Construct: ORF TRCN0000491599
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020271.2_s317c1
- DNA Barcode:
- GGACTGGACCCGACACTCACGCAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NME6 (10201)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491599
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | NM_005793.4 | 100% | 100% | |
| 2 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_017005513.2 | 97.9% | 96.9% | 4_5insCCCAGAATCTGG |
| 3 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | NM_001308426.1 | 95.8% | 95.8% | 0_1ins24 |
| 4 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | NM_001308427.1 | 95.8% | 95.8% | 0_1ins24 |
| 5 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | NM_001308428.1 | 95.8% | 95.8% | 0_1ins24 |
| 6 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_017005514.2 | 95.8% | 95.8% | 0_1ins24 |
| 7 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | NM_001308430.1 | 88.1% | 41.2% | 215_216ins40;513_514ins29 |
| 8 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | NM_001308431.1 | 84% | 37.7% | 0_1ins24;191_192ins40;489_490ins29 |
| 9 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | NM_001308433.1 | 84% | 37.7% | 0_1ins24;191_192ins40;489_490ins29 |
| 10 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_017005515.1 | 84% | 37.7% | 0_1ins24;191_192ins40;489_490ins29 |
| 11 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | NM_001308434.1 | 72.6% | 72.6% | 0_1ins159 |
| 12 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_017005516.2 | 66.1% | 39.2% | (many diffs) |
| 13 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_017005511.2 | 62% | 35.4% | (many diffs) |
| 14 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_024453300.1 | 61.9% | 50.5% | (many diffs) |
| 15 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | NM_001308435.1 | 61.3% | 61.3% | 0_1ins24;192_193ins201 |
| 16 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_024453301.1 | 60.5% | 48.4% | (many diffs) |
| 17 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_024453297.1 | 59.1% | 47.7% | (many diffs) |
| 18 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_024453302.1 | 59.1% | 47.7% | (many diffs) |
| 19 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_024453303.1 | 59.1% | 47.7% | (many diffs) |
| 20 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_024453304.1 | 59.1% | 47.7% | (many diffs) |
| 21 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_024453305.1 | 59.1% | 47.7% | (many diffs) |
| 22 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_024453306.1 | 59.1% | 47.7% | (many diffs) |
| 23 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_017005517.1 | 57.3% | 56.7% | (many diffs) |
| 24 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_017005512.2 | 57.2% | 42.6% | (many diffs) |
| 25 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_017005518.2 | 57.2% | 57.2% | 0_1ins249 |
| 26 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_024453299.1 | 54.8% | 45% | (many diffs) |
| 27 | mouse | 54369 | Nme6 | NME/NM23 nucleoside diphosp... | NM_018757.1 | 82.6% | 84.2% | (many diffs) |
| 28 | mouse | 54369 | Nme6 | NME/NM23 nucleoside diphosp... | XM_006512197.1 | 82.6% | 84.2% | (many diffs) |
| 29 | mouse | 54369 | Nme6 | NME/NM23 nucleoside diphosp... | XM_006512198.3 | 82.6% | 84.2% | (many diffs) |
| 30 | mouse | 54369 | Nme6 | NME/NM23 nucleoside diphosp... | XM_006512199.3 | 82.6% | 84.2% | (many diffs) |
| 31 | mouse | 54369 | Nme6 | NME/NM23 nucleoside diphosp... | XM_006512200.2 | 52.7% | 52.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 654
- ORF length:
- 582
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgacccag aatctgggga gtgagatggc ctcaatcttg cgaagccctc 121 aggctctcca gctcactcta gccctgatca agcctgacgc agtcgcccat ccactgattc 181 tggaggctgt tcatcagcag attctaagca acaagttcct gattgtacga atgagagaac 241 tactgtggag aaaggaagat tgccagaggt tttaccgaga gcatgaaggg cgttttttct 301 atcagaggct ggtggagttc atggccagcg ggccaatccg agcctacatc cttgcccaca 361 aggatgccat ccagctctgg aggacgcTCA TGGGACCCAC CAGAGTGTTC CGAGCACGCC 421 ATGTGGCCCC AGATTCTATC CGTGGGAGTT TCGGCCTCAC TGACACCCGC AACACCACCC 481 ATGGTTCGGA CTCTGTGGTT TCAGCCAGCA GAGAGATTGC AGCCTTCTTC CCTGACTTCA 541 GTGAACAGCG CTGGTATGAG GAGGAAGAGC CCCAGTTGCG CTGTGGCCCT GTGTGCTATA 601 GCCCAGAGGG AGGTGTCCAC TATGTAGCTG GAACAGGAGG CCTAGGACCA GCCTGAGACC 661 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 721 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 781 ATATATCTTG TGGAAAGGAC GAGGACTGGA CCCGACACTC ACGCAGACGC GTTAAGTCga 841 caatcaacct ctggattaca aaatttgtga aagatt