Construct: ORF TRCN0000491363

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021465.2_s317c1
DNA Barcode:
CTGATCGGTTGCCGTGTGGTGCGG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PHLDB1 (23187)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491363
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23187 PHLDB1 pleckstrin homology like do... XM_006718803.3 85.5% 56.4% (many diffs)
2 human 23187 PHLDB1 pleckstrin homology like do... NM_001144759.3 85.3% 60.4% (many diffs)
3 human 23187 PHLDB1 pleckstrin homology like do... XM_006718802.3 85% 56% (many diffs)
4 human 23187 PHLDB1 pleckstrin homology like do... XM_006718801.3 84.2% 55.4% (many diffs)
5 human 23187 PHLDB1 pleckstrin homology like do... XM_005271474.5 84.1% 59.4% (many diffs)
6 human 23187 PHLDB1 pleckstrin homology like do... XM_006718800.3 83.1% 54.6% (many diffs)
7 human 23187 PHLDB1 pleckstrin homology like do... XM_006718799.3 82.5% 54.2% (many diffs)
8 human 23187 PHLDB1 pleckstrin homology like do... XM_005271473.3 82.3% 57.9% (many diffs)
9 human 23187 PHLDB1 pleckstrin homology like do... NM_001144758.3 82.2% 57.8% (many diffs)
10 human 23187 PHLDB1 pleckstrin homology like do... NM_015157.4 82.2% 57.8% (many diffs)
11 human 23187 PHLDB1 pleckstrin homology like do... XM_006718798.3 81.8% 53.6% (many diffs)
12 human 23187 PHLDB1 pleckstrin homology like do... XM_005271472.3 81.5% 57.2% (many diffs)
13 human 23187 PHLDB1 pleckstrin homology like do... XM_017017409.1 80.7% 52.8% (many diffs)
14 human 23187 PHLDB1 pleckstrin homology like do... XM_005271471.3 80.5% 56.4% (many diffs)
15 human 23187 PHLDB1 pleckstrin homology like do... XM_017017408.1 80.3% 52.5% (many diffs)
16 human 23187 PHLDB1 pleckstrin homology like do... XM_005271470.3 79.9% 56% (many diffs)
17 human 23187 PHLDB1 pleckstrin homology like do... XM_005271469.3 79.7% 55.8% (many diffs)
18 human 23187 PHLDB1 pleckstrin homology like do... XM_011542710.2 79.5% 51.9% (many diffs)
19 human 23187 PHLDB1 pleckstrin homology like do... XM_005271468.3 79.2% 55.4% (many diffs)
20 human 23187 PHLDB1 pleckstrin homology like do... XM_006718797.3 79.1% 51.6% (many diffs)
21 human 23187 PHLDB1 pleckstrin homology like do... XM_011542708.3 79% 51.6% (many diffs)
22 human 23187 PHLDB1 pleckstrin homology like do... XM_011542709.2 79% 51.6% (many diffs)
23 human 23187 PHLDB1 pleckstrin homology like do... XM_006718796.4 78.9% 51.5% (many diffs)
24 human 23187 PHLDB1 pleckstrin homology like do... XM_011542706.2 78.1% 50.9% (many diffs)
25 human 23187 PHLDB1 pleckstrin homology like do... XM_011542705.2 77.6% 50.6% (many diffs)
26 human 23187 PHLDB1 pleckstrin homology like do... XM_011542704.2 77.4% 50.4% (many diffs)
27 human 23187 PHLDB1 pleckstrin homology like do... XM_011542703.2 76.9% 50.1% (many diffs)
28 human 23187 PHLDB1 pleckstrin homology like do... XM_005277680.3 76.9% 50% (many diffs)
29 human 23187 PHLDB1 pleckstrin homology like do... XM_017017410.1 76.6% 53.3% (many diffs)
30 human 23187 PHLDB1 pleckstrin homology like do... XM_011542707.2 74.5% 51.6% (many diffs)
31 human 23187 PHLDB1 pleckstrin homology like do... XM_017017407.1 74.4% 51.5% (many diffs)
32 human 23187 PHLDB1 pleckstrin homology like do... XM_011542711.2 72% 43.8% (many diffs)
33 human 23187 PHLDB1 pleckstrin homology like do... XM_011542713.2 71.9% 43.7% (many diffs)
