Construct: ORF TRCN0000491363
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021465.2_s317c1
- DNA Barcode:
- CTGATCGGTTGCCGTGTGGTGCGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PHLDB1 (23187)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491363
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_006718803.3 | 85.5% | 56.4% | (many diffs) |
2 | human | 23187 | PHLDB1 | pleckstrin homology like do... | NM_001144759.3 | 85.3% | 60.4% | (many diffs) |
3 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_006718802.3 | 85% | 56% | (many diffs) |
4 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_006718801.3 | 84.2% | 55.4% | (many diffs) |
5 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_005271474.5 | 84.1% | 59.4% | (many diffs) |
6 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_006718800.3 | 83.1% | 54.6% | (many diffs) |
7 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_006718799.3 | 82.5% | 54.2% | (many diffs) |
8 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_005271473.3 | 82.3% | 57.9% | (many diffs) |
9 | human | 23187 | PHLDB1 | pleckstrin homology like do... | NM_001144758.3 | 82.2% | 57.8% | (many diffs) |
10 | human | 23187 | PHLDB1 | pleckstrin homology like do... | NM_015157.4 | 82.2% | 57.8% | (many diffs) |
11 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_006718798.3 | 81.8% | 53.6% | (many diffs) |
12 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_005271472.3 | 81.5% | 57.2% | (many diffs) |
13 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_017017409.1 | 80.7% | 52.8% | (many diffs) |
14 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_005271471.3 | 80.5% | 56.4% | (many diffs) |
15 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_017017408.1 | 80.3% | 52.5% | (many diffs) |
16 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_005271470.3 | 79.9% | 56% | (many diffs) |
17 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_005271469.3 | 79.7% | 55.8% | (many diffs) |
18 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_011542710.2 | 79.5% | 51.9% | (many diffs) |
19 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_005271468.3 | 79.2% | 55.4% | (many diffs) |
20 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_006718797.3 | 79.1% | 51.6% | (many diffs) |
21 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_011542708.3 | 79% | 51.6% | (many diffs) |
22 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_011542709.2 | 79% | 51.6% | (many diffs) |
23 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_006718796.4 | 78.9% | 51.5% | (many diffs) |
24 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_011542706.2 | 78.1% | 50.9% | (many diffs) |
25 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_011542705.2 | 77.6% | 50.6% | (many diffs) |
26 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_011542704.2 | 77.4% | 50.4% | (many diffs) |
27 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_011542703.2 | 76.9% | 50.1% | (many diffs) |
28 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_005277680.3 | 76.9% | 50% | (many diffs) |
29 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_017017410.1 | 76.6% | 53.3% | (many diffs) |
30 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_011542707.2 | 74.5% | 51.6% | (many diffs) |
31 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_017017407.1 | 74.4% | 51.5% | (many diffs) |
32 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_011542711.2 | 72% | 43.8% | (many diffs) |
33 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_011542713.2 | 71.9% | 43.7% | (many diffs) |
34 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XR_001747798.1 | 57.3% | (many diffs) | |
35 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XR_947813.2 | 55% | (many diffs) | |
36 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XR_001747797.1 | 54.7% | (many diffs) | |
37 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XR_001747799.1 | 53.5% | (many diffs) | |
38 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_005271475.3 | 50.7% | 92.5% | (many diffs) |
39 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_017017411.1 | 50.7% | 92.5% | (many diffs) |
40 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_011542714.2 | 50.5% | 94.5% | 1_138del;2544_2545ins2220 |
41 | human | 23187 | PHLDB1 | pleckstrin homology like do... | XM_011542715.3 | 46.4% | 14.4% | (many diffs) |
42 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243973.1 | 76.4% | 53.4% | (many diffs) |
43 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243972.1 | 75.6% | 52.9% | (many diffs) |
44 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243970.1 | 75.2% | 52.5% | (many diffs) |
45 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243978.1 | 74.7% | 56.2% | (many diffs) |
46 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243969.1 | 74.2% | 51.7% | (many diffs) |
47 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243976.1 | 73.6% | 55.3% | (many diffs) |
48 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243961.1 | 73.5% | 49.2% | (many diffs) |
49 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243967.1 | 73.5% | 51.2% | (many diffs) |
50 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243975.1 | 72.9% | 54.7% | (many diffs) |
51 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | NM_153537.4 | 72.5% | 54.3% | (many diffs) |
52 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243965.1 | 72.1% | 50.1% | (many diffs) |
53 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243974.1 | 72% | 53.9% | (many diffs) |
54 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243964.1 | 72% | 50% | (many diffs) |
55 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243963.1 | 71.4% | 49.5% | (many diffs) |
56 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243962.1 | 71% | 49.3% | (many diffs) |
57 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243971.1 | 70.8% | 52.9% | (many diffs) |
58 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243960.1 | 70.6% | 48.9% | (many diffs) |
59 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243957.1 | 70.1% | 48.5% | (many diffs) |
60 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243958.1 | 70.1% | 48.5% | (many diffs) |
61 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243956.1 | 70% | 48.4% | (many diffs) |
62 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243955.1 | 69.7% | 48.2% | (many diffs) |
63 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243953.1 | 69.5% | 48.1% | (many diffs) |
64 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243954.1 | 69.5% | 48.1% | (many diffs) |
65 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243968.1 | 69.1% | 51.4% | (many diffs) |
66 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_006509907.3 | 69.1% | 47.7% | (many diffs) |
67 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_006509916.3 | 69.1% | 47.7% | (many diffs) |
68 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_006509919.4 | 69.1% | 47.7% | (many diffs) |
