Construct: ORF TRCN0000491617
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020227.2_s317c1
- DNA Barcode:
- TGTCAAAACTACAACGGAACACGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PIK3CB (5291)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491617
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | NM_006219.3 | 100% | 100% | |
| 2 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_006713659.3 | 100% | 100% | |
| 3 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_011512895.2 | 100% | 100% | |
| 4 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_017006619.1 | 100% | 100% | |
| 5 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_024453596.1 | 93% | 93% | 0_1ins171;1359_1360ins51 |
| 6 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XR_002959542.1 | 64.4% | 0_1ins155;1879_1880ins100;2956_4331del | |
| 7 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_024453597.1 | 62.9% | 62.9% | 0_1ins1188 |
| 8 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_017006621.2 | 61.4% | 61.4% | 0_1ins1188;342_343ins51 |
| 9 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | NM_001256045.1 | 54.3% | 54.3% | 0_1ins1413;117_118ins51 |
| 10 | mouse | 74769 | Pik3cb | phosphatidylinositol-4,5-bi... | NM_029094.3 | 87.9% | 95.3% | (many diffs) |
| 11 | mouse | 74769 | Pik3cb | phosphatidylinositol-4,5-bi... | XM_011242829.2 | 87.9% | 95.3% | (many diffs) |
| 12 | mouse | 74769 | Pik3cb | phosphatidylinositol-4,5-bi... | XM_011242830.2 | 87.9% | 95.3% | (many diffs) |
| 13 | mouse | 74769 | Pik3cb | phosphatidylinositol-4,5-bi... | XM_006511520.2 | 75.6% | 82.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3282
- ORF length:
- 3210
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtgcttc agtttcataa tgcctcctgc tatggcagac atccttgaca 121 tctgggcggt ggattcacag atagcatctg atggctccat acctgtggat ttccttttgc 181 ccactgggat ttatatccag ttggaggtac ctcgggaagc taccatttct tatattaagc 241 agatgttatg gaagcaagtt cacaattacc caatgttcaa cctccttatg gatattgact 301 cctatatgtt tgcatgtgtg aatcagactg ctgtatatga ggagcttgaa gatgaaacac 361 gaagactctg tgatgtcaga ccttttcttc cagttctcaa attagtgaca agaagttgtg 421 acccagggga aaaattagac tcaaaaattg gagtccttat aggaaaaggt ctgcatgaat 481 ttgattcctt gaaggatcct gaagtaaatg aatttcgaag aaaaatgcgc aaattcagcg 541 aggaaaaaat cctgtcactt gtgggattgt cttggatgga ctggctaaaa caaacatatc 601 caccagagca tgaaccatcc atccctgaaa acttagaaga taaactttat gggggaaagc 661 tcatcgtagc tgttcatttt gaaaactgcc aggacgtgtt tagctttcaa gtgtctccta 721 atatgaatcc tatcaaagta aatgaattgg caatccaaaa acgtttgact attcatggga 781 aggaagatga agttagcccc tatgattatg tgttgcaagt cagcgggaga gtagaatatg 841 tttttggtga tcatccacta attcagttcc agtatatccg gaactgtgtg atgaacagag 901 ccctgcccca ttttatactt gtggaatgct gcaagatcaa gaaaatgtat gaacaagaaa 961 tgattgccat agaggctgcc ataaatcgaa attcatctaa tcttcctctt ccattaccac 1021 caaagaaaac acgaattatt tctcatgttt