Construct: ORF TRCN0000491483
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005993.1_s317c1
- Derived from:
- ccsbBroadEn_01082
- DNA Barcode:
- CATGTCGAAACAGCCTCAAGAGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NEDD8 (4738)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491483
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4738 | NEDD8 | NEDD8 ubiquitin like modifier | NM_006156.3 | 100% | 100% | |
| 2 | human | 100528064 | NEDD8-MDP1 | NEDD8-MDP1 readthrough | NM_001199823.2 | 36.1% | 28.4% | (many diffs) |
| 3 | human | 100528064 | NEDD8-MDP1 | NEDD8-MDP1 readthrough | NR_137631.1 | 34.7% | (many diffs) | |
| 4 | human | 100528064 | NEDD8-MDP1 | NEDD8-MDP1 readthrough | NR_137630.1 | 34% | (many diffs) | |
| 5 | human | 100528064 | NEDD8-MDP1 | NEDD8-MDP1 readthrough | NR_137632.1 | 24.1% | (many diffs) | |
| 6 | mouse | 18002 | Nedd8 | neural precursor cell expre... | NM_008683.3 | 92.6% | 98.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 312
- ORF length:
- 243
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GCTAATTAAA GTGAAGACGC TGACCGGAAA GGAGATTGAG ATTGACATTG 121 AACCTACAGA CAAGGTGGAG CGAATCAAGG AGCGTGTGGA GGAGAAAGAG GGAATCCCCC 181 CACAACAGCA GAGGCTCATC TACAGTGGCA AGCAGATGAA TGATGAGAAG ACAGCAGCTG 241 ATTACAAGAT TTTAGGTGGT TCAGTCCTTC ACCTGGTGTT GGCTCTGAGA GGAGGAGGTG 301 GTCTTAGGCA GTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 361 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 421 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAT GTCGAAACAG CCTCAAGAGA 481 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t