.8% | (many diffs) |
| 13 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_001321067.2 | 68.2% | 60.3% | (many diffs) |
| 14 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_001321068.2 | 68% | 61.7% | (many diffs) |
| 15 | human | 1443 | CSH2 | chorionic somatomammotropin... | NM_022644.3 | 64.4% | 58.2% | (many diffs) |
| 16 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_022580.3 | 58.5% | 52% | (many diffs) |
| 17 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_001318.4 | 54% | 49.3% | (many diffs) |
| 18 | human | 1443 | CSH2 | chorionic somatomammotropin... | NM_022645.2 | 52.3% | 49.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 720
- ORF length:
- 651
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggctacaggc tcccggacgt ccctgctcct ggcttttggc ctgctctgcc 121 tgccctggct tcaagagggc agtgccttcc caaccattcc cttatccagg ctttttgaca 181 acgctatgct ccgcgcccat cgtctgcacc agctggcctt tgacacctac caggagtttg 241 aagaagccta tatcccaaag gaacagaagt attcattcct gcagaacccc cagacctccc 301 tctgtttctc agagtctatt ccgacaccct ccaacaggga ggaaacacaa cagaaatcca 361 acctagagct gctccgcatc tccctgctgc tcatccagtc gtggctggag cccgtgcagt 421 tcctcaggag tgtcttcgcc aacagcctgg tgtacggcgc ctctgacagc aacgtctatg 481 acctccTAAA GGACCTAGAG GAAGGCATCC AAACGCTGAT GGGGAGGCTG GAAGATGGCA 541 GCCCCCGGAC TGGGCAGATC TTCAAGCAGA CCTACAGCAA GTTCGACACA AACTCACACA 601 ACGATGACGC ACTACTCAAG AACTACGGGC TGCTCTACTG CTTCAGGAAG GACATGGACA 661 AGGTCGAGAC ATTCCTGCGC ATCGTGCAGT GCCGCTCTGT GGAGGGCAGC TGTGGCTTCT 721 AGAACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 781 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 841 GGCTTTATAT ATCTTGTGGA AAGGACGAGC TCCGACTGCG GGTACAATAA TCACGCGTTA 901 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt