Construct: ORF TRCN0000491323
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020708.2_s317c1
- DNA Barcode:
- AAGTAGCCCTTGGGAAGAAAGCCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DSTYK (25778)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491323
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25778 | DSTYK | dual serine/threonine and t... | NM_199462.3 | 99.9% | 99.8% | 1921T>C |
| 2 | human | 25778 | DSTYK | dual serine/threonine and t... | NM_015375.3 | 95.1% | 95% | 1921T>C;2467_2601del |
| 3 | human | 25778 | DSTYK | dual serine/threonine and t... | XM_011509392.2 | 94.1% | 94% | 625_626ins27;1894T>C;2440_2574del |
| 4 | human | 25778 | DSTYK | dual serine/threonine and t... | XM_011509393.2 | 72.8% | 71.9% | (many diffs) |
| 5 | human | 25778 | DSTYK | dual serine/threonine and t... | XM_011509394.2 | 72.1% | 71.5% | (many diffs) |
| 6 | mouse | 213452 | Dstyk | dual serine/threonine and t... | NM_172516.4 | 84.1% | 86.6% | (many diffs) |
| 7 | mouse | 213452 | Dstyk | dual serine/threonine and t... | XM_006529356.3 | 74.7% | 77.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2724
- ORF length:
- 2652
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagggc gacggggtgc catggggcag cgagcccgtc tcgggtcccg 121 gccccggcgg cggcggaatg atccgcgagc tgtgccgggg cttcggccgc taccgccgct 181 acctgggacg gctgcgacag aacctgcgcg agacccagaa gttcttccgc gacatcaagt 241 gctcccacaa ccacacttgt ctctcctccc tcacgggcgg cggcggggcc gagcgcggcc 301 ctgcaggcga tgtcgccgaa accgggctgc aggcgggcca actgagctgc atttccttcc 361 cacctaagga agagaagtac ctccagcaga ttgtggactg cctcccttgc atactgatcc 421 tcggccagga ttgtaacgtc aagtgccagc tgttgaatct gctgttgggg gtgcaggtgc 481 ttcccaccac caagctgggc agtgaggaga gctgtaagct tcggcgcctc cgcttcacct 541 atgggactca gactcgggtc agcctggcgc tccctggaca gtatgaacta gtgcacacgc 601 tggttgctca tcagggcaac tgggagacca tccctgagga ggatctggag gtccaagaga 661 acaatgagga tgctgctcat gttttagcgg aactggaggt aacgatgcac catgctctct 721 tacaggaagt ggacgttgtg gtagcaccat gccaaggcct ccggcccaca gtggatgttc 781 tgggtgactt ggtgaatgat ttcttgcctg tgataaccta tgcactccac aaagatgaac 841 tctctgagag ggatgagcaa gagcttcagg aaatccgaaa gtatttctcc tttcctgtat 901 tctttttcaa agtgccgaaa ctgggctcgg agataataga ctcctcaacc aggagaatgg 961 agagcgaaag atcaccgctt tatcgccagc taattgacct gggctatctg agcagcagtc 1021 actggaactg tggggctcct ggccaggata ctaaagctca gagcatgttg gtggaacaga 1081 gtgaaaagct gagacacttg agcacatttt ctcaccaggt gttacagact cgcctggtgg 1141 atgcagccaa ggccctgaac ctggtgcact gccactgcct tgacatcttt attaaccagg 1201 catttgacat gcagcgggac ctgcagatca ctcccaaacg tctggaatat actcgaaaaa 1261 aggagaatga gttgtatgaa tcattgatga atattgccaa ccgaaagcag gaggaaatga 1321 aggatatgat tgttgagaca cttaatacca tgaaggagga acttctggat gatgctacta 1381 acatggagtt taaagacgtc attgtccctg agaatggaga accagtaggc accagagaga 1441 tcaaatgctg catccgacag atccaggaac tcatcatctc ccgacttaat caggcagtgg 1501 ctaataagct gatcagctca gtggattacc tgagggaaag cttcgtcgga accctggaac 1561 gatgtctgca gagcctggag aagtctcagg atgtctcagt tcacatcacc agtaattatc 1621 tcaaacagat cttaaatgct gcctatcatg ttgaagtcac gtttcactca gggtcgtcag 1681 ttacaaggat gctatgggag caaatcaaac agatcatcca gcgcatcaca tgggtgagcc 1741 cacctgccat cactctggaa tggaagagga aggtggccca ggaagccatt gagagcctca 1801 gcgcctccaa attggctaag agcatttgca gccaattccg gactcggctc aatagttccc 1861 acgaggcttt tgcagcctcc ttgcggcagc tggaagctgg ccactcaggc cggttagaga 1921 aaacggaaga tctatggctg agggttcgga aagatcatgc tccccgcctg gcccgccttt 1981 ctctggaaag ccgttcttta caggatgtct tgcttcatcg taaacctaaa ctgggacagg 2041 aactgggccg gggccagtat ggtgtggtat acctgtgtga caactgggga ggacacttcc 2101 cttgtgccct caaatcagtt gtccctccag atgagaagca ctggaatgat ctggctttgg 2161 aatttcacta tatgaggtct ctgccgaagc atgagcgatt ggtggatctc catggttcag 2221 tcattgacta caactatggt ggtggctcca gcattgctgt gctcctcatt atggagcggc 2281 tacaccggga tctctacaca gggctgaagg ctgggctgac cctGGAGACA CGTTTGCAGA 2341 TAGCACTAGA TGTGGTGGAG GGAATCCGCT TCCTGCACAG CCAGGGACTT GTCCATCGTG 2401 ATATCAAACT GAAAAATGTG CTGCTGGATA AGCAGAACCG TGCCAAGATC ACTGACTTAG 2461 GATTCTGCAA GCCAGAGGCC ATGATGTCAG GCAGCATTGT GGGGACACCA ATCCATATGG 2521 CCCCTGAACT TTTCACAGGG GCTCGCCCAG AACGTCTTCC TGTGTTTGAT GAGGAGTGCT 2581 GGCAGTTGAT GGAAGCCTGT TGGGATGGCG ACCCCTTGAA GAGGCCTCTC TTGGGCATTG 2641 TCCAGCCCAT GCTCCAGGGC ATCATGAATC GGCTCTGCAA GTCCAATTCT GAGCAGCCAA 2701 ACAGAGGACT AGATGATTCT ACTTAGAACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 2761 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2821 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAAGTAGCC 2881 CTTGGGAAGA AAGCCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2941 aagatt