34 human 23187 PHLDB1 pleckstrin homology like do... XR_001747798.1 57.3% (many diffs)
35 human 23187 PHLDB1 pleckstrin homology like do... XR_947813.2 55% (many diffs)
36 human 23187 PHLDB1 pleckstrin homology like do... XR_001747797.1 54.7% (many diffs)
37 human 23187 PHLDB1 pleckstrin homology like do... XR_001747799.1 53.5% (many diffs)
38 human 23187 PHLDB1 pleckstrin homology like do... XM_005271475.3 50.7% 92.5% (many diffs)
39 human 23187 PHLDB1 pleckstrin homology like do... XM_017017411.1 50.7% 92.5% (many diffs)
40 human 23187 PHLDB1 pleckstrin homology like do... XM_011542714.2 50.5% 94.5% 1_138del;2544_2545ins2220
41 human 23187 PHLDB1 pleckstrin homology like do... XM_011542715.3 46.4% 14.4% (many diffs)
42 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243973.1 76.4% 53.4% (many diffs)
43 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243972.1 75.6% 52.9% (many diffs)
44 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243970.1 75.2% 52.5% (many diffs)
45 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243978.1 74.7% 56.2% (many diffs)
46 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243969.1 74.2% 51.7% (many diffs)
47 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243976.1 73.6% 55.3% (many diffs)
48 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243961.1 73.5% 49.2% (many diffs)
49 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243967.1 73.5% 51.2% (many diffs)
50 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243975.1 72.9% 54.7% (many diffs)
51 mouse 102693 Phldb1 pleckstrin homology like do... NM_153537.4 72.5% 54.3% (many diffs)
52 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243965.1 72.1% 50.1% (many diffs)
53 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243974.1 72% 53.9% (many diffs)
54 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243964.1 72% 50% (many diffs)
55 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243963.1 71.4% 49.5% (many diffs)
56 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243962.1 71% 49.3% (many diffs)
57 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243971.1 70.8% 52.9% (many diffs)
58 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243960.1 70.6% 48.9% (many diffs)
59 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243957.1 70.1% 48.5% (many diffs)
60 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243958.1 70.1% 48.5% (many diffs)
61 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243956.1 70% 48.4% (many diffs)
62 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243955.1 69.7% 48.2% (many diffs)
63 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243953.1 69.5% 48.1% (many diffs)
64 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243954.1 69.5% 48.1% (many diffs)
65 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243968.1 69.1% 51.4% (many diffs)
66 mouse 102693 Phldb1 pleckstrin homology like do... XM_006509907.3 69.1% 47.7% (many diffs)
67 mouse 102693 Phldb1 pleckstrin homology like do... XM_006509916.3 69.1% 47.7% (many diffs)
68 mouse 102693 Phldb1 pleckstrin homology like do... XM_006509919.4 69.1% 47.7% (many diffs)
69 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243966.1 68.5% 50.8% (many diffs)
70 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243980.1 68% 44.2% (many diffs)
