Construct: ORF TRCN0000491328
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000761.1_s317c1
- Derived from:
- ccsbBroadEn_07573
- DNA Barcode:
- GCAAGTGCCCTCGACGACCACTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC35B1 (10237)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491328
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10237 | SLC35B1 | solute carrier family 35 me... | NM_005827.4 | 99.7% | 99.3% | 8C>T;242G>A |
| 2 | human | 10237 | SLC35B1 | solute carrier family 35 me... | XM_006721632.3 | 81.7% | 81.1% | (many diffs) |
| 3 | human | 10237 | SLC35B1 | solute carrier family 35 me... | NM_001278784.2 | 79% | 76% | 0_1ins176;32_33ins25;41G>A |
| 4 | human | 10237 | SLC35B1 | solute carrier family 35 me... | XM_005256929.3 | 79% | 76% | 0_1ins176;32_33ins25;41G>A |
| 5 | human | 10237 | SLC35B1 | solute carrier family 35 me... | XM_011524180.2 | 79% | 76% | 0_1ins176;32_33ins25;41G>A |
| 6 | human | 10237 | SLC35B1 | solute carrier family 35 me... | XM_011524179.2 | 77.5% | 76.8% | (many diffs) |
| 7 | mouse | 110172 | Slc35b1 | solute carrier family 35, m... | NM_016752.1 | 89.5% | 95.3% | (many diffs) |
| 8 | mouse | 110172 | Slc35b1 | solute carrier family 35, m... | XM_006531985.1 | 62% | 67.7% | (many diffs) |
| 9 | mouse | 110172 | Slc35b1 | solute carrier family 35, m... | XM_006531986.2 | 54.4% | 59.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1032
- ORF length:
- 966
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctttagcagc tccctggtgc ccgaccggct gcgcctgccg ctctgcttcc 121 tgggtgtctt tgtctgctat ttttactatg ggatcctgca ggaaaagata acaagaggaa 181 agtatgggga aggagccaag caggagacgt tcacctttgc cttaactttg gtcttcattc 241 aatgtgtgat caatgctgtg tttgccaaga tcttgatcca gttttttgac actgccaggg 301 tggatcatac ccggagctgg ctctatgctg cctgttctat ctcctatctg ggtgccatgg 361 tctccagcaa ttcagcacta cagtttgtca actacccaac tcaggtcctt ggtaaatcct 421 gcaagccaat cccagtcatg ctccttgggg tgaccctctt gaagaagaag tacccgttgg 481 ccaagtacct gtgtgtgctg ttaattgtgg ctggagtggc ccttttcatg tacaaaccca 541 agaaagttgt tgggatagaa gaacacacag tcggctatgg agagctactc ttgctattat 601 cgctgaccct ggatggactg actggtgttt cccaggacca catgcgggct cattaccaaa 661 caggctccaa ccacatgatg ctgaacatca acctttggtc gacattgctg ctgggaatgg 721 gaatcctgtt cactggGGAG CTCTGGGAGT TCTTGAGCTT TGCTGAAAGG TACCCTGCCA 781 TCATCTATAA CATCCTGCTC TTTGGGCTGA CCAGTGCCCT GGGTCAGAGC TTCATCTTTA 841 TGACGGTTGT GTATTTTGGT CCCCTGACCT GCTCCATCAT CACTACAACT CGAAAGTTCT 901 TCACAATTTT GGCCTCTGTG ATCCTCTTCG CCAATCCCAT CAGCCCCATG CAGTGGGTGG 961 GCACTGTGCT TGTGTTCCTG GGTCTTGGTC TTGATGCCAA GTTTGGGAAA GGAGCTAAGA 1021 AGACATCCCA CTACCCTACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1081 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1141 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCA AGTGCCCTCG ACGACCACTA 1201 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t