Transcript: Mouse XM_006531986.2

PREDICTED: Mus musculus solute carrier family 35, member B1 (Slc35b1), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Slc35b1 (110172)
Length:
1585
CDS:
753..1349

Additional Resources:

NCBI RefSeq record:
XM_006531986.2
NBCI Gene record:
Slc35b1 (110172)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006531986.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000101980 CCCGACCATCATCTATAACAT pLKO.1 1088 CDS 100% 5.625 7.875 N Slc35b1 n/a
2 TRCN0000336379 GTACCCGACCATCATCTATAA pLKO_005 1085 CDS 100% 0.000 0.000 N Slc35b1 n/a
3 TRCN0000101981 CCCAAGAAGGTGGTTGGAATA pLKO.1 852 CDS 100% 10.800 7.560 N Slc35b1 n/a
4 TRCN0000353396 CCCAAGAAGGTGGTTGGAATA pLKO_005 852 CDS 100% 10.800 7.560 N Slc35b1 n/a
5 TRCN0000101984 AGTGACGCTCTTGAAGAAGAA pLKO.1 764 CDS 100% 4.950 3.465 N Slc35b1 n/a
6 TRCN0000336378 AGTGACGCTCTTGAAGAAGAA pLKO_005 764 CDS 100% 4.950 3.465 N Slc35b1 n/a
7 TRCN0000044404 CATCTATAACATCCTGCTCTT pLKO.1 1097 CDS 100% 4.050 2.835 N SLC35B1 n/a
8 TRCN0000101982 CGGTAAATCCTGCAAGCCAAT pLKO.1 725 5UTR 100% 4.050 2.835 N Slc35b1 n/a
9 TRCN0000353332 CGGTAAATCCTGCAAGCCAAT pLKO_005 725 5UTR 100% 4.050 2.835 N Slc35b1 n/a
10 TRCN0000101983 CCACATGATGTTGAACATCAA pLKO.1 986 CDS 100% 0.495 0.347 N Slc35b1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006531986.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07573 pDONR223 100% 54.4% 59.3% None (many diffs) n/a
2 ccsbBroad304_07573 pLX_304 0% 54.4% 59.3% V5 (many diffs) n/a
3 TRCN0000491328 GCAAGTGCCCTCGACGACCACTAC pLX_317 31% 54.4% 59.3% V5 (many diffs) n/a
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