Construct: ORF TRCN0000491323

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020708.2_s317c1
DNA Barcode:
AAGTAGCCCTTGGGAAGAAAGCCG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
DSTYK (25778)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491323
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 25778 DSTYK dual serine/threonine and t... NM_199462.3 99.9% 99.8% 1921T>C
2 human 25778 DSTYK dual serine/threonine and t... NM_015375.3 95.1% 95% 1921T>C;2467_2601del
3 human 25778 DSTYK dual serine/threonine and t... XM_011509392.2 94.1% 94% 625_626ins27;1894T>C;2440_2574del
4 human 25778 DSTYK dual serine/threonine and t... XM_011509393.2 72.8% 71.9% (many diffs)
5 human 25778 DSTYK dual serine/threonine and t... XM_011509394.2 72.1% 71.5% (many diffs)
6 mouse 213452 Dstyk dual serine/threonine and t... NM_172516.4 84.1% 86.6% (many diffs)
7 mouse 213452 Dstyk dual serine/threonine and t... XM_006529356.3 74.7% 77.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2724
ORF length:
2652
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggagggc gacggggtgc catggggcag cgagcccgtc tcgggtcccg
121gccccggcgg cggcggaatg atccgcgagc tgtgccgggg cttcggccgc taccgccgct
181acctgggacg gctgcgacag aacctgcgcg agacccagaa gttcttccgc gacatcaagt
241gctcccacaa ccacacttgt ctctcctccc tcacgggcgg cggcggggcc gagcgcggcc
301ctgcaggcga tgtcgccgaa accgggctgc aggcgggcca actgagctgc atttccttcc
361cacctaagga agagaagtac ctccagcaga ttgtggactg cctcccttgc atactgatcc
421tcggccagga ttgtaacgtc aagtgccagc tgttgaatct gctgttgggg gtgcaggtgc
481ttcccaccac caagctgggc agtgaggaga gctgtaagct tcggcgcctc cgcttcacct
541atgggactca gactcgggtc agcctggcgc tccctggaca gtatgaacta gtgcacacgc
601tggttgctca tcagggcaac tgggagacca tccctgagga ggatctggag gtccaagaga
661acaatgagga tgctgctcat gttttagcgg aactggaggt aacgatgcac catgctctct
721tacaggaagt ggacgttgtg gtagcaccat gccaaggcct ccggcccaca gtggatgttc
781tgggtgactt ggtgaatgat ttcttgcctg tgataaccta tgcactccac aaagatgaac
841tctctgagag ggatgagcaa gagcttcagg aaatccgaaa gtatttctcc tttcctgtat
901tctttttcaa agtgccgaaa ctgggctcgg agataataga ctcctcaacc aggagaatgg
961agagcgaaag atcaccgctt tatcgccagc taattgacct gggctatctg agcagcagtc
1021actggaactg tggggctcct ggccaggata ctaaagctca gagcatgttg gtggaacaga
1081gtgaaaagct gagacacttg agcacatttt ctcaccaggt gttacagact cgcctggtgg
1141atgcagccaa ggccctgaac ctggtgcact gccactgcct tgacatcttt attaaccagg
1201catttgacat gcagcgggac ctgcagatca ctcccaaacg tctggaatat actcgaaaaa
1261aggagaatga gttgtatgaa tcattgatga atattgccaa ccgaaagcag gaggaaatga
1321aggatatgat tgttgagaca cttaatacca tgaaggagga acttctggat gatgctacta
1381acatggagtt taaagacgtc attgtccctg agaatggaga accagtaggc accagagaga
1441tcaaatgctg catccgacag atccaggaac tcatcatctc ccgacttaat caggcagtgg
1501ctaataagct gatcagctca gtggattacc tgagggaaag cttcgtcgga accctggaac
1561gatgtctgca gagcctggag aagtctcagg atgtctcagt tcacatcacc agtaattatc
1621tcaaacagat cttaaatgct gcctatcatg ttgaagtcac gtttcactca gggtcgtcag
1681ttacaaggat gctatgggag caaatcaaac agatcatcca gcgcatcaca tgggtgagcc
1741cacctgccat cactctggaa tggaagagga aggtggccca ggaagccatt gagagcctca
1801gcgcctccaa attggctaag agcatttgca gccaattccg gactcggctc aatagttccc
1861acgaggcttt tgcagcctcc ttgcggcagc tggaagctgg ccactcaggc cggttagaga
1921aaacggaaga tctatggctg agggttcgga aagatcatgc tccccgcctg gcccgccttt
1981ctctggaaag ccgttcttta caggatgtct tgcttcatcg taaacctaaa ctgggacagg
2041aactgggccg gggccagtat ggtgtggtat acctgtgtga caactgggga ggacacttcc
2101cttgtgccct caaatcagtt gtccctccag atgagaagca ctggaatgat ctggctttgg
2161aatttcacta tatgaggtct ctgccgaagc atgagcgatt ggtggatctc catggttcag
2221tcattgacta caactatggt ggtggctcca gcattgctgt gctcctcatt atggagcggc
2281tacaccggga tctctacaca gggctgaagg ctgggctgac cctGGAGACA CGTTTGCAGA
2341TAGCACTAGA TGTGGTGGAG GGAATCCGCT TCCTGCACAG CCAGGGACTT GTCCATCGTG
2401ATATCAAACT GAAAAATGTG CTGCTGGATA AGCAGAACCG TGCCAAGATC ACTGACTTAG
2461GATTCTGCAA GCCAGAGGCC ATGATGTCAG GCAGCATTGT GGGGACACCA ATCCATATGG
2521CCCCTGAACT TTTCACAGGG GCTCGCCCAG AACGTCTTCC TGTGTTTGAT GAGGAGTGCT
2581GGCAGTTGAT GGAAGCCTGT TGGGATGGCG ACCCCTTGAA GAGGCCTCTC TTGGGCATTG
2641TCCAGCCCAT GCTCCAGGGC ATCATGAATC GGCTCTGCAA GTCCAATTCT GAGCAGCCAA
2701ACAGAGGACT AGATGATTCT ACTTAGAACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
2761GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
2821AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAAGTAGCC
2881CTTGGGAAGA AAGCCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
2941aagatt

Download FASTA (ORF) (Full)

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