71 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243959.1 67.9% 48.8% (many diffs)
72 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243983.1 67.8% 47.7% (many diffs)
73 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243982.1 65.3% 45.7% (many diffs)
74 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243979.1 64.7% 41.8% (many diffs)
75 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243981.1 64.3% 44.8% (many diffs)
76 mouse 102693 Phldb1 pleckstrin homology like do... XM_006509928.4 62.9% 40.5% (many diffs)
77 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243977.1 62.9% 40.5% (many diffs)
78 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243984.1 48.7% 86.7% (many diffs)
79 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243985.1 47.4% 86.7% (many diffs)
80 mouse 102693 Phldb1 pleckstrin homology like do... XM_006509933.3 39% 7.8% (many diffs)
81 mouse 102693 Phldb1 pleckstrin homology like do... XM_017313091.2 34.8% 6.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2472
ORF length:
2406
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaat
61tcaccatgga cgctctcaat aggaaccaaa taggccctgg atgccagacc cagaccatgg
121tgcagaaagg acccttggac ctgatcgaga caggcaaagg gctgaaagtg caaacggaca
181aaccccacct ggtgagcctg ggcagtgggc gactcagcac agccatcacc ctcctgccgc
241tggaggaagg gaggacggtg attggctctg cagccagaga catctcacta cagggcccag
301gcctggctcc agagcactgc tacatcgaga acctgcgggg caccctcacc ctctacccct
361gtggcaatgc ctgcactatt gatgggctcc ctgtccggca gcctacccgg ctcactcagg
421gctgcatgtt gtgcctgggt cagtccacct tccttcgctt taaccacccg gctgaagcca
481agtggatgaa aagcatgatt ccagcagggg gccgagcccc tgggcccccc tacagccctg
541ttcctgcaga atcagaaagt ctggtaaatg ggaaccacac cccacagact gcaacacggg
601gaccctctgc ctgtgccagc cacagttccc tggtgagctc tattgagaag gacctgcaag
661agatcatgga ctcactggtg ctagaggagc ctggagctgc tggcaagaag cctgccgcaa
721cctctccact gtcaccgatg gctaatggtg ggcgctacct gctgtctccc ccaaccagcc
781ccggcgccat gtctgtgggc tccagctatg agaacacctc tccagccttc tctccactct
841cttcaccagc cagcagtgga agctgtgcca gtcactcacc cagtgggcaa gagccaggac
901cttctgtgcc cccgctggta cctgcccgtt cctccagcta ccatctggcc ctacagcccc
961cacagtcccg cccaagtggt gctcgctccg agagtcctcg gctgagcagg aaagggggcc
1021atgagaggcc tcccagccct ggcctccggg gtctgctgac agacagccct gcagctactg
1081tcttggcgga ggcccggaga gccactgaga gcccccggct gggtgggcag ctgcctgtgg
1141tggccatcag cctgagtgaa tacccagctt ctggtgctct cagtcaaccc accagcattc
1201ctggcagccc caagtttcag cctccagtcc ctgctccccg aaacaagatt ggcacactcc
1261aggaccgccc tcccagccct ttccgtgagc ctccaggcag tgagcgggtg ctaacaacca
1321gcccctcacg ccaactggtg ggccgaacat tttcagatgg gttagccacc cgtaccctgc
1381agcctcctga gagtccccgc ctgggccggc ggggcctgga cagtatgcga gaactacccc
1441ccttaagtcc atctctgtcc cggcgagctc tctccccgct gcccacccgg accaccccag
1501atcccaagct aaacagggaa gtggcagaga gtcctcggcc ccggcgctgg gcagcccatg
1561gggcttcacc agaggacttc tccctgacgc tgggggcacg gggccgtagg acacggagcc
1621cctcacccac actgggtgag tctctggcac cccacaaggg cagcttcagt ggcaggctga
1681gcccagccta cagtctgggc tctcttactg gggcttcacc ctgccagagt ccctgtgtcc
1741agaggaagct ctccagcggg gacttgcggg tgcctgtcac aagggagcgg aaaaatagca
1801tcacagagat cagtgacaat gaggacgacc tcctggagta ccaccggcga cagcgccaag
1861agcggctccg ggagcaggag atggagaggc tggaacgcca gcgcctggag accatcctga
1921acctgtgtgc cgaatacagc cgggctgatg ggggacctga ggctggggag cttcccagca
1981ttggggaggc caccgcagca ttggcactgg caggccggag gccctcacga ggccttgcag
