Construct: ORF TRCN0000491534
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019691.2_s317c1
- DNA Barcode:
- GGAAATTTTTCTTCTCAGCGAGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NME7 (29922)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491534
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29922 | NME7 | NME/NM23 family member 7 | NM_013330.5 | 99.9% | 99.7% | 1128_1129insG |
| 2 | human | 29922 | NME7 | NME/NM23 family member 7 | NM_197972.2 | 90.3% | 90.1% | 0_1ins108;1020_1021insG |
| 3 | human | 29922 | NME7 | NME/NM23 family member 7 | NR_104229.1 | 65.6% | 1_253del;1244_1306del;1445_1719delinsG | |
| 4 | mouse | 171567 | Nme7 | NME/NM23 family member 7 | NM_001293313.1 | 81.1% | 83.8% | (many diffs) |
| 5 | mouse | 171567 | Nme7 | NME/NM23 family member 7 | NM_138314.4 | 81.1% | 83.8% | (many diffs) |
| 6 | mouse | 171567 | Nme7 | NME/NM23 family member 7 | XM_006496692.2 | 77.2% | 79.5% | (many diffs) |
| 7 | mouse | 171567 | Nme7 | NME/NM23 family member 7 | XM_006496693.3 | 77.2% | 79.5% | (many diffs) |
| 8 | mouse | 171567 | Nme7 | NME/NM23 family member 7 | NM_178071.6 | 56% | 56.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1206
- ORF length:
- 1131
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgaat catagtgaaa gattcgtttt cattgcagag tggtatgatc 121 caaatgcttc acttcttcga cgttatgagc ttttatttta cccaggggat ggatctgttg 181 aaatgcatga tgtaaagaat catcgcacct ttttaaagcg gaccaaatat gataacctgc 241 acttggaaga tttatttata ggcaacaaag tgaatgtctt ttctcgacaa ctggtattaa 301 ttgactatgg ggatcaatat acagctcgcc agctgggcag taggaaagaa aaaacgctag 361 ccctaattaa accagatgca atatcaaagg ctggagaaat aattgaaata ataaacaaag 421 ctggatttac tataaccaaa ctcaaaatga tgatgctttc aaggaaagaa gcattggatt 481 ttcatgtaga tcaccagtca agaccctttt tcaatgagct gatccagttt attacaactg 541 gtcctattat tgccatggag attttaagag atgatgctat atgtgaatgg aaaagactgc 601 tgggacctgc aaactctgga gtggcacgca cagatgcttc tgaaagcatt agagccctct 661 ttggaacaga tggcataaga aatgcagcgc atggccctga ttcttttgct tctgcggcca 721 gagaaatgga gttgtttttt ccttcaagtg gaggttgtgg gccggcaaac actgctaaat 781 ttactaattg tacctgttgc attgttaaac cccatgctgt cagtgaagga ctgttgggaa 841 agatcctgat ggctatccga gatgcaggtt ttgaaatcTC AGCTATGCAG ATGTTCAATA 901 TGGATCGGGT TAATGTTGAG GAATTCTATG AAGTTTATAA AGGAGTAGTG ACCGAATATC 961 ATGACATGGT GACAGAAATG TATTCTGGCC CTTGTGTAGC AATGGAGATT CAACAGAATA 1021 ATGCTACAAA GACATTTCGA GAATTTTGTG GACCTGCTGA TCCTGAAATT GCCCGGCATT 1081 TACGCCCTGG AACTCTCAGA GCAATCTTTG GTAAAACTAA GATCCAGAAT GCTGTTCACT 1141 GTACTGATCT GCCAGAGGAT GGCCTATTAG AGGTTCAATA CTTCTTCAAG ATCTTGGATA 1201 ATGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1261 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1321 GGCTTTATAT ATCTTGTGGA AAGGACGAGG AAATTTTTCT TCTCAGCGAG CAACGCGTTA 1381 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt