Transcript: Mouse NM_008683.3

Mus musculus neural precursor cell expressed, developmentally down-regulated gene 8 (Nedd8), mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Nedd8 (18002)
Length:
602
CDS:
95..340

Additional Resources:

NCBI RefSeq record:
NM_008683.3
NBCI Gene record:
Nedd8 (18002)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_008683.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000284919 GAAACTTGGTTCCGTTTACCT pLKO_005 342 3UTR 100% 3.000 4.200 N Nedd8 n/a
2 TRCN0000012741 CAAGCAAATGAATGATGAGAA pLKO.1 235 CDS 100% 4.950 3.465 N Nedd8 n/a
3 TRCN0000273011 CAAGCAAATGAATGATGAGAA pLKO_005 235 CDS 100% 4.950 3.465 N Nedd8 n/a
4 TRCN0000012739 GAGATTGAGATAGACATCGAA pLKO.1 128 CDS 100% 3.000 2.100 N Nedd8 n/a
5 TRCN0000273077 GAGATTGAGATAGACATCGAA pLKO_005 128 CDS 100% 3.000 2.100 N Nedd8 n/a
6 TRCN0000012742 CCACAGACAAGGTGGAGCGAA pLKO.1 150 CDS 100% 0.880 0.616 N Nedd8 n/a
7 TRCN0000012740 GCGGCTCATCTACAGTGGCAA pLKO.1 217 CDS 100% 0.880 0.616 N Nedd8 n/a
8 TRCN0000012738 CTGCCAATCATAATGTGGCAT pLKO.1 370 3UTR 100% 0.264 0.185 N Nedd8 n/a
9 TRCN0000284921 CAGCAGCTGATTACAAGATTC pLKO_005 258 CDS 100% 10.800 6.480 N Nedd8 n/a
10 TRCN0000273012 CACCTGGTGTTGGCTCTTAGA pLKO_005 296 CDS 100% 4.950 2.475 Y Nedd8 n/a
11 TRCN0000006500 CAGCAGCTGATTACAAGATTT pLKO.1 258 CDS 100% 13.200 7.920 N NEDD8 n/a
12 TRCN0000342420 CAGCAGCTGATTACAAGATTT pLKO_005 258 CDS 100% 13.200 7.920 N NEDD8 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_008683.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01082 pDONR223 100% 92.6% 98.7% None (many diffs) n/a
2 ccsbBroad304_01082 pLX_304 0% 92.6% 98.7% V5 (many diffs) n/a
3 TRCN0000491483 CATGTCGAAACAGCCTCAAGAGAT pLX_317 100% 92.6% 98.7% V5 (many diffs) n/a
Download CSV