69 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243966.1 | 68.5% | 50.8% | (many diffs) |
70 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243980.1 | 68% | 44.2% | (many diffs) |
71 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243959.1 | 67.9% | 48.8% | (many diffs) |
72 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243983.1 | 67.8% | 47.7% | (many diffs) |
73 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243982.1 | 65.3% | 45.7% | (many diffs) |
74 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243979.1 | 64.7% | 41.8% | (many diffs) |
75 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243981.1 | 64.3% | 44.8% | (many diffs) |
76 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_006509928.4 | 62.9% | 40.5% | (many diffs) |
77 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243977.1 | 62.9% | 40.5% | (many diffs) |
78 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243984.1 | 48.7% | 86.7% | (many diffs) |
79 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_030243985.1 | 47.4% | 86.7% | (many diffs) |
80 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_006509933.3 | 39% | 7.8% | (many diffs) |
81 | mouse | 102693 | Phldb1 | pleckstrin homology like do... | XM_017313091.2 | 34.8% | 6.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2472
- ORF length:
- 2406
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaat 61 tcaccatgga cgctctcaat aggaaccaaa taggccctgg atgccagacc cagaccatgg 121 tgcagaaagg acccttggac ctgatcgaga caggcaaagg gctgaaagtg caaacggaca 181 aaccccacct ggtgagcctg ggcagtgggc gactcagcac agccatcacc ctcctgccgc 241 tggaggaagg gaggacggtg attggctctg cagccagaga catctcacta cagggcccag 301 gcctggctcc agagcactgc tacatcgaga acctgcgggg caccctcacc ctctacccct 361 gtggcaatgc ctgcactatt gatgggctcc ctgtccggca gcctacccgg ctcactcagg 421 gctgcatgtt gtgcctgggt cagtccacct tccttcgctt taaccacccg gctgaagcca 481 agtggatgaa aagcatgatt ccagcagggg gccgagcccc tgggcccccc tacagccctg 541 ttcctgcaga atcagaaagt ctggtaaatg ggaaccacac cccacagact gcaacacggg 601 gaccctctgc ctgtgccagc cacagttccc tggtgagctc tattgagaag gacctgcaag 661 agatcatgga ctcactggtg ctagaggagc ctggagctgc tggcaagaag cctgccgcaa 721 cctctccact gtcaccgatg gctaatggtg ggcgctacct gctgtctccc ccaaccagcc 781 ccggcgccat gtctgtgggc tccagctatg agaacacctc tccagccttc tctccactct 841 cttcaccagc cagcagtgga agctgtgcca gtcactcacc cagtgggcaa gagccaggac 901 cttctgtgcc cccgctggta cctgcccgtt cctccagcta ccatctggcc ctacagcccc 961 cacagtcccg cccaagtggt gctcgctccg agagtcctcg gctgagcagg aaagggggcc 1021 atgagaggcc tcccagccct ggcctccggg gtctgctgac agacagccct gcagctactg 1081 tcttggcgga ggcccggaga gccactgaga gcccccggct gggtgggcag ctgcctgtgg 1141 tggccatcag cctgagtgaa tacccagctt ctggtgctct cagtcaaccc accagcattc 1201 ctggcagccc caagtttcag cctccagtcc ctgctccccg aaacaagatt ggcacactcc 1261 aggaccgccc tcccagccct ttccgtgagc ctccaggcag tgagcgggtg ctaacaacca 1321 gcccctcacg ccaactggtg ggccgaacat tttcagatgg gttagccacc cgtaccctgc 1381 agcctcctga gagtccccgc ctgggccggc ggggcctgga cagtatgcga gaactacccc 1441 ccttaagtcc atctctgtcc cggcgagctc tctccccgct gcccacccgg accaccccag 1501 atcccaagct aaacagggaa gtggcagaga gtcctcggcc ccggcgctgg gcagcccatg 1561 gggcttcacc agaggacttc tccctgacgc tgggggcacg gggccgtagg acacggagcc 1621 cctcacccac actgggtgag tctctggcac cccacaaggg cagcttcagt ggcaggctga 1681 gcccagccta cagtctgggc tctcttactg gggcttcacc ctgccagagt ccctgtgtcc 1741 agaggaagct ctccagcggg gacttgcggg tgcctgtcac aagggagcgg aaaaatagca 1801 tcacagagat cagtgacaat gaggacgacc tcctggagta ccaccggcga cagcgccaag 1861 agcggctccg ggagcaggag atggagaggc tggaacgcca gcgcctggag