Construct: ORF TRCN0000491526
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009460.1_s317c1
- Derived from:
- ccsbBroadEn_04890
- DNA Barcode:
- GTTCTTTAGACCGAAATTCTCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRPEL2 (134266)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491526
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 134266 | GRPEL2 | GrpE like 2, mitochondrial | NM_152407.4 | 100% | 100% | |
| 2 | mouse | 17714 | Grpel2 | GrpE-like 2, mitochondrial | NM_021296.2 | 85% | 84.8% | (many diffs) |
| 3 | mouse | 17714 | Grpel2 | GrpE-like 2, mitochondrial | XR_385980.3 | 19% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 741
- ORF length:
- 675
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgtacggtcg ctgtgggcgg gccggctgcg ggtgcagcgc ctactggcct 121 ggagtgccgc gtgggagagc aagggatggc cgcttccatt cagcactgcc acccagagaa 181 ctgctggtga ggactgccgt tctgaggacc ctcctgatga gcttgggccc cctcttgctg 241 aacgagcctt aagggtaaaa gctgttaaac tggagaaaga agtccaagat ttaacagtga 301 gataccagag agctatagct gattgtgaaa acataaggag gcgaacccag agatgtgtgg 361 aagacgccaa gatatttgga atccagagtt tctgtaagga cttggtggag gtggctgaca 421 ttttggagaa gactacagag tgcatttcTG AAGAATCGGA GCCTGAGGAC CAAAAGCTCA 481 CTCTGGAGAA GGTCTTCCGA GGGTTGTTGC TTTTAGAAGC AAAGCTGAAA AGTGTGTTTG 541 CCAAGCATGG CCTGGAGAAA CTGACACCCA TTGGTGACAA ATATGACCCC CATGAGCATG 601 AACTCATCTG TCATGTGCCA GCTGGTGTTG GGGTGCAGCC TGGCACCGTG GCATTAGTAA 661 GACAAGATGG CTACAAACTT CATGGCCGCA CCATTAGGCT TGCCCGAGTG GAAGTGGCAG 721 TGGAGTCTCA GAGAAGACTG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 781 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 841 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTTC TTTAGACCGA 901 AATTCTCTTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt