Construct: ORF TRCN0000491344
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019784.2_s317c1
- DNA Barcode:
- AATCGTATTCCGTGCACCCGACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACYP1 (97)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491344
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 97 | ACYP1 | acylphosphatase 1 | NM_001107.5 | 99.6% | 99% | 297_298insG |
| 2 | human | 97 | ACYP1 | acylphosphatase 1 | NM_001302616.2 | 99.6% | 99% | 297_298insG |
| 3 | human | 97 | ACYP1 | acylphosphatase 1 | NM_001302617.2 | 76.5% | 76.1% | 1_90del;387_388insG |
| 4 | human | 97 | ACYP1 | acylphosphatase 1 | NR_126393.2 | 45% | 1_79del;161_239del;456_659delinsG | |
| 5 | human | 97 | ACYP1 | acylphosphatase 1 | NR_126395.2 | 43.1% | (many diffs) | |
| 6 | human | 97 | ACYP1 | acylphosphatase 1 | NR_126394.2 | 37% | (many diffs) | |
| 7 | mouse | 66204 | Acyp1 | acylphosphatase 1, erythroc... | NM_025421.2 | 85.6% | 90% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 372
- ORF length:
- 300
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 CAGGCTTCAC CATGGCAGAA GGAAACACCC TGATATCAGT GGATTATGAA ATTTTTGGGA 121 AGGTGCAAGG GGTGTTTTTC CGTAAGCATA CTCAGGCTGA GGGTAAAAAG CTGGGATTGG 181 TAGGCTGGGT CCAGAACACT GACCGGGGCA CAGTGCAAGG ACAATTGCAA GGTCCCATCT 241 CCAAGGTGCG TCATATGCAG GAATGGCTTG AAACAAGAGG AAGTCCTAAA TCACACATCG 301 ACAAAGCAAA CTTCAACAAT GAAAAAGTCA TCTTGAAGTT GGATTACTCA GACTTCCAAA 361 TTGTAAAAGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 421 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTCGAT 481 TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAATCGTA TTCCGTGCAC CCGACTGACG 541 CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt