Construct: ORF TRCN0000491498
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016984.2_s317c1
- Derived from:
- ccsbBroadEn_04250
- DNA Barcode:
- CGCCAGCAGACATGCGTCGCACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRMT1L (81627)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491498
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81627 | TRMT1L | tRNA methyltransferase 1 like | NM_030934.5 | 100% | 100% | |
| 2 | human | 81627 | TRMT1L | tRNA methyltransferase 1 like | NM_001202423.1 | 78.7% | 78.7% | 0_1ins468 |
| 3 | human | 81627 | TRMT1L | tRNA methyltransferase 1 like | XM_017002419.2 | 72.8% | 69% | (many diffs) |
| 4 | mouse | 98685 | Trmt1l | tRNA methyltransferase 1 like | NM_026876.3 | 85.8% | 87.7% | (many diffs) |
| 5 | mouse | 98685 | Trmt1l | tRNA methyltransferase 1 like | XM_006529976.2 | 83.4% | 85.4% | (many diffs) |
| 6 | mouse | 98685 | Trmt1l | tRNA methyltransferase 1 like | XM_017313056.1 | 52.4% | 55.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2265
- ORF length:
- 2199
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaatatggcg gaggaggagc tgctgcccct ggagaaggag gaggtggagg 121 tggcccaggt ccaggtcccg accccggccc gggactcggc tggggtccca gctccggccc 181 cggattcggc tctggactcg gctccgactc cggcctcggc tccagcccca gcccctgccc 241 tggcccaggc tccggccctg tccccgtccc tagcctctgc ccctgaggag gctaaaagca 301 agagacacat ctcaattcaa aggcagcttg ctgatctaga gaatttagct tttgtaactg 361 atggaaattt tgactctgcc agctcattga actcagataa tcttgatgca ggcaacagac 421 aggcttgtcc attgtgccct aaggaaaaat tcagagcttg taatagccat aagcttcgtc 481 gtcacctcca gaatttacac tggaaagtct cagttgaatt tgaaggttac aggatgtgca 541 tctgtcactt accttgtcga ccagtgaaac caaacattat tggagaacag ataaccagta 601 aaatgggagc ccattatcat tgtatcattt gttcagcaac aatcaccaga agaactgata 661 tgctaggaca tgttaggcgc cacatgaata aaggagagac taaatctagt tatattgcag 721 cttccactgc taaaccacct aaggaaattt tgaaagaggc agacacggat gtacaagttt 781 gtcccaacta ttctatacct cagaaaacag attcctattt taaccccaaa atgaaactaa 841 atcggcagct aatattctgt acattggctg ctttggctga ggaacgaaaa cctttggaat 901 gtctagatgc ttttggagcc actgggataa tgggattaca gtgggcaaaa catcttggaa 961 atgcagtcaa agttacaatc aatgacttga atgaaaattc tgtgacactg attcaggaaa 1021 actgccattt aaacaaattg aaagtggtgg tggacagtaa ggaaaaggaa aagagtgatg 1081 atattcttga agaaggagag aaaaatcttg gtaatattaa ggtgaccaaa atggatgcca 1141 atgtactgat gcatttgaga tcttttgatt tcatacatct agaccctttt ggaacatcag 1201 tgaattatct agattctgca ttcagaaata taagaaacct tggcatagtg tcagtgactt 1261 ctacagatat cagttcttta tatgccaagg cacagcatgt tgcccggcgt cactacggat 1321 gtaacattgt ccgaactgaa tattacaagg aactagcagc cagaattgtt gtagctgcag 1381 tggcaagagc tgcagcccga tgcaacaaag gcatagaagt actgtttgca gtggctctgg 1441 aacattttgt gttggtagtt gtgagagttt tgaggggacc tacttcagca gatgaaacag 1501 ccaagaagat tcaatacctg atccattgtc agtggtgtga agagagaatt tttcagaagg 1561 atggtaatat ggtagaagaa aacccatata gacagctgcc ttgtaactgt catggaagca 1621 tgcctggaaa gacagcaata gaacttggac ctctgtggtc aagttccctt ttcaatactg 1681 gattcctcaa aagaatgcta tttgaatctc ttcaccatgg tttggatgac attcagaccc 1741 taataaagac attaatcttt gaatcagagt gtacgcctca aagtcagttt tcaattcatg 1801 catcttcaaa tgtcaacaag caagaagaaa atggtgtatt tattaaaact acagatgaca 1861 ccacaacaga taattacatt gcacaaggaa agagaaaaag taatgaaatg atcacaaatt 1921 taggcaagaa gcaaaagact gatgtcagta ctgaacaTCC TCCCTTTTAT TACAACATTC 1981 ACAGACACAG CATTAAAGGA ATGAATATGC CAAAGTTAAA AAAGTTTTTG TGCTATTTAT 2041 CTCAAGCAGG CTTTCGAGTA AGCCGAACTC ATTTTGACCC AATGGGTGTA CGCACAGATG 2101 CACCTCTGAT GCAGTTTAAA TCTATCCTTT TAAAGTACAG CACCCCCACC TACACTGGAG 2161 GACAGTCAGA AAGCCATGTC CAGTCAGCAT CTGAAGATAC AGTAACTGAA AGAGTTGAAA 2221 TGTCAGTGAA TGACAAAGCA GAAGCAAGTG GCTGCAGAAG ATGGTATCCA ACTTTCTTGT 2281 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2341 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2401 GAAAGGACGA CGCCAGCAGA CATGCGTCGC ACATACGCGT TAAGTCgaca atcaacctct 2461 ggattacaaa atttgtgaaa gatt