2041gggcctctgg gcggagcagc gaggagcctg gcgttgccac ccaacgccta tgggagagta
2101tggagcgctc agatgaggaa aatctcaagg aggagtgcag cagcactgag agcacccagc
2161aggagcacga agatgcacct agcaccaagc tccagggaga ggtgctagcc ctggaagaag
2221agcgggctca ggtgctgggg cacgtggagc agctcaaggt ccgtgtgaag gagctagagc
2281agcagctgca ggagtcagcc cgagaggccg aaatggagcg ggcactgctg cagggagaga
2341gggaggcaga gcgggcactg ctgcagaagg agcagaaggc agtggatcag ctgcaggaga
2401agctggtggc cttggagaca ggcatccaga aggagaggga caaggaagtg ccaaagcccc
2461ttcttgggca gtgagcacag cccaagggac tgacattggg gagtgattca agttcgggtc
2521ccaccatctg gagaacatct ccctccaagc atgccacaag tgcttacgtt gtgatctttg
2581ggagatggcc ctcgggcctc tgcttccctt gagccttcag gatctgcagc ctcccatccc
2641aggagagggc ggagctggcc gcgggacgga ggcacctgga ggcccgccag gcgctctacg
2701ccgagctcca gacgcagctc gataactgcc ccgagtcagt gcgggaacag ttacaggagc
2761agctgagaag ggtcagttcc accgcccccc ctcccccggc cccgcgggcc acgccccaga
2821aggaagagag gagagcggtg ggacgggacc acctggcctc tgcagtacct gccggacacc
2881agggtcggtg ctgcggtttg gagggaaccg ggaaggttgg ggaatcagcg ctccaggcca
2941tgatgcccgc aaaagggcaa ggccaagtac agcgggagct ggggggaggc aggtttctta
3001cccaatttcc ccgggtccag ccccatacaa cgcatgcccc tgtggcctgg tcttccaggc
3061ccagagtggc attgcaagca ccacccaacc ccctttgtca ctgtcgttga aggaggcaga
3121ggccctggag actgagacaa agctctttga ggacttggag ttccagcagt tggagcggga
3181gagccgcgtg gaggaggagc gcgagctggc cggccagggg ctgctccgga gcaaggctga
3241gctgctccgc agcatcgcca agaggagcgc ctggccatcc tggacagtca ggctgggcag
3301atccgggctc aggccgtgca ggaatcagaa cgcctggccc gggacaagaa tgcctcctta
3361cagctgctgc aaaaggagaa ggagaagctg actgtgctgg aaaggagata ccactcactc
3421acagggggca ggcctttccc gaagaccaca tcgaccctca aagaggttta ccgctccaag
3481atggatggcg aggccaccag cccccttccc cggacccgca gcggccccct cccctcctcc
3541tctggctctt cctcctcctc ctcccagctc agcgtggcta ccctggggcg tagcccctcc
3601ccaaagagcg ctctactcac ccagaatggc acgggcagcc ttcctcgcaa cctggcagcc
3661acactgcagg acatcgagac caagcgccaa ctagctctgc agcagaaggg acaacaagtg
3721attgaagagc agcggcggcg actggctgag ctgaagcaga aagcggcagc tgaggcacag
3781tgccagtggg atgcccttca cggggcagca cccttcccag cgggcccctc gggcttcccc
3841cctctcatgc accactctat cctacaccac ctgcctgcgg ggcgggagcg tggggaggag
3901ggtgagcacg cctatgatac gctgagtctg gagagctctg acagcatgga gaccagcatc
3961tccaccgggg gcaactcggc ctgctcccct gacaacatgt ccagcgcgag tggtctggac
4021atggggaaga tcgaggagat ggagaagatg ctgaaagagg ctcatgcaga gaagaaccgg
4081ctcatggagt cgagggagcg ggaaatggag ctgcggcggc aggccctgga ggaggagcgg
4141cggaggcgtg agcaggtaga acggaggctg cagagtgaga gtgcccggag gcagcagctg
4201gtcgagaagg aggtcaagat gcgggagaaa caattttccc aggcacgacc cctgacccgc
4261tacctgccaa tccggaagga ggactttgac ctgaagacac atattgagtc atcgggccat
4321ggtgttgata cctgcctgca cgtggtgctc agcagcaagg tctgccgtgg ctacttggtc
4381aagatgggcg gcaagattaa atcatggaag aagcgctggt ttgtcttcga ccggctcaag
4441cgcacccttt cctattatgt ggacaagcat gagacgaagc tgaagggagt catctatttc
4501caggccattg aggaagtgta ctacgaccac ctgcgcagtg cagccaagag cccgaaccca
4561gccctcacct tctgcgtaaa gacccatgac cggctgtact acatggtggc cccatctgca
4621gaggccatgc gtatctggat ggatgtcatt gtcaCAGGGG CTGAGGGCTA CACTCAGTTC
4681ATGAACTAAA CTAGTGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC
4741CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT
4801ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTGATCGGT TGCCGTGTGG
4861TGCGGACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)