accatcctga 1921 acctgtgtgc cgaatacagc cgggctgatg ggggacctga ggctggggag cttcccagca 1981 ttggggaggc caccgcagca ttggcactgg caggccggag gccctcacga ggccttgcag 2041 gggcctctgg gcggagcagc gaggagcctg gcgttgccac ccaacgccta tgggagagta 2101 tggagcgctc agatgaggaa aatctcaagg aggagtgcag cagcactgag agcacccagc 2161 aggagcacga agatgcacct agcaccaagc tccagggaga ggtgctagcc ctggaagaag 2221 agcgggctca ggtgctgggg cacgtggagc agctcaaggt ccgtgtgaag gagctagagc 2281 agcagctgca ggagtcagcc cgagaggccg aaatggagcg ggcactgctg cagggagaga 2341 gggaggcaga gcgggcactg ctgcagaagg agcagaaggc agtggatcag ctgcaggaga 2401 agctggtggc cttggagaca ggcatccaga aggagaggga caaggaagtg ccaaagcccc 2461 ttcttgggca gtgagcacag cccaagggac tgacattggg gagtgattca agttcgggtc 2521 ccaccatctg gagaacatct ccctccaagc atgccacaag tgcttacgtt gtgatctttg 2581 ggagatggcc ctcgggcctc tgcttccctt gagccttcag gatctgcagc ctcccatccc 2641 aggagagggc ggagctggcc gcgggacgga ggcacctgga ggcccgccag gcgctctacg 2701 ccgagctcca gacgcagctc gataactgcc ccgagtcagt gcgggaacag ttacaggagc 2761 agctgagaag ggtcagttcc accgcccccc ctcccccggc cccgcgggcc acgccccaga 2821 aggaagagag gagagcggtg ggacgggacc acctggcctc tgcagtacct gccggacacc 2881 agggtcggtg ctgcggtttg gagggaaccg ggaaggttgg ggaatcagcg ctccaggcca 2941 tgatgcccgc aaaagggcaa ggccaagtac agcgggagct ggggggaggc aggtttctta 3001 cccaatttcc ccgggtccag ccccatacaa cgcatgcccc tgtggcctgg tcttccaggc 3061 ccagagtggc attgcaagca ccacccaacc ccctttgtca ctgtcgttga aggaggcaga 3121 ggccctggag actgagacaa agctctttga ggacttggag ttccagcagt tggagcggga 3181 gagccgcgtg gaggaggagc gcgagctggc cggccagggg ctgctccgga gcaaggctga 3241 gctgctccgc agcatcgcca agaggagcgc ctggccatcc tggacagtca ggctgggcag 3301 atccgggctc aggccgtgca ggaatcagaa cgcctggccc gggacaagaa tgcctcctta 3361 cagctgctgc aaaaggagaa ggagaagctg actgtgctgg aaaggagata ccactcactc 3421 acagggggca ggcctttccc gaagaccaca tcgaccctca aagaggttta ccgctccaag 3481 atggatggcg aggccaccag cccccttccc cggacccgca gcggccccct cccctcctcc 3541 tctggctctt cctcctcctc ctcccagctc agcgtggcta ccctggggcg tagcccctcc 3601 ccaaagagcg ctctactcac ccagaatggc acgggcagcc ttcctcgcaa cctggcagcc 3661 acactgcagg acatcgagac caagcgccaa ctagctctgc agcagaaggg acaacaagtg 3721 attgaagagc agcggcggcg actggctgag ctgaagcaga aagcggcagc tgaggcacag 3781 tgccagtggg atgcccttca cggggcagca cccttcccag cgggcccctc gggcttcccc 3841 cctctcatgc accactctat cctacaccac ctgcctgcgg ggcgggagcg tggggaggag 3901 ggtgagcacg cctatgatac gctgagtctg gagagctctg acagcatgga gaccagcatc 3961 tccaccgggg gcaactcggc ctgctcccct gacaacatgt ccagcgcgag tggtctggac 4021 atggggaaga tcgaggagat ggagaagatg ctgaaagagg ctcatgcaga gaagaaccgg 4081 ctcatggagt cgagggagcg ggaaatggag ctgcggcggc aggccctgga ggaggagcgg 4141 cggaggcgtg agcaggtaga acggaggctg cagagtgaga gtgcccggag gcagcagctg 4201 gtcgagaagg aggtcaagat gcgggagaaa caattttccc aggcacgacc cctgacccgc 4261 tacctgccaa tccggaagga ggactttgac ctgaagacac atattgagtc atcgggccat 4321 ggtgttgata cctgcctgca cgtggtgctc agcagcaagg tctgccgtgg ctacttggtc 4381 aagatgggcg gcaagattaa atcatggaag aagcgctggt ttgtcttcga ccggctcaag 4441 cgcacccttt cctattatgt ggacaagcat gagacgaagc tgaagggagt catctatttc 4501 caggccattg aggaagtgta ctacgaccac ctgcgcagtg cagccaagag cccgaaccca 4561 gccctcacct tctgcgtaaa gacccatgac cggctgtact acatggtggc cccatctgca 4621 gaggccatgc gtatctggat ggatgtcatt gtcaCAGGGG CTGAGGGCTA CACTCAGTTC 4681 ATGAACTAAA CTAGTGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC 4741 CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT 4801 ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTGATCGGT TGCCGTGTGG 4861 TGCGGACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt