Construct: ORF TRCN0000491596
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000952.2_s317c1
- Derived from:
- ccsbBroadEn_02684
- DNA Barcode:
- GGATACATCCTATAATGTGCATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IKZF3 (22806)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491596
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22806 | IKZF3 | IKAROS family zinc finger 3 | NM_012481.5 | 100% | 100% | |
| 2 | human | 22806 | IKZF3 | IKAROS family zinc finger 3 | NM_001257408.2 | 93.3% | 93.3% | 59_60ins102 |
| 3 | human | 22806 | IKZF3 | IKAROS family zinc finger 3 | NM_183229.3 | 92.3% | 92.3% | 589_590ins117 |
| 4 | human | 22806 | IKZF3 | IKAROS family zinc finger 3 | NM_183230.3 | 92.3% | 92.3% | 707_708ins117 |
| 5 | human | 22806 | IKZF3 | IKAROS family zinc finger 3 | NM_183228.3 | 88.9% | 88.9% | 421_422ins168 |
| 6 | human | 22806 | IKZF3 | IKAROS family zinc finger 3 | NM_001257409.2 | 85.6% | 85.6% | 59_60ins102;487_488ins117 |
| 7 | human | 22806 | IKZF3 | IKAROS family zinc finger 3 | NM_183232.3 | 84.6% | 84.6% | 590_591ins234 |
| 8 | human | 22806 | IKZF3 | IKAROS family zinc finger 3 | NM_001257410.2 | 82.9% | 82.9% | 162_163ins261 |
| 9 | human | 22806 | IKZF3 | IKAROS family zinc finger 3 | NM_183231.3 | 81.3% | 81.3% | 420_421ins285 |
| 10 | human | 22806 | IKZF3 | IKAROS family zinc finger 3 | NM_001257414.2 | 73.6% | 73.6% | 422_423ins402 |
| 11 | human | 22806 | IKZF3 | IKAROS family zinc finger 3 | NM_001257411.2 | 71.9% | 71.9% | 162_163ins429 |
| 12 | human | 22806 | IKZF3 | IKAROS family zinc finger 3 | NM_001257412.2 | 64.2% | 64.2% | 162_163ins546 |
| 13 | human | 22806 | IKZF3 | IKAROS family zinc finger 3 | NM_001257413.2 | 56.5% | 56.5% | 162_163ins663 |
| 14 | human | 22806 | IKZF3 | IKAROS family zinc finger 3 | NM_001284514.1 | 51.4% | 51.4% | 0_1ins741 |
| 15 | human | 22806 | IKZF3 | IKAROS family zinc finger 3 | NM_001284515.2 | 51.4% | 51.4% | 0_1ins741 |
| 16 | human | 22806 | IKZF3 | IKAROS family zinc finger 3 | NM_001284516.1 | 51.4% | 51.4% | 0_1ins741 |
| 17 | mouse | 22780 | Ikzf3 | IKAROS family zinc finger 3 | NM_011771.1 | 86.1% | 86.6% | (many diffs) |
| 18 | mouse | 22780 | Ikzf3 | IKAROS family zinc finger 3 | XM_006533204.4 | 83.3% | 84% | (many diffs) |
| 19 | mouse | 22780 | Ikzf3 | IKAROS family zinc finger 3 | XM_006533206.4 | 49.8% | 48.6% | (many diffs) |
| 20 | mouse | 22780 | Ikzf3 | IKAROS family zinc finger 3 | XM_006533205.4 | 49.3% | 48.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1593
- ORF length:
- 1527
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agatatacaa acaaatgcgg aactgaaaag cactcaggag cagtctgtgc 121 ccgcagaaag tgcagcggtt ttgaatgact acagtttaac caaatctcat gaaatggaaa 181 atgtggacag tggagaaggc ccagccaatg aagatgaaga cataggagat gattcaatga 241 aagtgaaaga tgaatacagt gaaagagatg agaatgtttt aaagtcagaa cccatgggaa 301 atgcagaaga gcctgaaatc ccttacagct attcaagaga atataatgaa tatgaaaaca 361 ttaagttgga gagacatgtt gtctcattcg atagtagcag gccaaccagt ggaaagatga 421 actgcgatgt gtgtggatta tcctgcatca gcttcaatgt cttaatggtt cataagcgaa 481 gccatactgg tgaacgccca ttccagtgta atcagtgtgg ggcatctttt actcagaaag 541 gtaacctcct ccgccacatt aaactgcaca caggggaaaa accttttaag tgtcacctct 601 gcaactatgc atgccaaaga agagatgcgc tcacggggca tcttaggaca cattctgtgg 661 agaaacccta caaatgtgag ttttgtggaa ggagttacaa gcagagaagt tcccttgagg 721 agcacaagga gcgctgccgt acatttcttc agagcactga cccaggggac actgcaagtg 781 cggaggcaag acacatcaaa gcagagatgg gaagtgaaag agctctcgta ctggacagat 841 tagcaagcaa tgtggcaaaa cgaaaaagct caatgcctca gaaattcatt ggtgagaagc 901 gccactgctt tgatgtcaac tataattcaa gttacatgta tgagaaagag agtgagctca 961 tacagacccg catgatggac caagccatca ataacgccat cagctatctt ggcgccgaag 1021 ccctgcgccc cttggtccag acaccgcctg ctcccacctc ggagatggtt ccagttatca 1081 gcagcatgta tcccatagcc ctcacccggg ctgagatgtc aaacggtgcc cctcaagagc 1141 tggaaaagaa aagcatccac cttccagaga agagcgtgcc ttcTGAGAGA GGCCTCTCTC 1201 CCAACAATAG TGGCCACGAC TCCACGGACA CTGACAGCAA CCATGAAGAA CGCCAGAATC 1261 ACATCTATCA GCAAAATCAC ATGGTCCTGT CTCGGGCCCG CAATGGGATG CCACTTCTGA 1321 AGGAGGTTCC CCGCTCTTAC GAACTCCTCA AGCCCCCGCC CATCTGCCCA AGAGACTCCG 1381 TCAAAGTGAT CAACAAGGAA GGGGAGGTGA TGGATGTGTA TCGGTGTGAC CACTGCCGCG 1441 TCCTCTTCCT GGACTATGTG ATGTTCACGA TTCACATGGG CTGCCACGGC TTCCGTGACC 1501 CTTTCGAGTG TAACATGTGT GGATATCGAA GCCATGATCG GTATGAGTTC TCGTCTCACA 1561 TAGCCAGAGG AGAACACAGA GCCCTGCTGA AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1621 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1681 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGG 1741 ATACATCCTA TAATGTGCAT TAACGCGTTA AGTCgacaat caacctctgg attacaaaat 1801 ttgtgaaaga tt