Construct: ORF TRCN0000491413
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021791.2_s317c1
- DNA Barcode:
- CTGCATACTTAGTCTAAACTTGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NUMB (8650)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491413
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001005745.1 | 100% | 100% | |
| 2 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001005744.1 | 98.1% | 98.1% | 202_234del |
| 3 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001320114.1 | 98.1% | 98.1% | 202_234del |
| 4 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_003744.5 | 92.5% | 92.5% | 1063_1206del |
| 5 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001005743.1 | 90.9% | 90.9% | 202_234del;1096_1239del |
| 6 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_001272056.1 | 88.9% | 94% | (many diffs) |
| 7 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_010949.2 | 87.3% | 92.3% | (many diffs) |
| 8 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_001272055.1 | 82.3% | 86.9% | (many diffs) |
| 9 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515578.1 | 82.3% | 86.9% | (many diffs) |
| 10 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_001136075.2 | 81% | 85.4% | (many diffs) |
| 11 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515576.1 | 81% | 85.4% | (many diffs) |
| 12 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515577.2 | 81% | 85.4% | (many diffs) |
| 13 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_011244012.2 | 81% | 85.4% | (many diffs) |
| 14 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515575.2 | 77.5% | 82% | (many diffs) |
| 15 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515574.2 | 76.3% | 80.7% | (many diffs) |
| 16 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515573.2 | 72.5% | 76.5% | (many diffs) |
| 17 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515572.2 | 71.4% | 75.4% | (many diffs) |
| 18 | mouse | 18222 | Numb | numb homolog (Drosophila) | NR_073563.1 | 44.6% | (many diffs) | |
| 19 | mouse | 18222 | Numb | numb homolog (Drosophila) | NR_073564.1 | 42.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1845
- ORF length:
- 1776
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gaacaaatta cggcaaagtt ttaggagaaa gaaggatgtt tatgttccag 121 aggccagtcg tccacatcag tggcagacag atgaagaagg cgttcgcacc ggaaaatgta 181 gcttcccggt taagtacctt ggccatgtag aagttgatga atcaagagga atgcacatct 241 gtgaagatgc tgtaaaaaga ttgaaagcta ctggaaagaa agcagttaaa gcagttctgt 301 gggtctcagc agatggactc agagttgtgg atgaaaaaac taaggacctc atagttgacc 361 agacgataga gaaagtttct ttctgtgccc cagacaggaa ctttgataga gccttttctt 421 acatatgccg tgatggcacc actcgtcgct ggatctgtca ctgcttcatg gctgtcaagg 481 acacaggtga aaggttgagc catgcagtag gctgtgcttt tgcagcctgt ttagagcgca 541 agcagaagcg ggagaaggaa tgtggagtga ctgctacttt tgatgctagt cggaccactt 601 ttacaagaga aggatcattc cgtgtcacaa cagccactga acaagcagaa agagaggaga 661 tcatgaaaca aatgcaagat gccaagaaag ctgaaacaga taagatagtc gttggttcat 721 cagttgcccc tggcaacact gccccatccc catcctctcc cacctctcct acttctgatg 781 ccacgacctc tctggagatg aacaatcctc atgccatccc acgccggcat gctccaattg 841 aacagcttgc tcgccaaggc tctttccgag gttttcctgc tcttagccag aagatgtcac 901 cctttaaacg ccaactatcc ctacgcatca atgagttgcc ttccactatg cagaggaaga 961 ctgatttccc cattaaaaat gcagtgccag aagtagaagg ggaggcagag agcatcagct 1021 ccctgtgctc acagatcacc aatgccttca gcacacctga ggaccccttc tcatctgctc 1081 cgatgaccaa accagtgaca gtggtggcac cacaatctcc taccttccaa gggaccgagt 1141 ggggtcaatc ttctggtgct gcctctccag gtctcttcca ggccggtcat agacgtactc 1201 cctctgaggc cgaccgatgg ttagaagagg tgtctaagag cgtccgggct cagcagcccc 1261 aggcctcagc tgctcctctg cagccagttc tccagcctcc tccacccact gccatctccc 1321 agccagcatc acctttccaa gggaatgcat tcctcacctc tcagcctgtg ccagtgggtg 1381 tggtcccagc cctgcaacca gcctttgtcc ctgcccagtc ctatcctgtg gccaatggaa 1441 tgccctatcc agcccctaat gtgcctgtgg tgggcatcac tccctcccag atggtggcca 1501 acgtatttgg cactgcaggc caccctcagg ctgcccatcc ccatcagtca cccagcctgg 1561 tcaggcagca gacattccct cactacgagg caagcagtgc taccaccagt cccttcttta 1621 agccTCCTGC TCAGCACCTC AACGGTTCTG CAGCTTTCAA TGGTGTAGAT GATGGCAGGT 1681 TGGCCTCAGC AGACAGGCAT ACAGAGGTTC CTACAGGCAC CTGCCCAGTG GATCCTTTTG 1741 AAGCCCAGTG GGCTGCATTA GAAAATAAGT CCAAGCAGCG TACTAATCCC TCCCCTACCA 1801 ACCCTTTCTC CAGTGACTTA CAGAAGACGT TTGAAATTGA ACTTTAAGAC CCAGCTTTCT 1861 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1921 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1981 GTGGAAAGGA CGACTGCATA CTTAGTCTAA ACTTGTCACG CGTTAAGTCg acaatcaacc 2041 tctggattac aaaatttgtg aaagatt