Construct: ORF TRCN0000491368

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010614.1_s317c1
Derived from:
ccsbBroadEn_01612
DNA Barcode:
GTCATTTTCATGCAGCTTGACGAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
STAU1 (6780)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491368
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6780 STAU1 staufen double-stranded RNA... NM_001319135.1 100% 100%
2 human 6780 STAU1 staufen double-stranded RNA... NM_001322929.1 100% 100%
3 human 6780 STAU1 staufen double-stranded RNA... NM_001322930.1 100% 100%
4 human 6780 STAU1 staufen double-stranded RNA... NM_017453.3 100% 100%
5 human 6780 STAU1 staufen double-stranded RNA... NM_001322932.1 98.9% 98.9% 608_625del
6 human 6780 STAU1 staufen double-stranded RNA... NM_001322933.1 98.9% 98.9% 608_625del
7 human 6780 STAU1 staufen double-stranded RNA... XM_006723865.1 98.9% 98.9% 608_625del
8 human 6780 STAU1 staufen double-stranded RNA... NM_001322931.1 91.3% 88.4% (many diffs)
9 human 6780 STAU1 staufen double-stranded RNA... XM_017028028.1 91.3% 88.4% (many diffs)
10 human 6780 STAU1 staufen double-stranded RNA... NM_001322928.1 90.3% 87.5% (many diffs)
11 human 6780 STAU1 staufen double-stranded RNA... XM_011529015.1 90.3% 87.5% (many diffs)
12 human 6780 STAU1 staufen double-stranded RNA... NM_004602.3 85.9% 85.9% 0_1ins243
13 human 6780 STAU1 staufen double-stranded RNA... NM_017452.3 85.9% 85.9% 0_1ins243
14 human 6780 STAU1 staufen double-stranded RNA... NM_017454.3 85.9% 85.9% 0_1ins243
15 human 6780 STAU1 staufen double-stranded RNA... XM_024451973.1 85.9% 85.9% 0_1ins243
16 human 6780 STAU1 staufen double-stranded RNA... NM_001037328.2 85% 85% 0_1ins243;365_382del
17 human 6780 STAU1 staufen double-stranded RNA... NM_001319134.1 85% 85% 0_1ins243;365_382del
18 human 6780 STAU1 staufen double-stranded RNA... NM_001322927.1 85% 85% 0_1ins243;365_382del
19 human 6780 STAU1 staufen double-stranded RNA... XM_005260527.1 85% 85% 0_1ins243;365_382del
20 mouse 20853 Stau1 staufen (RNA binding protei... NM_001109905.2 73.8% 77.4% (many diffs)
21 mouse 20853 Stau1 staufen (RNA binding protei... NM_011490.4 73.3% 77.2% (many diffs)
22 mouse 20853 Stau1 staufen (RNA binding protei... NM_001291160.1 73.1% 76.7% (many diffs)
23 mouse 20853 Stau1 staufen (RNA binding protei... NM_001109906.2 73% 76.6% (many diffs)
24 mouse 20853 Stau1 staufen (RNA binding protei... XM_006499071.1 72.4% 75.9% (many diffs)
25 mouse 20853 Stau1 staufen (RNA binding protei... XM_006499072.1 72.4% 75.9% (many diffs)
26 mouse 20853 Stau1 staufen (RNA binding protei... XM_011239406.1 65.9% 68.7% (many diffs)
27 mouse 20853 Stau1 staufen (RNA binding protei... NR_111895.1 34.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1797
ORF length:
1731
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc tcaagttcaa gtgcaagttc agaacccatc tgctgctctc tcagggagcc
121aaatactgaa caagaaccag tctcttctct cacagccttt gatgagtatt ccttctacta
181ctagctctct gccctctgaa aatgcaggta gacccattca aaactctgct ttaccctctg
241catctattac atccaccagt gcagctgcag aaagcataac ccctactgta gaactaaatg
301cactgtgcat gaaacttgga aaaaaaccaa tgtataagcc tgttgaccct tactctcgga
361tgcagtccac ctataactac aacatgagag gaggtgctta tcccccgagg tacttttacc
421catttccagt tccaccttta ctttatcaag tggaactttc tgtgggagga cagcaattta
481atggcaaagg aaagacaaga caggctgcga aacacgatgc tgctgccaaa gcgttgagga
541tcctgcagaa tgagcccctg ccagagaggc tggaggtgaa tggaagagaa tccgaagaag
601aaaatctcaa taaatctgaa ataagtcaag tgtttgagat tgcacttaaa cggaacttgc
661ctgtgaattt cgaggtggcc cgggagagtg gcccacccca catgaagaac tttgtgacca
721aggtttcggt tggggagttt gtgggggaag gtgaagggaa aagcaagaag atttcaaaga
781aaaatgccgc catagctgtt cttgaggagc tgaagaagtt accgcccctg cctgcagttg
841aacgagtaaa gcctagaatc aaaaagaaaa caaaacccat agtcaagcca cagacaagcc
901cagaatatgg ccaggggatc aatccgatta gccgactggc ccagatccag caggcaaaaa
961aggagaagga gccagagtac acgctcctca cagagcgagg cctcccgcgc cgcagggagt
1021ttgtgatgca ggtgaaggtt ggaaaccaca ctgcagaagg aacgggcacc aacaagaagg
1081tggccaagcg caatgcagcc gagaacatgc tggagatcct tggtttcaaa gtcccgcagg
1141cgcagcccac caaacccgca ctcaagtcag aggagaagac acccataaag aaaccagggg
1201atggaagaaa agtaaccttt tttgaacctg gctctgggga tgaaaatggg actagtaata
1261aagaggatga gttcaggatg ccttatctaa gtcatcagca gctgcctgct ggaattcttc
1321ccatggtgcc cgaggtcgcc caggctgtag gagttagtca aggacatcac accaaagatt
1381ttaccagggc agctccgaat cctgccaagg ccacggtaac tgccatgata gcccgagagt
1441tgttgtatgg gggcaccTCG CCCACAGCCG AGACCATTTT AAAGAATAAC ATCTCTTCAG
1501GCCACGTACC CCATGGACCT CTCACGAGAC CCTCTGAGCA ACTGGACTAT CTTTCCAGAG
1561TCCAGGGATT CCAGGTTGAA TACAAAGACT TCCCCAAAAA CAACAAGAAC GAATTTGTAT
1621CTCTTATCAA TTGCTCCTCT CAGCCACCTC TGATCAGCCA TGGTATCGGC AAGGATGTGG
1681AGTCCTGCCA TGATATGGCT GCGCTGAACA TCTTAAAGTT GCTGTCTGAG TTGGACCAAC
1741AAAGTACAGA GATGCCAAGA ACAGGAAACG GACCAATGTC TGTGTGTGGG AGGTGCTGCC
1801CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1861TCGATTCTAC GTAGTAATGA ACTAGTCCGT AATTGAAAGT ATTTCGATTT CTTGGCTTTA
1921TATATCTTGT GGAAAGGACG AGTCATTTTC ATGCAGCTTG ACGAAACGCG TTAAGTCgac
1981aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)