Construct: ORF TRCN0000491529
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017870.1_s317c1
- Derived from:
- ccsbBroadEn_12800
- DNA Barcode:
- ACTGGCAGGTAGTGCTAAAGGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLDIP3 (84271)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491529
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84271 | POLDIP3 | DNA polymerase delta intera... | XM_017029011.2 | 72.7% | 71.8% | (many diffs) |
| 2 | human | 84271 | POLDIP3 | DNA polymerase delta intera... | XM_017029010.2 | 72.1% | 72.6% | (many diffs) |
| 3 | human | 84271 | POLDIP3 | DNA polymerase delta intera... | NM_001363052.2 | 66% | 66.4% | (many diffs) |
| 4 | human | 84271 | POLDIP3 | DNA polymerase delta intera... | NM_178136.3 | 57.6% | 58% | (many diffs) |
| 5 | human | 84271 | POLDIP3 | DNA polymerase delta intera... | NM_032311.5 | 53.6% | 54% | (many diffs) |
| 6 | human | 84271 | POLDIP3 | DNA polymerase delta intera... | NM_001278657.1 | 51.5% | 51.9% | (many diffs) |
| 7 | human | 84271 | POLDIP3 | DNA polymerase delta intera... | NR_103820.2 | 20% | (many diffs) | |
| 8 | mouse | 73826 | Poldip3 | polymerase (DNA-directed), ... | NM_001347082.1 | 50.3% | 51.2% | (many diffs) |
| 9 | mouse | 73826 | Poldip3 | polymerase (DNA-directed), ... | NM_178627.3 | 46.9% | 47.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 753
- ORF length:
- 687
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggacatctcc ctggacgaac tcatcaggaa gcgcggggcg gcggcgaaag 121 gacggcttaa tgccagaccg ggagttggag gtgtccgatc tcgagttggg atccagcaag 181 gccttctcag ccagtcaaca cgcacagcca ccttccagca gagatttgat gcccggcaga 241 agattggcct ctcagatgcc cggctcaaac tgggagtcaa ggatgcccgg gagaagcttt 301 tgcagaaaga tgcccgattt cGAATCAAAG GGAAAGTGCA GGATGCCAGA GAGATGTTGA 361 ACTCTCGCAA GCAGCAGACC ACGGTGCCCC AGAAGCCCCG CCAGGTTGCT GATGCCCGGG 421 AGAAGATCAG CTTGAAGAGG AGTTCCCCTG CTGCCTTCAT AAACCCACCC ATTGGGACAG 481 TGACCCCTGC TCTGAAGCTC ACCAAAACCA TCCAGAATTT ATATGACCTG GATGAAGATG 541 ATGATGGTAT AGCTTCCGTT CCTACTAAAC AGATGAAGTT TGCAGCCTCA GGCGGCTTTC 601 TCCACCACAT GGCTGGGCTA AGCAGTTCCA AGCTTTCCAT GTCCAAGGCC CTCCCTCTCA 661 CCAAAGTGGT TCAGAATGAT GCATACACAG CTCCTGCTCT CCCTTCCTCT ATTCGAACAA 721 AAGCCCTCCC GGTTCTCACC TCGCCTGGCA CTTACCCAAC TTTCTTGTAC AAAGTGGTTG 781 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 841 GCCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC 901 TGGCAGGTAG TGCTAAAGGA TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 961 ttgtgaaaga tt