Construct: ORF TRCN0000491604
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007221.1_s317c1
- Derived from:
- ccsbBroadEn_13261
- DNA Barcode:
- GCGTCTCGTGCTCTTGGCTTGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZDHHC8P1 (150244)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491604
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29801 | ZDHHC8 | zinc finger DHHC-type conta... | NM_013373.4 | 13.2% | 12.4% | (many diffs) |
| 2 | human | 29801 | ZDHHC8 | zinc finger DHHC-type conta... | NM_001185024.2 | 13% | 12.2% | (many diffs) |
| 3 | human | 29801 | ZDHHC8 | zinc finger DHHC-type conta... | XM_006724239.2 | 13% | 12.2% | (many diffs) |
| 4 | human | 150244 | ZDHHC8P1 | zinc finger DHHC-type conta... | NR_003950.1 | 12.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 402
- ORF length:
- 333
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCACCAT GGCATCCATC CAGGAGCGCA AGGACAGGGA GGAGCGTGAG TGCCTGCTGC 121 GCTCCCAGGC CGACTCACTC TTCGGCGACT CAGGCGTCTA TGATGCTCCC AGCTCCTACA 181 GCCTGCAGCA GGCCAGTGTG CTGTCTGAGG GCTTCCGAGG TCCTACGCTG TGCTACAGCT 241 CTACAGATGA CCTTGTGGCC AGGCCCGGCT TCGGCGGCGC CTGCAACCCT GTCCTGCAGA 301 CATCATTGTC CTCGCTTTCC AGCTCCGTGA GCCGTGCACT GCGGACGTCG TCCTCCTCCC 361 TGCAGGCTGA TCAGGTGGGG AAGCTGAGGC AGGAAGCCCT TTTGCCAACT TTCTTGTACA 421 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 481 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 541 AGGACGAGCG TCTCGTGCTC TTGGCTTGCG GACGCGTTAA GTCgacaatc aacctctgga 601 ttacaaaatt tgtgaaagat t