gggaaaataa caaccctttc caaattgtct 1081 tggttaaggg aaataaactt aacacagagg aaactgtaaa agttcatgtc agggctggtc 1141 tttttcatgg tactgagctc ctgtgtaaaa ccatcgtaag ctcagaggta tcagggaaaa 1201 atgatcatat ttggaatgaa ccactggaat ttgatattaa tatttgtgac ttaccaagaa 1261 tggctcgatt atgttttgct gtttatgcag ttttggataa agtaaaaacg aagaaatcaa 1321 cgaaaactat taatccctct aaatatcaga ccatcaggaa agctggaaaa gtgcattatc 1381 ctgtagcgtg ggtaaatacg atggtttttg actttaaagg acaattgaga actggagaca 1441 taatattaca cagctggtct tcatttcctg atgaactcga agaaatgttg aatccaatgg 1501 gaactgttca aacaaatcca tatactgaaa atgcaacagc tttgcatgtt aaatttccag 1561 agaataaaaa acaaccttat tattaccctc ccttcgataa gattattgaa aaggcagctg 1621 agattgcaag cagtgatagt gctaatgtgt caagtcgagg tggaaaaaag tttcttcctg 1681 tattgaaaga aatcttggac agggatccct tgtctcaact gtgtgaaaat gaaatggatc 1741 ttatttggac tttgcgacaa gactgccgag agattttccc acaatcactg ccaaaattac 1801 tgctgtcaat caagtggaat aaacttgagg atgttgctca gcttcaggcg ctgcttcaga 1861 tttggcctaa actgcccccc cgggaggccc tagagcttct ggatttcaac tatccagacc 1921 agtacgttcg agaatatgct gtaggctgcc tgcgacagat gagtgatgaa gaactttctc 1981 aatatctttt acaactggtg caagtgttaa aatatgagcc ttttcttgat tgtgccctct 2041 ctagattcct attagaaaga gcacttggta atcggaggat agggcagttt ctattttggc 2101 atcttaggtc agaagtgcac attcctgctg tctcagtaca atttggtgtc atccttgaag 2161 catactgccg gggaagtgtg gggcacatga aagtgctttc taagcaggtt gaagcactca 2221 ataagttaaa aactttaaat agtttaatca aactgaatgc cgtgaagtta aacagagcca 2281 aagggaagga ggccatgcat acctgtttaa aacagagtgc ttaccgggaa gccctctctg 2341 acctgcagtc acccctgaac ccatgtgtta tcctctcaga actctatgtt gaaaagtgca 2401 aatacatgga ttccaaaatg aagcctttgt ggctggtata caataacaag gtatttggtg 2461 aggattcagt tggagtgatt tttaaaaatg gtgatgattt acgacaggat atgttgacac 2521 tccaaatgtt gcgcttgatg gatttactct ggaaagaagc tggtttggat cttcggatgt 2581 tgccttatgg ctgtttagca acaggagatc gctctggcct cattgaagtt gtgagcacct 2641 ctgaaacaat tgctgacatt cagctgaaca gtagcaatgt ggctgctgca gcagccttca 2701 acaaagatgc ccttctgaac tggcttaaag aatacaactc tggggatgac ctggaccgag 2761 ccattgagga atttacactg tcctgtgctg gctactgtgt agcttcttat gtccttggga 2821 ttggtgacag acatagtgac aacatcatgg tcaaaaaaac tggccagctc ttccacattg 2881 actttggaca tattcttGGA AATTTCAAAT CTAAGTTTGG CATTAAAAGG GAGCGAGTGC 2941 CTTTTATTCT TACCTATGAT TTCATCCATG TCATTCAACA AGGAAAAACA GGAAATACAG 3001 AAAAGTTTGG CCGGTTCCGC CAGTGTTGTG AGGATGCATA TCTGATTTTA CGACGGCATG 3061 GGAATCTCTT CATCACTCTC TTTGCGCTGA TGTTGACTGC AGGGCTTCCT GAACTCACAT 3121 CAGTCAAAGA TATACAGTAT CTTAAGGACT CTCTTGCATT AGGGAAGAGT GAAGAAGAAG 3181 CACTCAAACA GTTTAAGCAA AAATTTGATG AGGCGCTCAG GGAAAGCTGG ACTACTAAAG 3241 TGAACTGGAT GGCCCACACA GTTCGGAAAG ACTACAGATC TTGAGACCCA GCTTTCTTGT 3301 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 3361 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 3421 GAAAGGACGA TGTCAAAACT ACAACGGAAC ACGCACGCGT TAAGTCgaca atcaacctct 3481 ggattacaaa atttgtgaaa gatt