Construct: ORF TRCN0000491416
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000201.1_s317c1
- Derived from:
- ccsbBroadEn_06343
- DNA Barcode:
- CGAAAAGATGCCAAGAGCTTAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GUCY1B1 (2983)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491416
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2983 | GUCY1B1 | guanylate cyclase 1 soluble... | NM_000857.4 | 99.9% | 100% | 1047G>A |
| 2 | human | 2983 | GUCY1B1 | guanylate cyclase 1 soluble... | NM_001291951.2 | 96.5% | 96.5% | 298_363del;1113G>A |
| 3 | human | 2983 | GUCY1B1 | guanylate cyclase 1 soluble... | NM_001291952.2 | 96.4% | 95.7% | (many diffs) |
| 4 | human | 2983 | GUCY1B1 | guanylate cyclase 1 soluble... | NM_001291954.2 | 94.6% | 94.6% | 1047G>A;1175_1176ins99 |
| 5 | human | 2983 | GUCY1B1 | guanylate cyclase 1 soluble... | XM_011531901.1 | 93.4% | 93.5% | 1047G>A;1176_1304del |
| 6 | human | 2983 | GUCY1B1 | guanylate cyclase 1 soluble... | NM_001291955.2 | 88.9% | 89% | 0_1ins204;843G>A |
| 7 | human | 2983 | GUCY1B1 | guanylate cyclase 1 soluble... | XM_017008132.1 | 88.9% | 89% | 0_1ins204;843G>A |
| 8 | human | 2983 | GUCY1B1 | guanylate cyclase 1 soluble... | XM_017008133.1 | 88.9% | 89% | 0_1ins204;843G>A |
| 9 | human | 2983 | GUCY1B1 | guanylate cyclase 1 soluble... | NM_001291953.2 | 83.1% | 83.2% | 0_1ins204;843G>A;972_1100del |
| 10 | human | 2983 | GUCY1B1 | guanylate cyclase 1 soluble... | XM_017008130.1 | 83.1% | 83.2% | 0_1ins204;843G>A;972_1100del |
| 11 | human | 2983 | GUCY1B1 | guanylate cyclase 1 soluble... | XM_017008131.2 | 83.1% | 83.2% | 0_1ins204;843G>A;972_1100del |
| 12 | mouse | 54195 | Gucy1b3 | guanylate cyclase 1, solubl... | NM_017469.4 | 88.5% | 98.7% | (many diffs) |
| 13 | mouse | 54195 | Gucy1b3 | guanylate cyclase 1, solubl... | NM_001161796.1 | 87.3% | 92.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1923
- ORF length:
- 1857
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta cggatttgtg aatcacgccc tggagttgct ggtgatccgc aattacggcc 121 ccgaggtgtg ggaagacatc aaaaaagagg cacagttaga tgaagaagga cagtttcttg 181 tcagaataat atatgatgac tccaaaactt atgatttggt tgctgctgca agcaaagtcc 241 tcaatctcaa tgctggagaa atcctccaaa tgtttgggaa gatgtttttc gtcttttgcc 301 aagaatctgg ttatgataca atcttgcgtg tcctgggctc taatgtcaga gaatttctac 361 agaaccttga tgctctgcac gaccaccttg ctaccatcta cccaggaatg cgtgcacctt 421 cctttaggtg cactgatgca gaaaagggca aaggactcat tttgcactac tactcagaga 481 gagaaggact tcaggatatt gtcattggaa tcatcaaaac agtggcacaa caaatccatg 541 gcactgaaat agacatgaag gttattcagc aaagaaatga agaatgtgat catactcaat 601 ttttaattga agaaaaagag tcaaaagaag aggattttta tgaagatctt gacagatttg 661 aagaaaatgg tacccaggaa tcacgcatca gcccatatac attctgcaaa gcttttcctt 721 ttcatataat atttgaccgg gacctagtgg tcactcagtg tggcaatgct atatacagag 781 ttctccccca gctccagcct gggaattgca gccttctgtc tgtcttctcg ctggttcgtc 841 ctcatattga tattagtttc catgggatcc tttctcacat caatactgtt tttgtattga 901 gaagcaagga aggattgttg gatgtggaga aattagaatg tgaggatgaa ctgactggga 961 ctgagatcag ctgcttacgt ctcaagggtc aaatgatcta cttacctgaa gcagatagca 1021 tactttttct atgttcacca agtgtcatga acctggacga tttgacaagg agagggctgt 1081 atctaagtga catccctctg catgatgcca cacgcgatct tgttcttttg ggagaacaat 1141 ttagagagga atacaaactc acccaagaac tggaaatcct cactgacagg ctacagctca 1201 cgttaagagc cctggaagat gaaaagaaaa agacagacac attgctgtat tctgtccttc 1261 ctccgtctgt tgccaatgag ctgcggcaca agcgtccagt gcctgccaaa agatatgaca 1321 atgtgaccat cctctttagt ggcattgtgg gcttcaatgc tttctgtagc aagcatgcat 1381 ctggagaagg agccatgaag atcgtcaacc tcctcaacga cctctacacc agatttgaca 1441 cactgactga ttcccggaaa aacccatttg tttataaggt ggagactgtt ggtgacaagt 1501 atatgacagt gagtggttta ccagagccat gcattcacca tgcacgatcc atctgccacc 1561 tggccttgga catgatGGAA ATTGCTGGCC AGGTTCAAGT AGATGGTGAA TCTGTTCAGA 1621 TAACAATAGG GATACACACT GGAGAGGTAG TTACAGGTGT CATAGGACAG CGGATGCCTC 1681 GATACTGTCT TTTTGGGAAT ACTGTCAACC TCACAAGCCG AACAGAAACC ACAGGAGAAA 1741 AGGGAAAAAT AAATGTGTCT GAATATACAT ACAGATGTCT TATGTCTCCA GAAAATTCAG 1801 ATCCACAATT CCACTTGGAG CACAGAGGCC CAGTGTCCAT GAAGGGCAAA AAAGAACCAA 1861 TGCAAGTTTG GTTTCTATCC AGAAAAAATA CAGGAACAGA GGAAACAAAG CAGGATGATG 1921 ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1981 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2041 GGCTTTATAT ATCTTGTGGA AAGGACGACG AAAAGATGCC AAGAGCTTAA TCACGCGTTA 2101 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt