Construct: ORF TRCN0000491419
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019169.2_s317c1
- DNA Barcode:
- TATCAGGTTCTGCCAGGCCGGAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APLNR (187)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491419
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 187 | APLNR | apelin receptor | NM_005161.4 | 100% | 100% | |
| 2 | human | 187 | APLNR | apelin receptor | NR_027991.2 | 66% | 1_246del;1387_1727del | |
| 3 | mouse | 23796 | Aplnr | apelin receptor | NM_011784.3 | 86.9% | 91.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1215
- ORF length:
- 1140
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggag gaaggtggtg attttgacaa ctactatggg gcagacaacc 121 agtctgagtg tgagtacaca gactggaaat cctcgggggc cctcatccct gccatctaca 181 tgttggtctt cctcctgggc accacgggca acggtctggt gctctggacc gtgtttcgga 241 gcagccggga gaagaggcgc tcagctgata tcttcattgc tagcctggcg gtggctgacc 301 tgaccttcgt ggtgacgctg cccctgtggg ctacctacac gtaccgggac tatgactggc 361 cctttgggac cttcttctgc aagctcagca gctacctcat cttcgtcaac atgtacgcca 421 gcgtcttctg cctcaccggc ctcagcttcg accgctacct ggccatcgtg aggccagtgg 481 ccaatgctcg gctgaggctg cgggtcagcg gggccgtggc cacggcagtt ctttgggtgc 541 tggccgccct cctggccatg cctgtcatgg tgttacgcac caccggggac ttggagaaca 601 ccactaaggt gcagtgctac atggactact ccatggtggc cactgtgagc tcagagtggg 661 cctgggaggt gggccttggg gtctcgtcca ccaccgtggg ctttgtggtg cccttcacca 721 tcatgctgac ctgttacttc ttcatcgccc aaaccatcgc tggccacttc cgcaaggaac 781 gcatcgaggg cctgcggaag cggcgccggc tgctcagcat catcgtggtg ctggtggtga 841 cctttgccct gtgctggatg ccctaccacc tggtgaagac gctgtacatg ctgggcagcc 901 tgctgcactg gccctgtgac tttgaccTCT TCCTCATGAA CATCTTCCCC TACTGCACCT 961 GCATCAGCTA CGTCAACAGC TGCCTCAACC CCTTCCTCTA TGCCTTTTTC GACCCCCGCT 1021 TCCGCCAGGC CTGCACCTCC ATGCTCTGCT GTGGCCAGAG CAGGTGCGCA GGCACCTCCC 1081 ACAGCAGCAG TGGGGAGAAG TCAGCCAGCT ACTCTTCGGG GCACAGCCAG GGGCCCGGCC 1141 CCAACATGGG CAAGGGTGGA GAACAGATGC ACGAGAAATC CATCCCCTAC AGCCAGGAGA 1201 CCCTTGTGGT TGACGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1261 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1321 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TATCAGGTTC TGCCAGGCCG 1381 GAGCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt