Construct: ORF TRCN0000491589
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019819.2_s317c1
- DNA Barcode:
- CTAAACCTTTCCAAATGAGTCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PNCK (139728)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491589
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_024452339.1 | 28.6% | 25.7% | (many diffs) |
| 2 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001366975.1 | 24.8% | 24.7% | 68_69ins87;278_1029del |
| 3 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001366976.1 | 24.8% | 24.7% | 68_69ins87;278_1029del |
| 4 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001366977.1 | 24.8% | 24.7% | 68_69ins87;278_1029del |
| 5 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001366978.1 | 24.8% | 24.7% | 68_69ins87;278_1029del |
| 6 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001366979.1 | 24.8% | 24.7% | 68_69ins87;278_1029del |
| 7 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001366980.1 | 24.8% | 24.7% | 68_69ins87;278_1029del |
| 8 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_006724809.3 | 24.8% | 24.7% | 68_69ins87;278_1029del |
| 9 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_011531107.2 | 24.8% | 24.7% | 68_69ins87;278_1029del |
| 10 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_011531111.2 | 24.8% | 24.7% | 68_69ins87;278_1029del |
| 11 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_011531112.2 | 24.8% | 24.7% | 68_69ins87;278_1029del |
| 12 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_017029279.1 | 24.8% | 24.7% | 68_69ins87;278_1029del |
| 13 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_024452340.1 | 24.8% | 24.7% | 68_69ins87;278_1029del |
| 14 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001135740.1 | 23.7% | 23.6% | 1_51del;119_120ins87;329_1080del |
| 15 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_011531108.2 | 23.6% | 23.5% | 1_54del;122_123ins87;332_1083del |
| 16 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001039582.3 | 20.2% | 20.2% | 1_249del;317_318ins87;527_1278del |
| 17 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | NM_001199351.1 | 21.5% | 23.9% | (many diffs) |
| 18 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | NM_001199352.1 | 21.5% | 23.9% | (many diffs) |
| 19 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | NM_012040.3 | 21.5% | 23.9% | (many diffs) |
| 20 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XM_006528344.4 | 21.5% | 23.9% | (many diffs) |
| 21 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XM_011247692.3 | 21.5% | 23.9% | (many diffs) |
| 22 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XM_030251530.1 | 21.5% | 23.9% | (many diffs) |
| 23 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XM_030251531.1 | 21.5% | 23.9% | (many diffs) |
| 24 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XR_003953034.1 | 16.1% | (many diffs) | |
| 25 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XR_001782761.2 | 15.5% | (many diffs) | |
| 26 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XR_003953033.1 | 14.1% | (many diffs) | |
| 27 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XR_003953032.1 | 14% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 435
- ORF length:
- 366
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gctgctgctg aagaaacaca cggaggacat cagcagcgtc TACGAGATCC 121 GCGAGAGGCT CGGCTCGGGG CCCTCCCCGC TCCACTCTCT CTCCCTCCTG CCTCTCCTCT 181 CTTCCCACTT CCTTCCCACC TCCCACCGCC CCGTCTGCGG CAGGGGTGCC TTCTCCGAGG 241 TGGTGCTGGC CCAGGAGCGG GGCTCCGCAC ACCTCGTGGC CCTCAAGTGC ATCCCCAAGA 301 AGGCCCTCCG GGGCAAGGAG GCCCTGGTGG AGAACGAGAT CGCAGTGCTC CGTAGGATCA 361 GTCACCCCAA CATCGTCGCT CTGGAGGATG TCCACGAGAG CCCTTCCCAC CTCTACCTGG 421 CCATGGAACT GGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 481 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 541 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACTA AACCTTTCCA AATGAGTCAC 601 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t